A5057590266- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Cardiovascular Function and Risk Factors
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Sperm and Testicular Function
- Autophagy in Disease and Therapy
- ATP Synthase and ATPases Research
- Muscle Physiology and Disorders
- Carcinogens and Genotoxicity Assessment
- IL-33, ST2, and ILC Pathways
- Insect symbiosis and bacterial influences
- Effects and risks of endocrine disrupting chemicals
- Insect and Arachnid Ecology and Behavior
- Adipokines, Inflammation, and Metabolic Diseases
- Ultrasound and Cavitation Phenomena
- Diet and metabolism studies
- Genetic and Environmental Crop Studies
- Fibroblast Growth Factor Research
- Exercise and Physiological Responses
- Nitric Oxide and Endothelin Effects
- Agricultural pest management studies
- Bacterial Genetics and Biotechnology
- Ultrasound and Hyperthermia Applications
Laboratoire CarMeN
2015-2024
Hospices Civils de Lyon
2002-2023
Inserm
2014-2023
Université Claude Bernard Lyon 1
2014-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023
Institut National des Sciences Appliquées de Lyon
2013-2021
Biologie Fonctionnelle Insectes et Interactions
2009-2011
Hôpital de l'Antiquaille
2002
RNAIII is the intracellular effector of quorum-sensing system in Staphylococcus aureus . It one largest regulatory RNAs (514 nucleotides long) that are known to control expression a large number virulence genes. Here, we show 3′ domain coordinately represses at post-transcriptional level, mRNAs encode class factors act early infection process. We demonstrate acts primarily as an antisense RNA and rapidly anneals these mRNAs, forming long duplexes. The interaction between results repression...
Mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs) are functional domains between both organelles involved in Ca2+ exchange, through the voltage-dependent anion channel (VDAC)-1/glucose-regulated protein 75 (Grp75)/inositol 1,4,5-triphosphate receptor (IP3R)-1 complex, and regulating energy metabolism. Whereas mitochondrial dysfunction, ER stress, altered homeostasis associated with insulin signaling, implication of MAM dysfunctions resistance is unknown. Here we validated...
Modifications of the interactions between endoplasmic reticulum (ER) and mitochondria, defined as mitochondria-associated membranes (MAMs), were recently shown to be involved in control hepatic insulin action glucose homeostasis, but with conflicting results. Whereas skeletal muscle is primary site insulin-mediated uptake main target for alterations insulin-resistant states, relevance MAM integrity resistance unknown. Deciphering importance MAMs on signaling could help clarify this...
Abstract Cellular senescence is induced by stresses and results in a stable proliferation arrest accompanied pro-inflammatory secretome. Senescent cells accumulate during aging, promoting various age-related pathologies limiting lifespan. The endoplasmic reticulum (ER) inositol 1,4,5-trisphosphate receptor, type 2 (ITPR2) calcium-release channel calcium fluxes from the ER to mitochondria are drivers of human cells. Here we show that Itpr2 knockout (KO) mice display improved aging such as...
Hepatic insulin resistance in obesity and type 2 diabetes was recently associated with endoplasmic reticulum (ER)-mitochondria miscommunication. These contact sites (mitochondria-associated membranes: MAMs) are highly dynamic involved many functions; however, whether MAM dysfunction plays a causal role hepatic steatosis is not clear. Thus, we aimed to determine how organelle miscommunication the onset progression of metabolic impairment.We analyzed ER-mitochondria interactions calcium...
Imeglimin is the first in a new class of oral glucose-lowering agents currently phase 2b development. Although imeglimin improves insulin sensitivity humans, molecular mechanisms are unknown. This study used model 16-week high-fat, high-sucrose diet (HFHSD) mice to characterize its antidiabetic effects. Six-week treatment significantly decreased glycemia, restored normal glucose tolerance, and improved without modifying organs, body weights, food intake. was associated with an increase...
Mitochondria-associated endoplasmic reticulum membranes (MAM) play a key role in mitochondrial dynamics and function hepatic insulin action. Whereas mitochondria are important regulators of energy metabolism, the nutritional regulation MAM liver its adaptation physiology to nutrient availability unknown. In this study, we found that fasted postprandial transition reduced number reticulum–mitochondria contact points mouse liver. Screening potential hormonal/metabolic signals revealed glucose...
Abstract The crosstalk between different organelles allows for the exchange of proteins, lipids and ions. Endoplasmic reticulum (ER) mitochondria are physically linked signal through mitochondria-associated membrane (MAM) to regulate transfer Ca 2+ from ER stores into mitochondrial matrix, thereby affecting function intracellular homeostasis. chaperone glucose-regulated protein 75 (GRP75) is a key expressed at MAM interface which regulates ER–mitochondrial transfer. Previous studies revealed...
Obesity, through low-grade inflammation, can drive insulin resistance and type 2 diabetes. While infiltration of adipose tissue (AT) with mononuclear cells (MNCs) is well established in obesity, the functional consequences these interactions are less understood. Herein, we cocultured human adipose-derived stem (ASCs) from obese individuals MNCs analyzed their reciprocal behavior. Presence ASCs 1) enhanced interleukin (IL)-17A secretion by Th17 cells, 2) inhibited γ-interferon tumor necrosis...
Type 2 diabetic cardiomyopathy features Ca2+ signaling abnormalities, notably an altered mitochondrial handling. We here aimed to study if it might be due a dysregulation of either the whole homeostasis, reticulum-mitochondrial coupling, and/or entry through uniporter. Following 16-week high-fat high-sucrose diet (HFHSD), mice developed cardiac insulin resistance, fibrosis, hypertrophy, lipid accumulation, and diastolic dysfunction when compared standard diet. Ultrastructural proteomic...
Abstract Background Nutritional symbioses play a central role in insects’ adaptation to specialized diets and their evolutionary success. The obligatory symbiosis between the pea aphid, Acyrthosiphon pisum, bacterium, Buchnera aphidicola, is no exception as it enables this important agricultural pest insect develop on diet exclusively based plant phloem sap. symbiotic bacteria provide host with essential amino acids lacking its but necessary for rapid embryonic growth seen parthenogenetic...
Skeletal muscle atrophy is a common feature of numerous chronic pathologies and correlated with patient mortality. The REDD1 protein currently recognized as negative regulator mass through inhibition the Akt/mTORC1 signaling pathway. expression notably induced following glucocorticoid secretion, which component energy stress responses. Unexpectedly, we show here that instead limits loss during energetic stresses such hypoxia fasting by reducing glycogen depletion AMPK activation. Indeed,...
Under physiological conditions, nitric oxide (NO) produced by the endothelial NO synthase (eNOS) upregulates hepatic insulin sensitivity. Recently, contact sites between endoplasmic reticulum and mitochondria named mitochondria-associated membranes (MAMs) emerged as a crucial hub for signaling in liver. As are targets of NO, we explored whether regulates sensitivity targeting MAMs. In Huh7 cells, primary rat hepatocytes mouse livers, enhancing concentration increased MAMs, whereas inhibiting...
Chronic HCV infection causes cellular stress, fibrosis and predisposes to hepatocarcinogenesis. Mitochondria play key roles in orchestrating stress responses by regulating bioenergetics, inflammation apoptosis. To better understand the role of mitochondria viral life cycle disease progression chronic hepatitis C, we studied morphological functional mitochondrial alterations induced using productively infected hepatoma cells patient livers.Biochemical imaging assays were used assess...
Our aim was to characterize the effects and underlying mechanisms of lipid-regulating agent Niaspan<sup>®</sup> on both insulin action triglyceride decrease in 20 nondiabetic, dyslipidemic men with metabolic syndrome receiving (2 g/day) or placebo for 8 weeks a randomized, cross-over study. The plasma lipid profile were characterized at beginning end each treatment period; sensitivity assessed using 2-step euglycemic hyperinsulinemic clamp VLDL-triglyceride turnover by measuring glycerol...
Exercise is important to maintain skeletal muscle mass through stimulation of protein synthesis, which a major ATP-consuming process for cells. However, cells have face high energy demand during contraction. The present study aimed investigate synthesis regulation aerobic exercise in mouse hindlimb muscles. Male C57Bl/6J mice ran at 12 m/min 45 min or the first 25 followed by progressive increase velocity up 20 last min. Animals were injected intraperitoneally with 40 nmol/g body weight...
Diabetic cardiomyopathy (DCM) is a leading complication in type 2 diabetes patients. Recently, we have shown that the reticulum-mitochondria Ca2+ uncoupling an early and reversible trigger of cardiac dysfunction diet-induced mouse model DCM. Metformin first-line antidiabetic drug with recognized cardioprotective effect myocardial infarction. Whether metformin could prevent progression DCM remains not well understood. We therefore investigated chronic 6-week treatment on coupling function our...
Focused ultrasound involving inertial cavitation has been shown to be an efficient method induce thrombolysis without any pharmacological agent. However, further investigation of the mechanisms involved and optimization process are still required. The present work aims at studying relevance a bifrequency excitation compared classical monofrequency achieve In vitro human blood clots were placed focus piezoelectric transducer. Efficiency was assessed by weighing each clot before after...
Scope Obese adipose tissue (AT) is infiltrated by inflammatory immune cells including IL‐17A‐producing‐T (Th17) cells. It has been previously demonstrated that adipose‐derived stem from obese (ob‐ASCs), but not lean AT promote Th17 Because n‐3 PUFAs are known to inhibit inflammation, it tested here whether they could ob‐ASC‐mediated IL‐17A secretion. Methods and Results The PUFA precursor, alpha‐linolenic acid (ALA), or its derivatives, eicosapentaenoic, docosahexaenoic acid, added...
Abstract Pep29, a peptide derived from the Toxoplasma GRA5 protein, is responsible for human dendritic cellsˈ migration toward CCR7 ligand. The of DCs critical function, enabling information to be carried where immunological response occurs. Parasites are known weaken host immunity by interfering with functions and thus, may source molecules immunomodulatory properties. Here, we demonstrate that soluble GRA5, specific gondii, able increase CD34-DCs CCL19. A synthetic Pep29 hydrophilic NT...
Abstract Alterations in the strength and interface area of contact sites between endoplasmic reticulum (ER) mitochondria contribute to calcium (Ca 2+ ) dysregulation neuronal cell death, have been implicated pathology several neurodegenerative diseases. Weakening this physical linkage may reduce Ca uptake into mitochondria, while fortifying these organelle promote mitochondrial overload death. Small conductance -activated K + (SK) channels regulate respiration, their activation attenuates...
Abstract Lactate accumulation and acidification in tumours are a cancer hallmark associated with the Warburg effect. Lactic acidosis correlates malignancy, benefit it offers to has been subject of numerous hypotheses. Strikingly, lactic enhances cell survival environmental glucose depletion by repressing high-rate glycolysis fermentation, promoting an oxidative metabolism involving reactivated respiration. We used real-time NMR evaluate how cytosolic lactate up 40 mM pH 6.5 individually...