A5064939945- Chronic Myeloid Leukemia Treatments
- Developmental Biology and Gene Regulation
- Acute Lymphoblastic Leukemia research
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- Cancer-related gene regulation
- Axon Guidance and Neuronal Signaling
- Tryptophan and brain disorders
- Genomics and Chromatin Dynamics
- Cytokine Signaling Pathways and Interactions
- Wnt/β-catenin signaling in development and cancer
- Congenital heart defects research
- Renal Transplantation Outcomes and Treatments
- Gut microbiota and health
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- T-cell and Retrovirus Studies
- Chronic Lymphocytic Leukemia Research
- Clinical Nutrition and Gastroenterology
- Dialysis and Renal Disease Management
- Telomeres, Telomerase, and Senescence
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Blood properties and coagulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Inserm
2000-2021
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2015-2021
Université Bourgogne Franche-Comté
2016-2017
Centre Hospitalier Universitaire de Besançon
2016
Sorbonne Université
2001-2015
Institut de Biologie Paris-Seine
2015
Structure Fédérative de Recherche en Biologie et Santé de Rennes
2015
Université de franche-comté
2015
Centre National de la Recherche Scientifique
1997-2013
Laboratoire de Biologie du Développement
2003-2010
TEL is a gene frequently involved in specific chromosomal translocations human leukemia and sarcoma that encodes member of the ETS family transcriptional regulators. unusual among other proteins by its ability to self-associate vivo, property essential oncogenic activation TEL-derived fusion proteins. We show here sequence-specific repressor ETS-binding site-driven transcription model natural promoters. Deletion oligomerization domain or substitution homologous region monomeric ETS1 impaired...
Wnt signaling has an important role in cell-fate determination, tissue patterning and tumorigenesis. proteins signal through seven-pass transmembrane receptors of the frizzled family to activateβ-catenin-dependent transcription target genes. Using early Xenopus embryos, we show that can dimerize dimerization is correlated with activation Wnt/β-catenin pathway. Co-immunoprecipitation studies revealed receptor Xfz3 exists as a dimer when expressed it been shown activate pathway by expression...
Persistent ATG-induced CD4+ T cell lymphopenia is associated with serious clinical complications. We tested the hypothesis that ATG induces accelerated immune senescence in renal transplant recipients (RTR). Immune biomarkers were analyzed at and one-year later 97 incident RTR −62 patients receiving 35 anti-CD25 mAb (α-CD25). This consisted in: (i) thymic output; (ii) bone marrow renewal of CD34+ hematopoietic progenitor cells (CD34+HPC) lymphoid (l-HPC) myeloid (m-HPC) ratio; (iii)...
Abstract Background Patients with chronic kidney disease (CKD) are more prone to develop premature age-related diseases. Data on immune senescence scarce in CKD populations, except end-stage renal and dialysis. We designed a longitudinal prospective study evaluate at different stages its influence patient outcomes. Methods Clinical biological data collections were performed 222 patients [1–2 (n = 85), 4 53) 5 84)]. Immune biomarkers measured by cytometry T cells (CD28, CD57, CD45RA, CD31,...
Recent studies reveal that maturation of the 40S ribosomal subunit precursors in mammals includes an additional step during processing internal transcribed spacer 1 (ITS1), when compared with yeast Saccharomyces cerevisiae, even though protein content pre-40S particle appears to be same. Here, we examine by depletion siRNA treatment function human orthologs two essential pre-ribosomal factors, hEnp1/bystin and hTsr1. Like their orthologs, bystin is required for efficient cleavage ITS1...
Myristoyl-CoA:protein N-myristoyltransferase (NMT) has recently been identified as a target for antiviral and antifungal therapy.Candida albicans is dimorphic, asexual yeast that major cause of systemic fungal infections in immunosuppressed humans.Metabolic labeling studies indicate C. d b icans synthesizes one principal 20-kDa N-myristoylprotein.The single copy NMT gene (ca-NMTl) was isolated encodes 451-amino acid protein 65% identity with Saccharomyces cerevisiae NMT. NMTl able to...
Chronic inflammation in end-stage renal disease (ESRD) is partly attributed to gut bacterial translocation (GBT) due loss of intestinal epithelium integrity. Increased levels circulating lipopolysaccharide (LPS) –a surrogate marker GBT– contribute maintain a chronic inflammatory state. However, LPS can be neutralized by lipoproteins and transported the liver for elimination. While ESRD-associated GBT has been widely described, less known about its changes impact on clinical outcome after...
Gelsolin is a 90,000-mol-wt Ca2+-binding, actin-associated protein that can nucleate actin filament growth, sever filaments, and cap barbed ends. Brevin closely related 92,000-mol-wt plasma with similar properties. has been reported to be localized on filaments in stress fibers, cardiac skeletal muscle I-bands, cellular regions where are known concentrated. Previous localization studies have used sera or antibody preparations contain brevin. Using purified brevin-free IgG IgA monoclonal...
Lack of clear identification patients at high risk acute rejection hampers the ability to individualize immunosuppressive therapy. Here we studied whether thymic function may predict in antithymocyte globulin (ATG)-treated renal transplant recipients 482 prospectively during first year post-transplant which 86 experienced rejection. Only CD45RA(+)CD31(+)CD4(+) T cell (recent emigrant [RTE]) frequency (RTE%) was marginally associated with whole population. This T-cell subset accounts for 26%...
The Glypican family of heparan sulfate proteoglycans regulates Wnt signaling and convergent extension (CE) in vertebrate embryos. They are predicted to be glycosylphosphatidylinositol (GPI)-tethered membrane-bound proteins, but there is no functional evidence their regulation by the GPI synthesis complex. Down syndrome critical region protein 5 (Dscr5, also known as Pigp) a component GPI-N-acetylglucosaminyltransferase (GPI-GnT) complex, associated with specific features syndrome. Here we...
Mesoderm and embryonic axis formation in vertebrates is mediated by maternal zygotic factors that activate the expression of target genes. Transcriptional derepression plays an important role regulation different contexts; however, its involvement possible mechanism mesoderm are largely unknown. Here we demonstrate XDSCR6, a Xenopus homologue human Down syndrome critical region protein 6 (DSCR6, or RIPPLY3), regulates through polycomb group (PcG) proteins. Xdscr6 mRNA enriched endoderm early...
In Brief Background Recent studies reported that posttransplant Epstein-Barr virus (EBV) replication is frequent and indicates overimmunosuppression. We hypothesized long-term EBV may identify overimmunosuppressed patients at higher risk of cancer. Methods analyzed a prospective cohort renal transplant recipients having routine PCR surveillance. All cancers (except EBV-related neoplasia) were recorded. Results Mean follow up was 94 + 23 months. Samples (8412) available in 669 patients. Three...
Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation. Here, we appreciated translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and other quantifying total LPS as well lipid A carbon chain length. This was done in end-stage renal (ESRD) patients healthy volunteers (HV). We observed both higher concentration significant differences composition translocated based...
Whole-mount and sectioned <I>in situ</I> hybridization was used to identify genes with restricted expression pattern in the presomitic somitic mesoderm during <I>Xenopus</I> early development. Here we report dynamic of six distinct differentially expressed these regions. These include homologues <I>purine nucleoside phosphorylase, acetylcholine receptor, aspartate aminotransferase, glycine amidinotransferase</I> brain muscle isoform <I>creatine kinases</I>. <I>Purine phosphorylase</I>...
Embryonic cell fate specification and axis patterning requires integration of several signaling pathways that orchestrate region-specific gene expression. The transcription factor signal transducer activator 3 (Stat3) plays important roles during early development, but it is unclear how Stat3 activated. Here, using Xenopus as a model, we analyzed the post-translational regulation functional consequences activation in dorsoventral patterning. We show phosphorylation, lysine methylation,...