A5045713877- Antimicrobial Peptides and Activities
- Neurobiology and Insect Physiology Research
- Venomous Animal Envenomation and Studies
- Ion channel regulation and function
- COVID-19 Clinical Research Studies
- Healthcare and Venom Research
- Phagocytosis and Immune Regulation
- SARS-CoV-2 and COVID-19 Research
- Amphibian and Reptile Biology
- Ion Channels and Receptors
- Essential Oils and Antimicrobial Activity
- Bee Products Chemical Analysis
- Food Allergy and Anaphylaxis Research
- Computational Drug Discovery Methods
- Stress Responses and Cortisol
- Dietary Effects on Health
- Meat and Animal Product Quality
- S100 Proteins and Annexins
- Yersinia bacterium, plague, ectoparasites research
- Vector-Borne Animal Diseases
- Bioactive Natural Diterpenoids Research
- Connexins and lens biology
- Herbal Medicine Research Studies
- Entomological Studies and Ecology
- Hemoglobin structure and function
Kunming Institute of Zoology
2019-2025
Chinese Academy of Sciences
2019-2025
University of Chinese Academy of Sciences
2019-2024
University of Hong Kong
2020-2024
Chinese University of Hong Kong
2022-2024
University of Nairobi
2023
Jomo Kenyatta University of Agriculture and Technology
2023
Hefei National Center for Physical Sciences at Nanoscale
2020
University of Science and Technology of China
2020
Chongqing Public Health Medical Center
2020
Abstract Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic worldwide. Currently, however, no effective drug or vaccine is available to treat prevent the resulting disease 2019 (COVID-19). Here, we report our discovery of promising anti-COVID-19 candidate, lipoglycopeptide antibiotic dalbavancin, based on virtual screening FDA-approved peptide library combined in vitro and vivo functional antiviral assays. Our results showed that dalbavancin...
Studies have shown significantly increased thromboembolic events at high altitude. We recently reported that transferrin could potentiate blood coagulation, but the underlying mechanism for altitude-related thromboembolism is still poorly understood. Here, we examined activity and concentration of plasma coagulation factors in collected from long-term human residents short-stay mice exposed to varying altitudes. found activities thrombin factor XIIa (FXIIa) along with concentrations were...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been detected in almost all organs of disease-19 patients, although some do not express angiotensin-converting enzyme-2 (ACE2), a known receptor SARS-CoV-2, implying the presence alternative receptors and/or co-receptors. Here, we show that ubiquitously distributed human transferrin (TfR), which binds to diferric traffic between membrane and endosome for iron delivery cycle, can ACE2-independently mediate SARS-CoV-2...
Abstract The transient receptor potential vanilloid 1 (TRPV1) ion channel is a prototypical molecular sensor for noxious heat in mammals. Its role sustained response remains poorly understood, because rapid heat-induced desensitization (Dh) follows tightly activation (Ah). To understand the physiological and structural basis of Dh, we carried out comparative study TRPV1 channels mouse (mV1) those platypus (pV1), which naturally lacks Dh. Here show that temperature-sensitive interaction...
Although alkaline sensation is critical for survival, alkali-activated receptors are yet to be identified in vertebrates. Here, we showed that the OTOP1 channel can directly activated by extracellular alkali. Notably, biphasically mediated proton influx and efflux with acid base stimulation, respectively. Mutations of K221 R554 at S5-S6 S11-S12 linkers significantly reduced alkali affinity without affecting activation, suggesting different domains responsible acid- alkali-activation OTOP1....
Abstract Angiotensin-converting enzyme 2 (ACE2) has been suggested as a receptor for severe acute respiratory syndrome coronavirus (SARS-CoV-2) entry to cause disease 2019 (COVID-19). However, no ACE2 inhibitors have shown definite beneficiaries COVID-19 patients, applying the presence of another SARS-CoV-2 entry. Here we show that knockout dose not completely block virus entry, while TfR directly interacts with Spike protein mediate and can infect mice over-expressed humanized transferrin...
Itching is the most frequent pathology in dermatology that has significant impacts on people's mental health and social life. Transient receptor potential vanilloid 3 (TRPV3) channel a promising target for treating pruritus. However, few selecetive potent antagonists have been reported. This study was designed to identify selective TRPV3 antagonist elucidate its anti-pruritus pharmacology.FlexStation calcium fluorescence imaging were conducted track functional compounds. Whole-cell patch...
Abstract Screening bioactive compounds from natural sources, including animals and plants, is a valuable strategy for identifying novel anti-tumor agents. α-Toxin BmK-M9, key component of scorpion venom, has received limited attention regarding its potential anti-cancer effects underlying mechanisms in breast cancer. This study investigates the BmK-M9 cancer using vitro experiments nude mouse model. mRNA sequencing was performed to identify affected signaling pathways, while Western blotting...
Antibiotic resistance is a global threat. Antimicrobial peptides (AMPs) are highly desirable to treat multidrug-resistant pathogen infection. However, few AMPs clinically available, due high cost, instability, and poor selectivity. Here, ultrashort (2–3 residues with an N-terminal cysteine) designed assembled as gold nanoparticles. Au–S conjugation size restrict nonspecific reactions peptide orientation, thus concentrating positively charged on the surface. The nanostructured assemblies...
Coumarins, naturally occurring phytochemicals, display a wide spectrum of biological activities by acting on multiple targets. Herein, nine coumarins from the root Toddalia asiatica were evaluated for related to pathogenesis Alzheimer's disease (AD). They examined acetylcholinesterase (AChE) and AChE- or self-induced amyloid beta (Aβ) aggregation inhibitory activities, as well neuroprotection against H2O2- Aβ1-42-induced human neuroblastoma SH-SY5Y cell damage. Moreover, in order understand...
Unlike healthy, non-transformed cells, the proteostasis network of cancer cells is taxed to produce proteins involved in tumor development. Cancer have a higher dependency on molecular chaperones maintain proteostasis. The chaperonin T-complex protein ring complex (TRiC) contains eight paralogous subunits (CCT1-8), and assists folding as many 10% cytosolic proteome. TRiC essential for progression some cancers, but roles osteosarcoma remain be explored. Here, we show that CCT4/TRiC...
Abstract Angiotensin-converting enzyme 2 (ACE2) has been suggested as a receptor for severe acute respiratory syndrome coronavirus (SARS-CoV-2) entry to cause disease 2019 (COVID-19). However, no ACE2 inhibitors have shown definite beneficiaries COVID-19 patients, applying the presence of another SARS-CoV-2 entry. Here we show that knockout dose not completely block virus entry, while TfR directly interacts with Spike protein mediate and can infect mice over-expressed humanized transferrin...
Accidental contact with caterpillar bristles causes local symptoms such as severe pain, intense heat, edema, erythema, and pruritus. However, there is little functional evidence to indicate a potential mechanism. In this study, we analyzed the biological characteristics of crude venom from larval stage Latoia consocia living in South-West China. Intraplantar injection into hind paws mice induced acute pain behaviors wild type (WT) mice; responses were much reduced TRPV1-deficit (TRPV1 KO)...
Prokineticins are highly conserved small peptides family expressed in all vertebrates, which contain a wide spectrum of functions. In this study, prokineticin homolog (Bv8-AJ) isolated from the venom frog Amolops jingdongensis was fully characterized. Bv8-AJ accelerated full-thickness wounds healing mice model by promoting initiation and termination inflammatory phase. Moreover, exerted strong proliferative effect on fibroblasts keratinocytes newborn activating interleukin (IL)-1 production....
African swine fever virus (ASFV) is a large double-stranded DNA and causes high mortality in swine. ASFV can be transmitted by biological vectors, including soft ticks genus Ornithodoros but not hard ticks. However, the underlying mechanisms evolved vectorial capacity of are well-understood. Here, we found that defensin-like peptide toxin OPTX-1 identified from papillipes inhibits enzyme activity pS273R protease with K i =0.821±0.526μM shows inhibitory on replication ASFV. The analogs show...
SignificanceAn immunosuppressant protein (MTX), which facilitates virus infection by inhibiting leukotriene A4 hydrolase (LTA4H) to produce the lipid chemoattractant B4 (LTB4), was identified and characterized from submandibular salivary glands of bat Myotis pilosus. To best our knowledge, this is a report an endogenous LTA4H inhibitor in animals. MTX highly concentrated glands, suggesting mechanism for generation immunological privilege immune tolerance providing evidence viral shedding...
Pain is the most common presenting physical symptom and a primary reason for seeking medical care, which chronically affects people's mental health social life. CaV3.2 channel plays an essential role in peripheral processing maintenance of pain states. This study was designed to identify novel drug candidates targeting channel. Whole-cell patch-clamp, cellular thermal shift assay, FlexStation, vivo vitro knock-down, site-directed mutagenesis, double-mutant cycle analysis were employed...