A5017754147- Insect symbiosis and bacterial influences
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Probiotics and Fermented Foods
- Gut microbiota and health
- Insect and Pesticide Research
- Microbial Metabolism and Applications
- Escherichia coli research studies
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Clostridium difficile and Clostridium perfringens research
- Insect behavior and control techniques
- Plant and fungal interactions
- Ruminant Nutrition and Digestive Physiology
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Salmonella and Campylobacter epidemiology
- Insect Utilization and Effects
- Milk Quality and Mastitis in Dairy Cows
- Blood donation and transfusion practices
- Agriculture Sustainability and Environmental Impact
- Animal health and immunology
- Evolutionary Game Theory and Cooperation
- Animal and Plant Science Education
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
University of Copenhagen
2012-2024
Microbiologie Environnement Digestif Santé
2020-2024
Génétique, Diversité, Écophysiologie des Céréales
2023-2024
Université Clermont Auvergne
2020-2023
IT University of Copenhagen
2022
Université Claude Bernard Lyon 1
2013-2014
Institut National des Sciences Appliquées de Lyon
2014
Institut National de la Recherche Agronomique
2013
University of Ioannina
2007-2011
The obligate intracellular bacterium Wolbachia pipientis infects around 20% of all insect species. It is maternally inherited and induces reproductive alterations populations by male killing, feminization, parthenogenesis, or cytoplasmic incompatibility. Here, we present the 1,445,873-bp genome W. strain wRi that very strong incompatibility in its natural host Drosophila simulans. A comparison with previously sequenced wMel from melanogaster identified 35 breakpoints associated mobile...
The ancient fungus-growing termite (Mactrotermitinae) symbiosis involves the obligate association between a lineage of higher termites and basidiomycete Termitomyces cultivar fungi. Our investigation Macrotermes natalensis shows that Bacillus strains from M. colonies produce single major antibiotic, bacillaene A (1), which selectively inhibits known putatively antagonistic fungi Termitomyces. Comparative analyses genomes symbiotic revealed they are phylogenetically closely related to...
ABSTRACT Following cultivation-dependent and -independent techniques, we investigated the microbiota associated with Bactrocera oleae , one of major agricultural pests in olive-producing countries. Bacterial 16S rRNA gene libraries ultrastructural analyses revealed presence several bacterial taxa this insect, among which Acetobacter tropicalis was predominant. The recent increased detection acetic acid bacteria as symbionts other insect model organisms, such Anopheles stephensi (G. Favia et...
Ecological and evolutionary theories predict that parasitism mutualism are not fixed endpoints of the symbiotic spectrum. Rather, may be host or environment dependent, induced by same genetic machinery, shifted due to selection. These models presume existence environmental variation can spur incipient changes in lifestyle. However, for obligate intracellular bacteria whose genomes highly reduced, studies specify discrete associations evolutionarily stable hundreds millions years. Wolbachia...
It has long been proposed that the nominal species Anastrepha fraterculus is a complex and earlier studies showed high levels of pre-zygotic isolation between two laboratory strains from Argentina Peru. Further experiments were carried out on same populations their reciprocal hybrids, including pre- post-zygotic studies, pheromone analysis, mitotic polytene chromosome analysis. A level had maintained parental despite 3 years rearing under identical conditions. The was reduced in matings with...
Ants and termites have independently evolved obligate fungus-farming mutualisms, but their gardening procedures are fundamentally different, as the predigest plant substrate whereas ants deposit it directly on fungus garden. Fungus-growing retained diverse gut microbiota, bacterial communities in fungus-growing leaf-cutting not been investigated, so is unknown whether how they specialized an exclusively fungal diet. Here we characterized community of Panamanian Acromyrmex species, which...
RNA interference (RNAi) has been widely and successfully used for gene inactivation in insects, including aphids, where dsRNA administration can be performed either by feeding or microinjection. However, several aspects related to the aphid response RNAi, as well influence of method on tissue response, mixed success observe phenotypes specific targeted, are still unclear this insect group. In present study, we made first direct comparison two methods (injection feeding) delivery targeting...
The social Hymenoptera have distinct larval and adult stages separated by metamorphosis, which implies striking remodeling of external internal body structures during the pupal stage. This imposes challenges to gut symbionts as existing cultures are lost may or not need be replaced. To elucidate extent metamorphosis interrupts associations between bacteria hosts, we analyzed changes in microbiota development traced transmission routes dominant from egg stage leaf-cutting ants Acromyrmex...
Introduction Calves are highly susceptible to gastrointestinal infection with Cryptosporidium parvum ( C. ), which can result in watery diarrhea and eventually death or impaired development. With little no effective therapeutics, understanding the host’s microbiota pathogen interaction at mucosal immune system has been critical identify test novel control strategies. Methods Herein, we used an experimental model of challenge neonatal calves describe clinical signs histological proteomic...
Abstract The stability of mutualistic interactions is likely to be affected by the genetic diversity symbionts that compete for same functional niche. Fungus‐growing (attine) ants have multiple complex symbioses and thus provide ample opportunities address questions symbiont specificity diversity. Among partners are A ctinobacteria genus P seudonocardia maintained on ant cuticle produce antibiotics, primarily against a fungal parasite gardens. symbiosis has been assumed hallmark evolutionary...
While strict vertical transmission insures the durability of intracellular symbioses, phylogenetic incongruences between hosts and endosymbionts suggest horizontal must also occur. These acquisitions can have important implications for biology host. Wolbachia is one most ecologically successful prokaryotes in arthropods, infecting an estimated 50-70% all insect species. Much this success likely due to fact that, notorious manipulating host reproduction favor through female germline. However,...
Abstract Escherichia coli is a gram-negative bacterium commonly found in mammals and birds’ intestinal microbiota. It can be easily isolated has been studied for more than nine decades, making it an ideal model organism. mainly commensal but occasionally pathogenic, expressing virulence factors, antibiotic-resistance acid-resistance genes, which contribute to its ability persist colonize diverse environments. Its multi-resistance, combined with the of certain pathotypes (e.g. STEC), poses...
Abstract Stomoxys flies are highly ubiquitous blood-feeding pests on dairy farms and suspected carriers of pathogenic bacteria due to their close association with both manure reservoirs cattle hosts. While prior studies using amplicon sequencing culture dependent methodologies have been used characterize the composition microbiota, little is known about strain level genomic functional diversity -associated bacteria. In this study, we address key gap in knowledge by whole genome provide first...
In the diverse landscape of African hominids, obligate relationship between host and its microbiome narrates signals adaptation co-evolution. Sequencing 546 hominid metagenomes, including those from indigenous Hadza wild chimpanzees, identified similar bacterial richness diversity surpassing westernized populations. While hominids share core communities, they also harbor distinct, population-specific taxa tailored to specific diets, ecology lifestyles, differentiating non-indigenous humans...
Termites host a gut microbiota of diverse and essential symbionts that enable specialization on dead plant material; an abundant, but nutritionally imbalanced food source. To supplement the severe shortage dietary nitrogen (N), some termite species make use diazotrophic bacteria to fix atmospheric (N2). Fungus-growing termites (subfamily Macrotermitinae) fungal exosymbiont (genus Termitomyces) provides digestive services main source for termites. This has been thought obviate need...
Summary Social insects owe their ecological success to the division of labour between castes, but associations microbial community compositions and castes with different tasks diets have not been extensively explored. Fungus‐growing termites associate fungi degrade plant material, complemented by diverse gut communities. Here, we explore whether accompanying dietary differences fungus‐growing termite are linked bacterial structure. Using amplicon sequencing, characterize in sterile (worker...
Fungus-growing termites rely on mutualistic fungi of the genus Termitomyces and gut microbes for plant biomass degradation. Due to a certain degree symbiont complementarity, this tripartite symbiosis has evolved as complex bioreactor, enabling decomposition nearly any polymer, likely contributing success one main decomposers in Old World. In study, we evaluated which polymers are decomposed enzymes active during process two major genera fungus-growing termites. We found diversity at...
Mollicutes, a widespread class of bacteria associated with animals and plants, were recently identified as abundant abdominal endosymbionts in healthy workers attine fungus-farming leaf-cutting ants. We obtained draft genomes the two most common strains harbored by Panamanian fungus-growing Reconstructions their functional significance showed that they are independently acquired symbionts, likely to decompose excess arginine consistent farmed fungal cultivars providing this nitrogen-rich...
Abstract Background Nutritional symbioses play a central role in insects’ adaptation to specialized diets and their evolutionary success. The obligatory symbiosis between the pea aphid, Acyrthosiphon pisum, bacterium, Buchnera aphidicola, is no exception as it enables this important agricultural pest insect develop on diet exclusively based plant phloem sap. symbiotic bacteria provide host with essential amino acids lacking its but necessary for rapid embryonic growth seen parthenogenetic...
Wolbachia is an obligatory intracellular bacterium which often manipulates the reproduction of its insect and isopod hosts. In contrast, essential symbiont in filarial nematodes. Lately, has been implicated genomic imprinting host DNA through cytosine methylation. The importance methylation cell fate biology calls for depth studing putative methylation-related genes. We present a molecular phylogenetic analysis adenine methyltransferase encoded by prophage genome. Two slightly different...
Bacterial symbionts are important fitness determinants of insects. Some hosts have independently acquired taxonomically related microbes to meet similar challenges, but whether distantly that live in tight symbiosis can maintain microbial communities has not been investigated. Varying degrees nest sharing between Megalomyrmex social parasites (Solenopsidini) and their fungus-growing ant (Attini) from the genera Cyphomyrmex, Trachymyrmex Sericomyrmex allowed us address this question, as both...
Abstract The attine ants are a monophyletic lineage that switched to fungus farming ca. 55–60 MYA. They have become model for the study of complex symbioses after additional fungal and bacterial symbionts were discovered, but their abdominal endosymbiotic bacteria remain largely unknown. Here, we present comparative microbiome analysis spanning entire phylogenetic tree. We show that, across 17 representative sympatric species from eight genera sampled in Panama, microbiomes dominated by...
Social insects maximize resource acquisition and allocation through division of labor associations with microbial symbionts. Colonies divide among castes subcastes, where the plasticity caste roles decreases in clades higher social grades. Recent studies indicate that specific may also foster distinct gut microbiomes, suggesting synergies between symbiosis. The organization a colony potentially partitions evolutionary persistent partners to optimize symbioses complement labor. However,...