A5055062810- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Adipokines, Inflammation, and Metabolic Diseases
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Organ and Tissue Transplantation Research
- CAR-T cell therapy research
- Immune Response and Inflammation
- Immunodeficiency and Autoimmune Disorders
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- IL-33, ST2, and ILC Pathways
- Psoriasis: Treatment and Pathogenesis
- Monoclonal and Polyclonal Antibodies Research
- Reconstructive Surgery and Microvascular Techniques
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Immunotherapy and Biomarkers
- Acute Myeloid Leukemia Research
- Diabetes and associated disorders
- Rheumatoid Arthritis Research and Therapies
- Autoimmune and Inflammatory Disorders Research
- Regulation of Appetite and Obesity
- Neutropenia and Cancer Infections
Laboratoire CarMeN
2013-2024
Inserm
2014-2024
Université Claude Bernard Lyon 1
2014-2024
Hospices Civils de Lyon
2015-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2023
Hôpital Lyon Sud
1998-2023
Centre Nantais de Sociologie
2020
Institut National des Sciences Appliquées de Lyon
2017
University of Hassan II Casablanca
2015
Institut thématique Immunologie, inflammation, infectiologie et microbiologie
2013
Abstract The mechanisms underlying the immunomodulatory functions of mesenchymal stem cells (MSC) on dendritic (DC) have been shown to involve soluble factors, such as IL-6 or TGF-β, cell-cell contact, both depending report referenced. In this study, we intend clarify these by examining immunosuppressive effect human adult MSC DC differentiated from CD34+ hemopoietic progenitor (HPC). inhibit interstitial differentiation monocytes and umbilical HPC. confirm that not only halt but also...
Rheumatoid arthritis (RA) is characterized by defective bone repair and excessive destruction ankylosing spondylitis (AS) increased ectopic formation with syndesmophytes. Since TNF-α IL-17A are involved in both diseases, this study investigated their effects on the osteogenic differentiation of isolated human marrow-derived mesenchymal stem cells (hMSCs).Differentiation hMSCs into osteoblasts was induced presence or absence and/or TNF-α. Matrix mineralization (MM) evaluated alizarin red...
Obesity, through low-grade inflammation, can drive insulin resistance and type 2 diabetes. While infiltration of adipose tissue (AT) with mononuclear cells (MNCs) is well established in obesity, the functional consequences these interactions are less understood. Herein, we cocultured human adipose-derived stem (ASCs) from obese individuals MNCs analyzed their reciprocal behavior. Presence ASCs 1) enhanced interleukin (IL)-17A secretion by Th17 cells, 2) inhibited γ-interferon tumor necrosis...
Abstract Background Macrophages release not only cytokines but also extracellular vesicles (EVs). which are small membrane-derived nanovesicles with virus-like properties transferring cellular material between cells. Until now, the consequences of macrophage plasticity on and composition EVs have been poorly explored. In this study, we determined impact high-glucose (HG) concentrations metabolism, characterized their derived-EV subpopulations. Finally, whether HG-treated macrophage-derived...
Peripheral blood progenitor cells (PBPC) were mobilized and harvested in 200 patients treated for non‐Hodgkin's lymphoma ( n = 148), Hodgkin's disease 22) multiple myeloma 30). The variables predicting the collection of a minimal (>2.5 × 10 6 /kg) or high (>10 CD34 + cell count analysed. Patients with haemopoietic growth factors following either standard chemotherapy 49) high‐dose cyclophosphamide, given alone 55) combined VP16 86). received only. first mobilization resulted PBPC...
Th17 cells have been implicated in rheumatoid arthritis (RA). We hypothesized that the interaction of T with bone marrow-derived mesenchymal stem (BM-MSCs) or fibroblast- like synoviocytes (FLS) might, help cell-secreted inflammatory cytokines (i.e., interleukin-17A [IL-17A], tumor necrosis factor α [TNFα], and/or interferon-γ [IFNγ]), promote cell expansion and activation.Peripheral blood mononuclear (PBMCs) from healthy donors were cocultured BM-MSCs FLS RA patients osteoarthritis (OA)...
To investigate the production of type I interferon (IFN) by myoblasts and to identify its cell source link Toll-like receptor (TLR) C-type lectin (CLR) expression function in myositis biopsy sections.Production IFNβ was assessed cultured after stimulation with TLR-3 agonist poly(I-C) or cytokines involved Th1 Th17 differentiation. Expression HLA class molecules analyzed fluorescence-activated sorting activation neutralization. In muscle samples from patients polymyositis dermatomyositis,...
The polarization of tissue-resident macrophages is influenced by a variety signals from the immune system and local tissue environment, including nutrition. Although it known that quality quantity ingested lipids have significant effect on lipid composition extracellular vesicles their fate, unknown how nutritional environment modifies release function macrophage-derived EVs. In this study, we used combination palmitate oleate (1:2, FFA) to generate lipid-associated TREM2-expressing...
A bilateral hand allotransplantation was performed in a patient six years ago. Whereas skin is known to be highly immunogenic, grafts have been well accepted up now. Therefore, here we investigated the putative presence of regulatory T cells graft.Skin biopsies were at different time points and analyzed by immunochemistry. initially expanded with interleukin (IL)-2. In latter biopsy, directly reverse-transcriptase polymerase chain reaction without any culture.When tested against donor...
The PD-L1/PD-1 immune checkpoint axis is the strongest T cell exhaustion inducer. As dysfunction occurs during obesity, we analyzed impact of obesity on expression in white adipose tissue (WAT) mice and human adipocytes. We found that PD-L1 was overexpressed WAT diet-induced obese associated with increased PD-1 visceral but not subcutaneous WAT. Human vitro cocultures adipose-tissue-derived mesenchymal stem cells (ASC) mononuclear demonstrated presence ASC harvested from (i) enhanced as...
Synoviolin is a novel E3 ubiquitin ligase that has been implicated in the pathogenesis of rheumatoid arthritis (RA). The purpose this study was to examine expression and regulation synoviolin by tumor necrosis factor alpha (TNFalpha), both vivo vitro.A total 54 RA patients 23 healthy control subjects were analyzed before, 4 hours after first infusion, at week 22 infliximab treatment. Clinical response assessed American College Rheumatology criteria for 20% improvement Disease Activity Score...
IFN-gamma has been shown to inhibit monocyte (Mo) differentiation into mature dendritic cells (DC). Because also plays a role in tolerance induction, we asked whether this could be related generation of tolerogenic DC (Tol-DC). Toward aim, cultured Mo with GM-CSF plus IL-4 the presence or absence for 6 days and induced their maturation TNF-alpha 2 additional days. We showed that deviated from toward Tol-DC. Indeed, IFN-gamma-generated 1) expressed moderate levels costimulatory molecules, but...
Minor histocompatibility (H) Ags are classically described as self-peptides derived from intracellular proteins that expressed at the cell surface by MHC class I and II molecules induce T alloresponses. We have isolated three different populations a skin biopsy of patient suffering acute graft-versus-host disease following sex-mismatched HLA-identical bone marrow transplantation. The first population was: 1) CD4(+)/CD8(+) double-positive; 2) specific for an HLA I-restricted autosomal Ag; 3)...
Recently, we have reported that adipose tissue-derived stem cells (ASC) harvested from obese donors induce a pro-inflammatory environment when co-cultured with peripheral blood mononuclear (MNC), polarization of T toward the Th17 cell lineage, increased secretion IL-1β and IL-6 cytokines, down-regulation Th1 such as IFNγ TNFα. However, whether differentiated adipocytes, like aforementioned ASC, are in subject AT remained to be investigated. Herein, isolated ASC them into for either 8 or 14...