A5079878245- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Agricultural pest management studies
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Insect Pest Control Strategies
- Entomopathogenic Microorganisms in Pest Control
- Insect Utilization and Effects
- Biochemical and Structural Characterization
- Legume Nitrogen Fixing Symbiosis
- Insect behavior and control techniques
- Plant Pathogenic Bacteria Studies
- Health, Medicine and Society
- Historical and Scientific Studies
- Phytoplasmas and Hemiptera pathogens
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Microbial Metabolic Engineering and Bioproduction
- Nematode management and characterization studies
- Forest Insect Ecology and Management
- Banana Cultivation and Research
- Animal Genetics and Reproduction
- Transgenic Plants and Applications
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2017-2025
Laboratoire de Microbiologie, Adaptation et Pathogénie
1988-2025
Plant Health Institute de Montpellier
2021-2024
Centre Occitanie-Montpellier
2021-2024
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2018-2024
Département Santé des Plantes et Environnement
2017-2023
Biologie et Génétique des Interactions Plante-Parasite
2018-2021
AgroParisTech
2010-2021
Institut National des Sciences Appliquées de Lyon
2004-2020
Université Claude Bernard Lyon 1
2009-2020
Aphids are important agricultural pests and also biological models for studies of insect-plant interactions, symbiosis, virus vectoring, the developmental causes extreme phenotypic plasticity. Here we present 464 Mb draft genome assembly pea aphid Acyrthosiphon pisum. This first published whole sequence a basal hemimetabolous insect provides an outgroup to multiple genomes holometabolous insects. Pea aphids host-plant specialists, they can reproduce both sexually asexually, have coevolved...
Abstract Background Recent genomic analyses of arthropod defense mechanisms suggest conservation key elements underlying responses to pathogens, parasites and stresses. At the center pathogen-induced immune are signaling pathways triggered by recognition fungal, bacterial viral signatures. These result in production response molecules, such as antimicrobial peptides lysozymes, which degrade or destroy invaders. Using recently sequenced genome pea aphid ( Acyrthosiphon pisum ), we conducted...
Abstract Potato plants ( Solanum tuberosum ) cv. Desireé were transformed with the genes encoding proteins bean chitinase (BCH), snowdrop lectin (GNA) and wheat α‐amylase inhibitor (WAI) under control of constitutive CaMV 35S promoter. Transgenic detectable levels foreign RNA then selected for further characterisation respect to protein expression by immunodot blot analysis using polyclonal antibodies raised against respective protein. With exception WAI, expressing high RNA, expressed...
Abstract Acute toxicity of thirty lectins was tested against the pea aphid Acyrthosiphon pisum (Harris) (Homoptera, Aphididae: Macrosiphini). Activity measured on artificial diets containing moderate concentrations (10–250 μg/ml) by scoring mortality and growth inhibition over whole nymphal period (7 days at 20°C). Most proteins exhibited low toxicity, but some induced significant mortality; these included from jackbean (Concanavalin A), amaranth, lentil snowdrop. There no direct correlation...
A green biotype of the pea aphid, A. pisum, from Lusignan (France), showed very poor performance on standard Akey and Beck diet. Significant improvement occurred after reduction osmotic pressure diet modification amino acid component, according to results carcass analysis. further was obtained through an optimalization aromatic level: tyrosine deficiency induced by its solubility overcome excess phenylalanine inclusion a soluble compound, β-alanyltyrosine. new therefore formulated for this...
Abstract Three mannose‐binding lectins were assayed in artificial diets for their toxic and growth‐inhibitory effects on nymphal development of the peach‐potato aphid Myzus persicae. The snowdrop ( Galanthus nivalis ) lectin GNA was most toxic, with an induced mortality 42% at 1500 μg ml −1 (30 μM) IC 50 (50% growth inhibition) 630 (13 μM). daffodil Narcissus pseudonarcissus NPA a garlic Allium sativum ASA no significant range 10–1500 , but did result inhibition 59% (NPA) 26% (ASA) (40 μM...
Abstract Recent progress in plant transformation for insect resistance has increased the interest potential toxicity of proteins towards pests. While studies have been targeted to a large array species, phloem‐feeding Homoptera not investigated yet. The paper describes routine test screening and growth inhibition purified artificial diets on Acyrthosiphon pisum (Harris). Twenty‐five commercially available different classes were tested compared some non‐protein chemicals (an insecticide, an...
Abstract Aphids are the leading pests in agricultural crops. A large-scale sequencing of 40,904 ESTs from pea aphid Acyrthosiphon pisum was carried out to define a catalog 12,082 unique transcripts. strong AT bias found, indicating compositional shift between Drosophila melanogaster and A. . An silico profiling analysis characterized 135 transcripts specific pea-aphid tissues (relating bacteriocytes parthenogenetic embryos). This project is first address genetics Hemiptera hemimetabolous insect.
ABSTRACT Dickeya dadantii ( Erwinia chrysanthemi ) is a phytopathogenic bacterium causing soft rot diseases on many crops. The sequencing of its genome identified four genes encoding homologues the Cyt family insecticidal toxins from Bacillus thuringiensis , which are not present in close relative Pectobacterium carotovorum subsp. atrosepticum . pathogenicity D. was tested pea aphid Acyrthosiphon pisum and shown to be highly virulent for this insect, either by septic injury or oral...
Abstract Phloem Protein2 (PP2) is a component of the phloem protein bodies found in sieve elements. We describe here lectin properties Arabidopsis (Arabidopsis thaliana) PP2-A1. Using recombinant produced Escherichia coli, we demonstrated binding to N-acetylglucosamine oligomers. Glycan array screening showed that PP2-A1 also bound high-mannose N-glycans and 9-acyl-N-acetylneuraminic sialic acid. Fluorescence spectroscopy-based titration experiments revealed had two classes site for...
ABSTRACT Four Bacillus thuringiensis δ-endotoxins, Cry3A, Cry4Aa, Cry11Aa, and Cyt1Aa, were found to exhibit low moderate toxicity on the pea aphid, Acyrthosiphon pisum , in terms both of mortality growth rate. Cry1Ab was essentially nontoxic except at high rates. To demonstrate these effects, we had use exhaustive buffer-based controls.
RNA interference (RNAi) has been widely and successfully used for gene inactivation in insects, including aphids, where dsRNA administration can be performed either by feeding or microinjection. However, several aspects related to the aphid response RNAi, as well influence of method on tissue response, mixed success observe phenotypes specific targeted, are still unclear this insect group. In present study, we made first direct comparison two methods (injection feeding) delivery targeting...
Abstract Background Although native to North America, the invasion of aphid-like grape phylloxera Daktulosphaira vitifoliae across globe altered course cultivation. For past 150 years, viticulture relied on grafting-resistant American Vitis species as rootstocks, thereby limiting genetic stocks tolerant other stressors such pathogens and climate change. Limited understanding insect genetics resulted in successive outbreaks when rootstocks failed. Here we report 294-Mb genome D. a basic tool...
The fate of sucrose, the major nutrient an aphid's natural food, was explored by radiolabeling in pea aphid Acyrthosiphon pisum. To investigate influence nitrogen quality food on amino acid neosynthesis, aphids were reared two artificial diets differing their composition. first (diet A) had equilibrated balance, similar to that derived from analysis carcass, and other B) unbalanced composition legume phloem sap. Aphids grown either diet expired same quantity sucrose carbon as CO(2),...
Abstract BACKGROUND: Saponins are a class of secondary plant metabolites consisting sugar moiety glycosidically linked to hydrophobic aglycone (sapogenin) that often possess insecticidal activities. Four saponins were selected: two triterpene saponins, Q. saponaria and aescin, steroidal digitonin diosgenin. Their effects investigated on an important pest species model piercing‐sucking insect, the pea aphid Acyrthosiphon pisum. The bark saponin received special attention because its high...
A single membrane‐bound aminopeptidase N (APN) occurs in the pea aphid ( Acyrthosiphon pisum Harris) midgut, with a pH optimum of 7.0, pI 8.1 and molecular mass 130 kDa. This enzyme accounts for more than 15.6% total gut proteins. After being solubilized detergent, APN was purified to homogeneity. The is glycoprotein rich mannose residues, which binds entomotoxic lectins concanavalin family. internal sequence homologous conservative domain APNs, degenerated primers highly conserved motifs...
Abstract We have characterized proteinase activities in gut extracts from the cotton‐melon aphid ( Aphis gossypii Glover), an insect feeding strictly on protein‐poor phloem. The major, if not exclusive, intestinal proteinases of this are cysteine type. A cDNA has been cloned a library and codes for AgCatL, cathepsin L‐like proteinase. AgCatL protein shows high sequence similarity with mammalian some arthropod proteinases, but can be reliably distinguished secreted (digestive) identified...