A5008210839- Nuclear Structure and Function
- RNA Research and Splicing
- Malaria Research and Control
- Traditional and Medicinal Uses of Annonaceae
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Berberine and alkaloids research
- Insect Pest Control Strategies
- Lipid metabolism and biosynthesis
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Natural product bioactivities and synthesis
- Genomics and Chromatin Dynamics
- Ethnobotanical and Medicinal Plants Studies
- Research in Cotton Cultivation
- Historical and Literary Studies
- Insect and Pesticide Research
- Cell Adhesion Molecules Research
- Phytochemistry and biological activities of Ficus species
- Agriculture and Rural Development Research
- Essential Oils and Antimicrobial Activity
- RNA and protein synthesis mechanisms
- Cancer therapeutics and mechanisms
- Phase Equilibria and Thermodynamics
- DNA Repair Mechanisms
Aix-Marseille Université
2013-2025
Inserm
2016-2025
Centre de Génétique Médicale de Marseille
2023-2024
Génétique Médicale & Génomique Fonctionelle
2016-2023
Laboratoire de Chimie
2016-2021
Génétique et Immunologie des Maladies Parasitaires
2016
Institut de Médecine Tropicale du Service de Santé des Armées
2013
Institut de Neurobiologie de la Méditerranée
2012
Lamins play a major role in the mechanical stability of cell nuclei, organisation chromatin and DNA replication, transcription repair. The expression profiles A-type B-type lamins vary depending on developmental stages, types tissues. Lamin B2 is expressed very early embryogenesis, especially central nervous system, where it essential for neuronal migration brain development. Pathogenic missense variants lamin have been linked to conditions such as lipodystrophy, progressive myoclonic...
ZMPSTE24 encodes the only metalloprotease, which transforms prelamin into mature lamin A. Up to now, mutations in have been linked Restrictive Dermopathy (RD), Progeria or Mandibulo-Acral Dysplasia (MAD). We report here phenotype of a patient referred for severe metabolic syndrome and cardiomyopathy, carrying mutation ZMPSTE24. The presented with partial lipodystrophic associating hypertriglyceridemia, early onset type 2 diabetes, android obesity truncal abdominal fat accumulation but...
A better anti-malarial efficiency and lower neurotoxicity have been reported for mefloquine (MQ) (+)- enantiomer. However, the importance of stereoselectivity remains poorly understood as activity pure enantiomer MQ analogues has never described. Building on these observations, a series enantiopure 4-aminoalcohol quinoline derivatives previously synthesized to optimize reduce possible adverse effects. Their in vitro Plasmodium falciparum W2 3D7 strains is here along with their inhibition...
Stephania rotunda is used by traditional health practitioners in Southeast Asia to treat a wide range of diseases and particularly symptoms related malaria. Cepharanthine (CEP) an alkaloid isolated from this plant with potential innovative antiplasmodial activity. The analysis interactions between drugs necessary develop new combinations prevent de novo emergence resistance. objective study was evaluate the anti-malarial activity CEP combination usual compounds, both vitro vivo.A fixed ratio...
Although 100% of untreated mice infected with Plasmodium berghei died specific signs cerebral malaria and treated 3 mg/kg dihydroartemisinin, the active metabolite artesunate, which is used as first-line treatment for severe malaria, also but showed no 78% 10 Proveblue (methylene blue) a combination mg dihydroartemisinin survived or detectable parasites.
Four series of carbazole derivatives, including N-substituted-hydroxycarbazoles, oxazinocarbazoles, isoxazolocarbazolequinones, and pyridocarbazolequinones, were studied using diverse biological test methods such as a CE-based assay for CK2 activity measurement, cytotoxicity with IPC-81 cell line, determination MIC derivatives antibacterial agents, Plasmodium falciparum susceptibility assay, an ABCG2-mediated mitoxantrone assay. Two oxazinocarbazoles Ib Ig showed inhibition IC50 = 8.7 14.0...
Abstract Background The medical care of malaria is a clinical emergency because it may develop into severe malaria, which has high risk complications and death. One the major Plasmodium falciparum infections cerebral (CM), responsible for at least 175,000 deaths worldwide each year long-term neurological sequelae. Moreover, treatment CM only partially effective. Statins are now known to have anti-inflammatory action, attenuate sepsis neuroprotective effects. In vitro, atorvastatin (AVA) an...
Abstract Background Proveblue®, a methylene blue dye that complies with European Pharmacopoeia and contains limited organic impurities heavy metals of recognized toxicity, showed in vitro synergy against Plasmodium falciparum when combined atorvastatin, an inhibitor 3-hydroxy-3-methylglutaryl-Coenzyme A reductase. The objective this study was to evaluate the vivo efficacy Proveblue® atorvastatin murine model experimental cerebral malaria. Methods Forty female C57Bl6/N mice were divided into...
This study details the clinical and cellular phenotypes associated with two missense heterozygous mutations in LMNA, c.1745G>T p.(Arg582Leu), c.1892G>A p.(Gly631Asp), patients early onset of diabetes mellitus, hypertriglyceridemia non-alcoholic fatty liver disease. In these patients, subcutaneous adipose tissue was persistent, at least on abdomen, serum leptin level remained normal range. Cellular studies showed elevated nuclear anomalies, an accelerated senescence rate a decrease...
The spread of Plasmodium falciparum resistance toward most the used drugs requires new antimalarial compounds. Taking advantage biodiversity, ethnopharmacological approach opens way for discovery and characterization potent original molecules. Previous works led to selection a bisbenzylisoquinoline, cepharanthine, extracted from Stephania rotunda , which is mainly present in Cambodia. A sensitive selective liquid chromatography method has been developed determination cepharanthine mouse...
Lamin A/C is a well-established key contributor to nuclear stiffness and its role in nucleus mechanical properties has been extensively studied. However, impact on whole-cell mechanics poorly addressed, particularly concerning measurable physical parameters. In this study, we combined microfluidic experiments with theoretical analyses quantitatively estimate the properties. This allowed us characterize changes induced cells by lamin alterations prelamin A accumulation resulting from...
A study of the behavioral responses Aedes aegypti and Anopheles minimus to 3 Cambodian plant extracts at different concentrations (1%, 2.5%, 5%) was performed using an excito-repellency test system. These plants were Strophanthus scandens, Capparis micracantha, Dioscorea hispida, selected according traditional healer's knowledge, bibliographic studies market surveys. Results showed that S. scandens leaves' hexane extract only one exert repellency against Ae. with 23.3% escaped mosquitoes a...
Many proteins are causative for inherited partial lipodystrophies, including lamins, the essential constituents of nuclear envelope scaffold called lamina. By performing high throughput sequencing on a panel genes involved in we identified heterozygous mutation LMNB2 gene (c.700C > T p.(Arg234Trp)) female patient presenting early onset type II diabetes, hypertriglyceridemia, and android fat distribution. This is rare general population (frequency 0.013% GnomAD) was predicted pathogenic by...
Familial partial lipodystrophy type 2 is an autosomal dominant disorder generally due to heterozygous missense variants in the LMNA gene.In women, phenotype begins manifest before puberty, while men onset later.It striking that most of cases reported literature are women.Specifically, our cohort only 27% these patients were men.Considering pattern inheritance disease where a similar ratio between males and females would be expected, it obvious majority with this do not they diagnosed.The...
Abstract Lamin A/C is a well-established key contributor to nuclear stiffness and its role in nucleus mechanical properties has been extensively studied. However, impact on whole cell mechanics poorly addressed, even less so terms of measurable physical parameters. In the present study, microfluidic experiments combined with theoretical analyses were performed provide quantitative estimation properties. This allowed characterization changes induced by lamin alterations resulting from...