A5007670130- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Ion Channels and Receptors
- Venomous Animal Envenomation and Studies
- Antimicrobial Peptides and Activities
- Physiological and biochemical adaptations
- Nicotinic Acetylcholine Receptors Study
- Insect and Pesticide Research
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Pain Mechanisms and Treatments
- Biochemical and Structural Characterization
- Essential Oils and Antimicrobial Activity
- Animal Behavior and Reproduction
- High-Temperature Coating Behaviors
- Healthcare and Venom Research
- Immunotherapy and Immune Responses
- Bioactive Compounds and Antitumor Agents
- Hematological disorders and diagnostics
- Plant Parasitism and Resistance
- Acute Myeloid Leukemia Research
- Silk-based biomaterials and applications
- Thermoregulation and physiological responses
- Cancer Cells and Metastasis
- Circadian rhythm and melatonin
Northeast Forestry University
2020-2025
University of Chinese Academy of Sciences
2011-2024
East China University of Science and Technology
2024
Chongqing University of Posts and Telecommunications
2023
Rutgers, The State University of New Jersey
2022
Kunming Institute of Zoology
2012-2021
Chinese Academy of Sciences
2012-2021
Children's Hospital of Zhejiang University
2014
National Engineering Research Center of Human Stem Cells
2008
Hangzhou Children's Hospital
2004
Significance The economic burden of chronic pain in the United States is currently ∼$600 billion per annum, which exceeds combined annual cost cancer, heart disease, and diabetes. Few drugs are available for treating pain, many have limited efficacy dose-limiting side-effects. Humans with inheritable loss-of-function mutations voltage-gated sodium channel Na V 1.7 indifferent to all types therefore that block this should be useful analgesics conditions. Herein we describe Ssm6a, a peptide...
Centipedes are excellent predatory arthropods that inject venom to kill or immobilize their prey. Although centipedes have long been known be venomous, venoms remain largely unexplored. The chemical components responsible for centipede predation and the functional mechanisms unknown. Twenty-six neurotoxin-like peptides belonging ten groups were identified from venoms, Scolopendra subspinipes mutilans L. Koch by peptidomics combined with transcriptome analysis, revealing diversity of...
Abstract The capsaicin receptor TRPV1 ion channel is a polymodal nociceptor that responds to heat with exquisite sensitivity through an unknown mechanism. Here we report the identification of novel toxin, RhTx, from venom Chinese red-headed centipede potently activates produce excruciating pain. RhTx 27-amino-acid small peptide forms compact polarized molecule very rapid binding kinetics and high affinity for TRPV1. We show targets channel’s activation machinery cause powerful at body...
Wound-healing represents a major health burden, such as diabetes-induced skin ulcers and burning. Many works are being tried to find ideal clinical wound-healing biomaterials. Especially, small molecules with low cost function promote production of endogenous wound healing agents (i.e. transforming growth factor beta, TGF-β) excellent candidates. In this study, peptide (tiger17, c[WCKPKPKPRCH-NH2]) containing only 11 amino acid residues was designed proved be potent healer. It showed strong...
Abstract Menthol in mints elicits coolness sensation by selectively activating TRPM8 channel. Although structures of were determined the apo and liganded states, menthol-bounded state is unresolved. To understand how menthol activates channel, we docked to channel systematically validated our binding models with thermodynamic mutant cycle analysis. We observed that uses its hydroxyl group as a hand specifically grab R842, isopropyl legs stand on I846 L843. By imaging fluorescent unnatural...
Abstract The transient receptor potential vanilloid 1 (TRPV1) ion channel is a prototypical molecular sensor for noxious heat in mammals. Its role sustained response remains poorly understood, because rapid heat-induced desensitization (Dh) follows tightly activation (Ah). To understand the physiological and structural basis of Dh, we carried out comparative study TRPV1 channels mouse (mV1) those platypus (pV1), which naturally lacks Dh. Here show that temperature-sensitive interaction...
Centipedes can subdue giant prey by using venom, which is metabolically expensive to synthesize and thus used frugally through efficiently disrupting essential physiological systems. Here, we show that a centipede ( Scolopendra subspinipes mutilans , ∼3 g) mouse (∼45 within 30 seconds. We found this observation largely due peptide toxin in the SsTx, further established SsTx blocks KCNQ potassium channels exert lethal toxicity. also demonstrated opener, retigabine, neutralizes toxicity of...
To adapt to habitat temperature, vertebrates have developed sophisticated physiological and ecological mechanisms through evolution. Transient receptor potential melastatin 8 (TRPM8) serves as the primary sensor for cold. However, how cold activates TRPM8 this is tuned thermal adaptation remain largely unknown. Here we established a molecular framework of sensed in with combination patch-clamp recording, unnatural amino acid imaging, structural modeling. We first observed that maximum...
Although alkaline sensation is critical for survival, alkali-activated receptors are yet to be identified in vertebrates. Here, we showed that the OTOP1 channel can directly activated by extracellular alkali. Notably, biphasically mediated proton influx and efflux with acid base stimulation, respectively. Mutations of K221 R554 at S5-S6 S11-S12 linkers significantly reduced alkali affinity without affecting activation, suggesting different domains responsible acid- alkali-activation OTOP1....
Short QT syndrome (SQT) is a rare but arrhythmogenic disorder featured by shortened ventricular repolarization and propensity toward life-threatening arrhythmias sudden cardiac death.This study aimed to investigate the single-cell mechanism of SQT using patient-specific gene-corrected induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs).One patient carrying missense mutation T618I in potassium voltage-gated channel subfamily H member 2 ( KCNH2) was recruited as well healthy...
The intense pain induced by scorpion sting is a frequent clinical manifestation. To date, there no established protocol with significant efficacy to alleviate the envenomation. One of important reasons that, little information on pain-inducing compound from venoms available. Here, peptide (BmP01) has been identified and characterized (Mesobuthus martensii). In an animal model, intraplantar injection BmP01 in mouse hind paw showed acute wild type (WT) mice but not TRPV1 knock-out (TRPV1 KO)...
Significance Adaptation to more severe ambient temperature fluctuations can be considered one of the key innovations terrestrial tetrapods. Our study shows formation functional MHR1-3 domain in transient receptor potential melastatin 8 (TRPM8) bestowed channel with cold sensitivity during water-to-land transition. The evolved found tetrapods serves as an independent apparatus sensitivity. Furthermore, this is necessary for regulatory mechanism pore domain, where efficacy activation largely...
Organisms from cyanobacteria to humans have evolved a wide array of photoreceptive strategies detect light. Sunlight avoidance behavior is common in animals without vision or known photosensory genes. While indirect light perception via photothermal conversion possible scenario, there no experimental evidence for this hypothesis. Here, we show nonvisual and extraocular sunlight detection mechanism by identifying the broad-range thermal receptor 1 (BRTNaC1, temperature range = 33 48 °C)...
The development of fluorescent probes for the detection nerve agents has been a significant focus research due to their lethal toxicity humans. Inspired by excited state properties thermally activated delayed fluorescence (TADF), we designed two visualized probes, PT and PPT, that exhibit characteristics aggregation-induced emission. These are intended rapid highly sensitive diethyl chlorophosphate (DCP). Upon exposure DCP vapors, PPT demonstrated quenching in less than 5 s, which was...
We suggest three steps to test the hypothesis that transient receptor potential channels may respond temperature changes, increasing intracellular calcium concentration and triggering phosphorylation of STAT3, which subsequently regulates male female sex determination pathways in temperature-dependent species.
A scorpion toxin uses protons in the venom as coactivators to target nociceptive ion channel TRPV1 and cause pain.
Antimicrobial peptides have been widely identified from amphibian skins except salamanders. A novel antimicrobial peptide (CFBD) was isolated and characterized skin secretions of the salamander, Cynops fudingensis. The cDNA encoding CFBD precursor cloned library C. composed three domains: signal 17 residues, mature 41 residues intervening propeptide 3 residues. There are six cysteines in sequence peptide, which possibly form disulfide-bridges. showed activities against Staphylococcus aureus,...