A5021413003- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Orthoptera Research and Taxonomy
- Phytoplasmas and Hemiptera pathogens
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Vectors
- Neurobiology and Insect Physiology Research
- Insect Pest Control Strategies
- Hymenoptera taxonomy and phylogeny
- Insect and Arachnid Ecology and Behavior
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Invertebrate Taxonomy and Ecology
- Oil Palm Production and Sustainability
- Invertebrate Immune Response Mechanisms
- Plant and animal studies
- Cholinesterase and Neurodegenerative Diseases
- Pharmaceutical and Antibiotic Environmental Impacts
- Plant Disease Resistance and Genetics
- Mosquito-borne diseases and control
- Plant Pathogenic Bacteria Studies
- Biosensors and Analytical Detection
China Jiliang University
2020-2025
Zhejiang University
2015-2023
State Key Laboratory of Rice Biology
2019-2023
Ministry of Agriculture and Rural Affairs
2020-2023
Northeast Forestry University
2021-2022
Nanjing Agricultural University
2014-2016
The genomes and transcriptomes of hundreds insects have been sequenced. However, insect community lacks an integrated, up-to-date collection gene data. Here, we introduce the first release InsectBase, available online at http://www.insect-genome.com. database encompasses 138 genomes, 116 transcriptomes, 61 sets, 36 families 60 insects, 7544 miRNAs 69 96 925 piRNAs Drosophila melanogaster Chilo suppressalis, 2439 lncRNA Nilaparvata lugens, 22 536 pathways 78 678 881 untranslated regions (UTR)...
Abstract The codling moth Cydia pomonella , a major invasive pest of pome fruit, has spread around the globe in last half century. We generated chromosome-level scaffold assembly including Z chromosome and portion W chromosome. This reveals duplication an olfactory receptor gene ( OR3 ), which we demonstrate enhances ability C. to exploit kairomones pheromones locating both host plants mates. Genome-wide association studies contrasting insecticide-resistant susceptible strains identify...
The functional repertoire of long noncoding RNA (lncRNA) has been characterized in several model organisms, demonstrating that lncRNA plays important roles fundamental biological processes. However, they remain largely unidentified most species. Understanding the characteristics and functions insects would be useful for insect resources utilization sustainable pest control. A computational pipeline was developed to identify genes rice brown planthopper, Nilaparvata lugens, a destructive...
Parasitic wasps produce several factors including venom, polydnaviruses (PDVs) and specialized wasp cells named teratocytes that benefit the survival of offspring by altering physiology hosts. However, underlying molecular mechanisms for alterations remain unclear. Here we find Cotesia vestalis, an endoparasitoid diamondback moth Plutella xylostella, its associated bracovirus (CvBV) can miRNAs deliver products into host via different ways. Certain in parasitized are mainly produced...
The rice stem borer, Chilo suppressalis, is one of the most damaging insect pests to production worldwide. Although C. suppressalis has been focus numerous studies examining cold tolerance and diapause, plant-insect interactions, pesticide targets resistance, development RNAi-mediated pest management, absence a high-quality genome limited deeper insights. To address this limitation, we generated draft constructed from both Illumina PacBio sequences. assembled size was 824.35 Mb with contig...
Parasitoid wasps are well-known natural enemies of major agricultural pests and arthropod borne diseases. The parasitoid wasp Macrocentrus cingulum (Hymenoptera: Braconidae) has been widely used to control the notorious insect Ostrinia furnacalis (Asian Corn Borer) O. nubilalis (European corn borer). One striking phenomenon exhibited by M. is polyembryony, formation multiple genetically identical offspring from a single zygote. Moreover, employs passive parasitic strategy preventing host's...
Significance Adaptation to more severe ambient temperature fluctuations can be considered one of the key innovations terrestrial tetrapods. Our study shows formation functional MHR1-3 domain in transient receptor potential melastatin 8 (TRPM8) bestowed channel with cold sensitivity during water-to-land transition. The evolved found tetrapods serves as an independent apparatus sensitivity. Furthermore, this is necessary for regulatory mechanism pore domain, where efficacy activation largely...
Ciprofloxacin has been extensively utilized in aquaculture due to its remarkable efficacy preventing and treating bacterial infections fish animals. However, the widespread application of ciprofloxacin led significant residue accumulation, necessitating development rapid, sensitive specific detection methods. In this study, we developed a novel dual-mode quantitative immunochromatographic assay based on portable reader photothermal instrument, enabling on-site detection. Under optimized...
Insecticide resistance is a substantial problem in controlling agricultural and medical pests. Detecting target site mutations crucial to manage insecticide resistance. Though PCR-based methods have been widely used this field, they are time-consuming inefficient, typically high false positive rate. Acetylcholinesterases (Ace) the neural of organophosphate (OP) carbamate insecticides. However, there not any software available detect associated RNA-Seq data at present.A computational pipeline...
Abstract Background A fundamental feature of parasitism is the nutritional exploitation host organisms by their parasites. Parasitoid wasps lay eggs on arthropod hosts, exploiting them for nutrition to support larval development using diverse effectors aimed at regulating metabolism. However, genetic components and molecular mechanisms basis such exploitation, especially utilization amino acid resources, remain largely unknown. To address this question, here, we present a chromosome-level...
Abstract Background Parasitic insects are well-known biological control agents for arthropod pests worldwide. They capable of regulating their host’s physiology, development and behaviour. However, many the molecular mechanisms involved in host-parasitoid interaction remain unknown. Results We sequenced genomes two parasitic wasps ( Cotesia vestalis , Diadromus collaris ) that parasitize diamondback moth Plutella xylostella using Illumina Pacbio sequencing platforms. Genome assembly...
Abstract Some endoparasitoid wasps lay eggs that produce cells called teratocytes. In this study, we sequenced and analyzed the transcriptome of teratocytes from solitary Cotesia vestalis (Braconidae), which parasitizes larval stage Plutella xylostella (Plutellidae). Results identified many teratocyte transcripts with potential functions in affecting host immune defenses, growth or metabolism. Characterization teratocyte-secreted venom-like protein 8 (TSVP-8) indicated it inhibits...
Abstract Mealybugs (Hemiptera: Pseudococcidae) are economically important agricultural pests with several compelling biological phenomena including paternal genome elimination (PGE). However, limited high‐quality assemblies of mealybugs hinder a full understanding this striking and unusual phenomenon. Here, we generated chromosome‐level assembly cotton mealybug, Phenacoccus solenopsis , by combining Illumina short reads, PacBio long reads Hi‐C scaffolding. The assembled was 292.54 Mb contig...
Abstract BACKGROUND Apple snails from the genus Pomacea have spread widely in paddy fields and other wetlands of southern China since their introduction 1980s. spp. are commonly identified using mitochondrial COI sequences. However, sequencing nuclear elongation factor 1‐alpha (EF1α) gene revealed genetic introgression between field populations P. canaliculata maculata , which produce surviving hybrids laboratory crossbreeding experiments. RESULTS In this study, we sequenced 1054 EF1α clones...
Insects are one of the most successful animal groups on earth. Some insects, such as silkworm and honeybee, beneficial to humans, whereas others notorious pests crops. At present, genomes 38 insects have been sequenced made publically available. In addition, transcriptomes dozens sequenced. As gene data rapidly accumulate, constructing pathway molecular interactions becomes increasingly important for entomological research. Here, we developed an improved tool, iPathCons, knowledge-based...
A recent breakthrough in agricultural biotechnology is the introduction of RNAi-mediated strategies pest control. However, off-target effects RNAi control are still not fully understood. Here, we studied two insecticidal siRNAs both target and non-target insects. The results revealed that occur widely We classified expression-changed genes according to their homology siRNA-targeted gene, related KEGG pathways with gene continuous matches siRNAs. Surprisingly, unintended significant changes...
Abstract Nipa palm hispid beetle ( Octodonta nipae ) is an insect species that native to Malaysia but has spread southern China and beyond, seriously threatening production. A lack of high-quality genome resources hindered understanding the insect’s invasive characteristics ecological adaptations. Here, we combined Illumina short read, PacBio long-read, high-throughput chromosome conformation capture (Hi-C) sequencing technologies generate a high-quality, chromosome-scale assembly nipa...
Tomato leaf curl New Delhi virus (ToLCNDV), a member of the genus Begomovirus in family Geminiviridae is naturally transmitted by whitefly Bemisia tabaci (order Hemiptera, Aleyrodidae) circulative and persistent manner (Moriones et al. 2017). ToLCNDV has occurred Bangladesh, India, Indonesia, Iran, Italy, Malaysia, Pakistan, Sri Lanka, Spain, Thailand Tunisia To date, The primary cultivated host been identified as tomato (Lycopersicon esculentum), but also known to infect 43 other plant...
Abstract Sequencing and assembling a genome with single individual have several advantages, such as lower heterozygosity easier sample preparation. However, the amount of genomic DNA some small sized organisms might not meet standard input requirement for current sequencing pipelines. Although few studies sequenced insect about 100 ng input, it may still be challenging many to obtain from individual. Here, we use 20 present high-quality assembly haploid male parasitoid wasp ( Habrobracon...
HomePlant DiseaseVol. 107, No. 5First Report of Tomato Yellow Mottle-Associated Virus Infecting Pepper (Capsicum annuum) in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst ChinaChangjie Li, Yi Liu, Chuanlin Yin, Kai Sun, Xiaoping Yu, Guangfu and Pengjun ZhangChangjie LiZhejiang Provincial Key Laboratory Biometrology Inspection & Quarantine, College Life Sciences, Jiliang University, Hangzhou 310018, Zhejiang Province, People's Republic China, LiuZhejiang YinZhejiang...
Ulomoides dermestoides, a member of the Tenebrionidae family, is known by various common names such as Chinese beetle, weevil, peanut cancer or asthma beetle. Despite its origin in Asia, it has achieved global distribution owing to perceived medicinal properties. In this study, we employed combination Illumina short-read, PacBio long-read, and high-throughput chromosome conformation capture (Hi-C) sequencing technologies produce high-quality, chromosome-scale genome assembly U. dermestoides....