A5050330316- Insect Resistance and Genetics
- Insect and Pesticide Research
- Insect-Plant Interactions and Control
- Insect Pest Control Strategies
- Insect symbiosis and bacterial influences
- Entomopathogenic Microorganisms in Pest Control
- Neurobiology and Insect Physiology Research
- Plant and animal studies
- Advanced Biosensing Techniques and Applications
- Plant Virus Research Studies
- Insect and Arachnid Ecology and Behavior
- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Genomics, phytochemicals, and oxidative stress
- French Urban and Social Studies
- Hemiptera Insect Studies
- Vector-borne infectious diseases
- Click Chemistry and Applications
- Environmental Toxicology and Ecotoxicology
- Computational Drug Discovery Methods
- Insect behavior and control techniques
- Horticultural and Viticultural Research
- Cognitive and psychological constructs research
- Glutathione Transferases and Polymorphisms
- Piperaceae Chemical and Biological Studies
Université Côte d'Azur
2011-2025
Centre National de la Recherche Scientifique
2013-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Assistance Publique – Hôpitaux de Paris
2025
Institut Cochin
2025
Institut Sophia Agrobiotech
2013-2024
Stago (France)
2022
Observatoire de la Côte d’Azur
2019
Laboratoire Ingénierie, Procédés, Aliments
2014-2016
AXO Science (France)
2015
Insecticide resistance is one of the most widespread genetic changes caused by human activity, but we still understand little about origins and spread resistant alleles in global populations insects. Here, via microarray analysis all P450s Drosophila melanogaster , show that DDT-R a gene conferring to DDT, associated with overtranscription single cytochrome P450 gene, Cyp6g1 . Transgenic shows this alone both necessary sufficient for resistance. Resistance up-regulation are allele has...
Insects use hydrocarbons as cuticular waterproofing agents and contact pheromones. Although their biosynthesis from fatty acyl precursors is well established, the last step of hydrocarbon long-chain aldehydes has remained mysterious. We show here that insects a P450 enzyme CYP4G family to oxidatively produce aldehydes. Oenocyte-directed RNAi knock-down Drosophila CYP4G1 or NADPH-cytochrome reductase results in flies deficient hydrocarbons, highly susceptible desiccation, with reduced...
Emergence of polyphagous herbivorous insects entails significant adaptation to recognize, detoxify and digest a variety host-plants. Despite its biological practical importance - since eat 20% crops no exhaustive analysis gene repertoires required for adaptations in generalist insect herbivores has previously been performed. The noctuid moth Spodoptera frugiperda ranks as one the world's worst agricultural pests. This is while majority other lepidopteran are specialist. It consists two...
Abstract S podoptera frugiperda is a polyphagous lepidopteran pest that encounters wide range of toxic plant metabolites in its diet. The ability this insect to adapt chemical environment might be explained by the action major detoxification enzymes such as cytochrome P450s (or CYP). Forty‐two sequences coding for were identified and most transcripts found expressed midgut, M alpighian tubules fat body . larvae. Relatively few established cell line Sf9 In order gain information on how these...
The P450 enzymes maintain a conserved fold despite considerable variation in sequence. family even includes proteins that lack the single cysteine and are therefore no longer haem-thiolate proteins. mechanisms of successive gene duplications leading to large families plants animals well established. Comparisons CYP clusters related species illustrate rapid changes CYPome sizes. Examples copy number with effects on fitness emerging, these provide an opportunity study proximal causes...
Understanding the genetic basis of insecticide resistance is a key topic in agricultural ecology. The adaptive evolution multi-copy detoxification genes has been interpreted as cause resistance, yet same pattern can also be generated by adaptation to host-plant defense toxins. In this study, we tested fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), if copy number variation caused two geographically distinct populations with different levels and strains. We observed significant...
Abstract Background Although native to North America, the invasion of aphid-like grape phylloxera Daktulosphaira vitifoliae across globe altered course cultivation. For past 150 years, viticulture relied on grafting-resistant American Vitis species as rootstocks, thereby limiting genetic stocks tolerant other stressors such pathogens and climate change. Limited understanding insect genetics resulted in successive outbreaks when rootstocks failed. Here we report 294-Mb genome D. a basic tool...
Abstract Endogenous viruses form an important proportion of eukaryote genomes and a source novel functions. How large DNA integrated into genome evolve when they confer benefit to their host, however, remains unknown. Bracoviruses are essential for the parasitism success parasitoid wasps, whose ~103 million years ago. Here we show, from assembly wasp at chromosomal scale, that bracovirus genes colonized all ten chromosomes Cotesia congregata . Most clusters involved in particle production or...
Abstract The Eyguières 42 strain of Drosophila simulans, obtained by laboratory selection, displayed ∼ 20 000‐fold resistance to the insecticide fipronil. Molecular cloning cDNA encoding RDL GABA receptor subunit this revealed presence two mutations: Rdl mutation (A301G) and an additional in third transmembrane domain (T350M). In order assess individual combined roles mutations fipronil resistance, functional properties wild‐type, A301G, T350M A301G/T350M homomultimeric receptors were...
Caffeine (1, 3, 7-trimethylxanthine), an alkaloid produced by plants, has antioxidant and insecticide properties that can affect metabolism cognition. In vertebrates, the metabolites derived from caffeine have been identified, their functions characterized. However, of in insects remain unknown. Thus, using radiolabelled caffeine, we identified some primary body Drosophila melanogaster males, including theobromine, paraxanthine theophylline. contrast to mammals, theobromine was predominant...
Lamins play a major role in the mechanical stability of cell nuclei, organisation chromatin and DNA replication, transcription repair. The expression profiles A-type B-type lamins vary depending on developmental stages, types tissues. Lamin B2 is expressed very early embryogenesis, especially central nervous system, where it essential for neuronal migration brain development. Pathogenic missense variants lamin have been linked to conditions such as lipodystrophy, progressive myoclonic...
Photorhabdus are Gram-negative, nematode-vectored bacteria that produce toxins to kill their insect hosts. The expression of one these, Makes caterpillars floppy 1 (Mcf1), is sufficient allow Escherichia coli survive within, and kill, which otherwise able clear E. infection. Mcf1 treated show rapid loss body turgor (the 'floppy' phenotype) death associated with massive apoptosis both the midgut epithelium phagocytes. Mammalian tissue culture cells also display key features including zeiosis,...
Abstract BACKGROUND The withdrawal of malathion in the European Union 2009 resulted a large increase lambda‐cyhalothrin applications for control Mediterranean fruit fly, Ceratitis capitata, Spanish citrus crops. RESULTS field populations C. capitata have developed resistance to (6–14‐fold), achieving LC 50 values (129–287 ppm) higher than recommended concentration treatments (125 ppm). These results contrast with high susceptibility found three Tunisian populations. We studied mechanism...
Abstract Essential oils are well known to act as biopesticides. This research evaluated the acute toxicity and synergistic effect of essential oil compounds in combination with cypermethrin against Spodoptera litura Fabricius (Lepidoptera: Noctuidae). The effects distillation extracts from Alpinia galanga Zingiberaceae (Zingiberales) rhizomes Ocimum basilicum Lamiaceae (Lamiales) leaves; one their primary 1,8-cineole; linalool were studied on second-instar S. by topical application under...