A5078237337- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Chronic Lymphocytic Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Cytomegalovirus and herpesvirus research
- Lymphoma Diagnosis and Treatment
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Cancer Immunotherapy and Biomarkers
- Cutaneous lymphoproliferative disorders research
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Colorectal Cancer Treatments and Studies
- Herpesvirus Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Cancer Treatment and Pharmacology
- Chronic Myeloid Leukemia Treatments
- Immunodeficiency and Autoimmune Disorders
- Acute Lymphoblastic Leukemia research
- Viral-associated cancers and disorders
- Fungal Infections and Studies
Inserm
2016-2025
Université de franche-comté
2009-2025
Université Bourgogne Franche-Comté
2007-2022
Université de Bourgogne
2017-2022
Établissement Français du Sang
2010-2021
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2015-2018
Centre Hospitalier Universitaire de Besançon
2011-2017
Structure Fédérative de Recherche en Biologie et Santé de Rennes
2015
Centre d’Investigation Clinique 1436
2013
Institut de Cancérologie de l'Ouest
2013
While the thymus is known to be essential for initial production of T cells during early life, its contribution immune development remains a matter debate. In fact, cardiac surgery in newborns, completely resected enable better access heart correct congenital defects, suggesting that it may dispensable childhood and adulthood. Here, we show young adults thymectomized exhibit an altered cell compartment. Specifically, absolute CD4+ CD8+ counts were decreased, these populations showed...
The diagnosis of plasmacytoid dendritic cell leukaemia (pDCL) is based on the immunophenotypic profile: CD4(+) CD56(+) lineage(neg) CD45RA(+)/RO(neg) CD11c(neg) CD116(low) CD123(+) CD34(neg) CD36(+) HLA-DR(+). Several studies have reported pDCL cases that do not express this exact profile or expressing some lineage antigens could thus be misdiagnosed. This study aimed to validate pDCL-specific markers for by flow-cytometry quantitative reverse transcription polymerase chain reaction bone...
Deficiency in dihydropyrimidine dehydrogenase (DPD) enzyme is the main cause of severe and lethal fluoropyrimidine-related toxicity. Various approaches have been developed for DPD-deficiency screening, including DPYD genotyping phenotyping. The goal this prospective observational study was to perform exhaustive exome sequencing examine relationships between variants toxicity advanced breast cancer patients receiving capecitabine.Two-hundred forty-three were analysed (88.5% capecitabine...
Abstract Background Patients with chronic kidney disease (CKD) are more prone to develop premature age-related diseases. Data on immune senescence scarce in CKD populations, except end-stage renal and dialysis. We designed a longitudinal prospective study evaluate at different stages its influence patient outcomes. Methods Clinical biological data collections were performed 222 patients [1–2 (n = 85), 4 53) 5 84)]. Immune biomarkers measured by cytometry T cells (CD28, CD57, CD45RA, CD31,...
Summary We determined the number and functional status of CD4+CD25high regulatory T cells (Treg) in blood samples from patients with metastatic carcinoma, evaluated their sensitivity to a single intravenous infusion cyclophosphamide. Treg numbers were significantly higher 49 cancer (9·2% CD4+ cells) compared 24 healthy donors (7·1%). These expressed transcription factor forkhead box P3 (FoxP3), glucocorticoid-induced tumour necrosis receptor family-related protein (GITR) intracellular CD152,...
The role of natural killer group 2, member D receptor (NKG2D)-expressing (NK) cells in tumor immunosurveillance is now well established. Nevertheless, progression occurs despite immunosurveillance, leading to cancer persistence immunocompetent hosts. STAT3 plays a pivotal both oncogenic functions and immunosuppression. In this study, we investigated the suppressing NK cell-mediated immunosurveillance. Using colorectal cell line (HT29) that can poorly activate NK, neutralized with...
Neuropilins, initially characterized as neuronal receptors, act co-receptors for cancer related growth factors and were recently involved in several signaling pathways leading to cytoskeletal organization, angiogenesis progression. Then, we sought investigate the ability of neuropilin-2 orchestrate epithelial-mesenchymal transition colorectal cells. Using specific siRNA target expression, or gene transfer, first observed that expression endows HT29 Colo320 xenograft formation. Moreover,...
Persistent ATG-induced CD4+ T cell lymphopenia is associated with serious clinical complications. We tested the hypothesis that ATG induces accelerated immune senescence in renal transplant recipients (RTR). Immune biomarkers were analyzed at and one-year later 97 incident RTR −62 patients receiving 35 anti-CD25 mAb (α-CD25). This consisted in: (i) thymic output; (ii) bone marrow renewal of CD34+ hematopoietic progenitor cells (CD34+HPC) lymphoid (l-HPC) myeloid (m-HPC) ratio; (iii)...
Prolonged CD4 T cell lymphopenia after administration of polyclonal anti-thymocyte globulins increases the rate posttransplantation morbidity, but whether impaired immune reconstitution affects survival is unknown. We studied effect on in 302 consecutive prevalent renal transplant recipients and role thymic function outcomes 100 incident recipients. followed cohort for a mean duration 92 months. Of these patients, 81 (27%) had persistent counts <300/mm3 36 (12%) died during follow-up....
Abstract Chronic myeloid leukemia (CML) is a chronic disease resulting in cell expansion through expression of the BCR-ABL1 fusion transcript. Tyrosine kinase inhibitors (TKI) have significantly increased survival patients with CML, and deep responders may consider stopping treatment. However, more than 50% relapse restart TKI, subsequently suffering unknown toxicity. Because CML model immune system–sensitive disease, we hypothesize that chimeric antigen receptor (CAR) T cells targeting IL1...
An IL-10 variant with higher affinity for a receptor subunit remains effective at low concentrations.
Neoplasms involving plasmacytoid dendritic cells (pDC) include blastic pDC neoplasms (BPDCN) and other proliferations, where are associated with myeloid malignancies: most frequently chronic myelomonocytic leukemia (CMML) but also acute (AML), hereafter named pDC-AML. We aimed to determine the reactive or neoplastic origin of in pDC-AML, their link CD34+ blasts, monocytes conventional DC (cDC) same sample, by phenotypic molecular analyses (targeted next-generation sequencing, 70 genes)....
Abstract Oncogenesis and ontogeny of blastic plasmacytoid dendritic cell neoplasm (BPDCN) remain uncertain, between canonical cells (pDCs) AXL+ SIGLEC6+ DCs (AS-DCs). We compared 12 BPDCN to 164 acute leukemia by Affymetrix HG-U133 Plus 2.0 arrays: were closer B-cell lymphoblastic (ALL), with enrichment in pDC, signatures, vesicular transport, deubiquitination pathways, AS-DC but only some cases. Importantly, 1 T-cell ALL clustered BPDCN, compatible morphology, immunophenotype (cCD3+ sCD3−...
Background Acute myeloid leukemia (AML) remains a very difficult disease to cure due the persistence of leukemic stem cells (LSCs), which are resistant different lines chemotherapy and basis refractory/relapsed (R/R) in 80% patients with AML not receiving allogeneic transplantation. Methods In this study, we showed that interleukin-1 receptor accessory protein (IL-1RAP) is overexpressed on cell surface LSCs all subtypes confirmed it as an interesting promising target compared most common...
The use of chimeric antigen receptor (CAR)-T cells has demonstrated excellent results in B-lymphoid malignancies. Advanced Therapy Medicinal Products (ATMP) status and good manufacturing practice (GMP) CAR-T require particular conditions production performed a pharmaceutical establishment. Our team developed new medical drug candidate for acute myeloid leukemia (AML), CAR targeting interleukin-1 accessory protein (IL-1RAP) expressed by stem cells, which will need to be evaluated phase I-IIa...
Abstract Even if adoptive cell transfer (ACT) has already shown great clinical efficiency in different types of disease, such as cancer, some adverse events consistently occur, and suicide genes are an interesting system to manage these events. Our team developed a new medical drug candidate, chimeric antigen receptor (CAR) targeting interleukin-1 accessory protein (IL-1RAP), which needs be evaluated trials with clinically applicable gene system. To prevent side effects ensure the safety our...
New-onset diabetes after transplantation (NODAT) is a serious complication of transplantation. This study tested whether IL-6 production capacity may influence the development NODAT in two different groups patients. The occurrence was analyzed with respect to gene promoter polymorphism at position -174 (G-->C) and other relevant risk factors retrospectively 217 renal transplant recipients prospectively 132. A linear increase both circulating (P = 0.09) C-reactive protein (an indicator basal...