A5029794699- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Insect Pheromone Research and Control
- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Plant and animal studies
- Insect Utilization and Effects
- Insect and Pesticide Research
- Olfactory and Sensory Function Studies
- Lepidoptera: Biology and Taxonomy
- Entomopathogenic Microorganisms in Pest Control
- Date Palm Research Studies
- Plant Virus Research Studies
- Insect Pest Control Strategies
- Invertebrate Immune Response Mechanisms
- Forest Insect Ecology and Management
- Insect behavior and control techniques
- Antimicrobial Peptides and Activities
- Plant biochemistry and biosynthesis
- Entomological Studies and Ecology
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Silk-based biomaterials and applications
- Animal Behavior and Reproduction
Institut d'écologie et des sciences de l'environnement de Paris
2016-2025
Institut de Recherche pour le Développement
2016-2025
Université Paris Cité
2009-2025
Centre National de la Recherche Scientifique
2018-2025
Sorbonne Université
2016-2025
Université Paris-Est Créteil
2015-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2017-2025
AgroParisTech
2007-2019
Biocon (Switzerland)
2019
Institut National de la Recherche Agronomique
1999-2015
The evolutionary importance of hybridization and introgression has long been debated. Hybrids are usually rare unfit, but even infrequent can aid adaptation by transferring beneficial traits between species. Here we use genomic tools to investigate in Heliconius, a rapidly radiating genus neotropical butterflies widely used studies ecology, behaviour, mimicry speciation. We sequenced the genome Heliconius melpomene compared it with other taxa chromosomal evolution Lepidoptera gene flow among...
Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is major vector Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced genome R. prolixus, generated assembled sequences covering 95% (∼ 702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses this obligate blood-feeding insect. Although immune-deficiency (IMD)-mediated immune responses were observed,...
The tobacco cutworm, Spodoptera litura, is among the most widespread and destructive agricultural pests, feeding on over 100 crops throughout tropical subtropical Asia. By genome sequencing, physical mapping transcriptome analysis, we found that gene families encoding receptors for bitter or toxic substances detoxification enzymes, such as cytochrome P450, carboxylesterase glutathione-S-transferase, were massively expanded in this polyphagous species, enabling its extraordinary ability to...
Helicoverpa armigera and zea are major caterpillar pests of Old New World agriculture, respectively. Both, particularly H. armigera, extremely polyphagous, has developed resistance to many insecticides. Here we use comparative genomics, transcriptomics resequencing elucidate the genetic basis for their properties as pests.
Emergence of polyphagous herbivorous insects entails significant adaptation to recognize, detoxify and digest a variety host-plants. Despite its biological practical importance - since eat 20% crops no exhaustive analysis gene repertoires required for adaptations in generalist insect herbivores has previously been performed. The noctuid moth Spodoptera frugiperda ranks as one the world's worst agricultural pests. This is while majority other lepidopteran are specialist. It consists two...
Abstract Insects detect their hosts or mates primarily through olfaction, and olfactory receptors (ORs) are at the core of odorant detection. Each species has evolved a unique repertoire ORs whose functional properties expected to meet its ecological needs, though little is known about molecular basis olfaction outside Diptera. Here we report pioneer analysis large array in lepidopteran, herbivorous pest Spodoptera littoralis . We demonstrate that most narrowly tuned ubiquitous plant...
Sequences coding for chemosensory proteins (CSP) CSPMbraA and CSPMbraB, soluble of low mol. wt, have been amplified using polymerase chain reaction on antennal pheromonal gland complementary DNAs. On the basis their sequences, these could be classed in ‘OS-D like’ protein family whose first member was described Drosophila, that includes characterized organs many insect phylla, including our recent identification Mamestra brassicae proboscis. Binding assays shown bind component...
Secondary plant compounds are strong deterrents of insect oviposition and feeding, but may also be attractants for specialist herbivores. These insect-plant interactions mediated by gustatory receptors (Grs) olfactory (Ors). An analysis the reference genome butterfly Heliconius melpomene, which feeds on passion-flower vines (Passiflora spp.), together with whole-genome sequencing within species across phylogeny has permitted an unprecedented opportunity to study patterns gene duplication...
The codling moth, Cydia pomonella, is an important fruit pest worldwide. As nocturnal animals, adults depend to a large extent on olfactory cues for detection of food and mates, and, females, oviposition sites. In insects, odor mediated by odorant receptors (ORs) ionotropic (IRs), which ensure the specificity sensory neuron responses. this study, our aim was identify chemosensory in moth as means uncover new targets behavioral interference. Using next-generation sequencing techniques, we...
Abstract Background Nocturnal insects such as moths are ideal models to study the molecular bases of olfaction that they use, among examples, for detection mating partners and host plants. Knowing how an odour generates a neuronal signal in insect antennae is crucial understanding physiological olfaction, also could lead identification original targets development olfactory-based control strategies against herbivorous moth pests. Here, we describe Expressed Sequence Tag (EST) project...
Abstract Lepidoptera suffer critical lack of genetic tools and heritable genome edition has been achieved only in a few model species. Here we demonstrate that the CRISPR/Cas9 system is highly efficient for editing non-model crop pest Lepidoptera, noctuid moth Spodoptera littoralis . We knocked-out olfactory receptor co-receptor Orco gene to investigate its function olfaction. find 89.6% injected individuals carried mutations, 70% which transmitted them next generation. CRISPR/Cas9-mediated...
Abstract The codling moth Cydia pomonella , a major invasive pest of pome fruit, has spread around the globe in last half century. We generated chromosome-level scaffold assembly including Z chromosome and portion W chromosome. This reveals duplication an olfactory receptor gene ( OR3 ), which we demonstrate enhances ability C. to exploit kairomones pheromones locating both host plants mates. Genome-wide association studies contrasting insecticide-resistant susceptible strains identify...
To better understand the olfactory mechanisms in a lepidopteran pest model species, cotton leafworm Spodoptera littoralis, we have recently established partial transcriptome from adult antennae. Here, completed this using next generation sequencing technologies, namely 454 and Illumina, on both antennae larval tissues, including caterpillar maxillary palps. All sequences were assembled 77,643 contigs. Their analysis greatly enriched repertoire of chemosensory genes with total 57 candidate...
Plant volatiles mediate host discrimination and finding in phytophagous insects. Understanding how insects recognize these signals is a current challenge chemical ecology research. Pear ester, ethyl (E,Z)-2,4-decadienoate, powerful, bisexual attractant of codling moth Cydia pomonella (Lepidoptera, Tortricidae) strongly synergizes the male response to female-produced sex pheromone. We show here that odorant receptor (OR) CpomOR3 dedicated detecting this plant volatile. Heterologous expression...
Odorant receptors (ORs) are essential for plant-insect interactions. However, despite the global impacts of Lepidoptera (moths and butterflies) as major herbivores pollinators, little functional data available about ORs involved in plant-volatile detection. Here, we initially characterized plant-volatile-sensing function(s) 44 from cotton bollworm Helicoverpa armigera, subsequently conducted a large-scale comparative analysis that establishes how most orthologous have functionally diverged...
Sex pheromone receptors (PRs) are key players in chemical communication between mating partners insects. In the highly diversified insect order Lepidoptera, male PRs tuned to female-emitted type I pheromones (which make up vast majority of identified) form a dedicated subfamily odorant (ORs). Here, using combination heterologous expression and vivo genome editing methods, we bring functional evidence that at least one moth PR does not belong this but distantly related OR lineage. This PR,...
Ecosystems are brimming with myriad compounds, including some at very low concentrations that indispensable for insect survival and reproduction. Screening strategies identifying active compounds typically based on bioassay-guided approaches.
Previous biochemical evidence suggests that a cytochrome P450 specific to male antennae of the pale-brown chafer, Phyllopertha diversa , has evolved as pheromone-degrading enzyme. By using bioinformatics approach, we have now cloned three cDNAs: CYP4AW1, CYP4AW2, and CYP6AT1. RT-PCR indicated CYP4AW2 is expressed in all tissues examined, CYP6AT1 antennae-rich, CYP4AW1 antennae-specific. Both tissue specificity electrophysiological studies strongly support P. enzyme involved pheromone...
Recent studies have suggested that pheromone-degrading enzymes belonging to the carboxylesterase family could play a role in dynamics of olfactory response acetate sex pheromones insects. Bioinformatic analyses male antennal expressed sequence tag library allowed identification 19 putative esterase genes antennae moth Spodoptera littoralis. Phylogenetic analysis revealed these belong different insect clades, defined by their cellular localization and substrate preferences. Interestingly, two...
Abstract Moth sex pheromone communication is recognised as a long‐standing model for insect olfaction studies, and widespread knowledge has been accumulated on this subject thanks to numerous chemical, electrophysiological behavioural studies. A key step the identification of candidate receptors, opening new routes understanding specificity sensitivity system, but only few these receptors have yet functionally characterised. In context, we aim at unravelling molecular bases reception in...
A new family of candidate chemosensory ionotropic receptors (IRs) related to glutamate (iGluRs) was recently discovered in Drosophila melanogaster. Through Blast analyses an expressed sequenced tag library prepared from male antennae the noctuid moth Spodoptera littoralis, we identified 12 unigenes encoding proteins D. melanogaster and Bombyx mori IRs. Their full length sequences were obtained their expression patterns suggest that they exclusively or clearly enriched organs. The deduced...