A5006542871- Neurobiology and Insect Physiology Research
- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Memory and Neural Mechanisms
- Cholinesterase and Neurodegenerative Diseases
- Circadian rhythm and melatonin
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Genetics, Aging, and Longevity in Model Organisms
- Photochromic and Fluorescence Chemistry
- Marine Bivalve and Aquaculture Studies
- Memory Processes and Influences
- Mosquito-borne diseases and control
- Behavioral and Psychological Studies
- Neuroendocrine regulation and behavior
- Attention Deficit Hyperactivity Disorder
- Computational Drug Discovery Methods
- Insect Resistance and Genetics
- Occupational Health and Safety Research
- Olfactory and Sensory Function Studies
- bioluminescence and chemiluminescence research
- Radical Photochemical Reactions
Freie Universität Berlin
2009-2018
University Medical Center Groningen
2010
University College London
2010
SummaryAdult honey bees are maintained in vitro laboratory cages for a variety of purposes. For example, researchers may wish to perform experiments on caged individually or groups study aspects parasitology, toxicology, physiology under highly controlled conditions, they cage whole frames obtain newly emerged workers known age cohorts. Regardless purpose, must manage number variables, ranging from selection subjects (e.g. bee subspecies) experimental environment temperature and relative...
Memory retrieval initiates two consolidation processes: of an extinction memory and reconsolidation the acquisition memory. The strength processes depends on both trials is correlated with its sensitivity to inhibition. We demonstrate that in honeybee ( Apis mellifera ), a consolidated appetitive olfactory leads processes, depending number trials. Spontaneous recovery from induced by many (five), but not few (one two), blocked emetine, inhibitor protein synthesis. conclude underlies...
The health of the Western honey bee is threatened by a global epidemic deformed wing virus (DWV) infections driven ectoparasitic mite Varroa destructor acting as mechanical and biological vector. Three different variants DWV, DWV-A, -B -C exist. Virulence differences between these their relation to V. are still controversially discussed. We performed laboratory experiments analyze virulence DWV directly isolated from crippled bees (DWVP0 ) or after one additional passage in pupae (DWVP1 )....
Memory formation is a continuous process composed of multiple phases that can develop independently from each other. These depend on signaling pathways initiated after the activation receptors in different brain regions. The NMDA receptor acts as sensor coincident activity between neural inputs, and, such, its during learning thought to be crucial for various forms memory. In this study, we inhibited expression NR1 subunit honeybee using RNA interference. We show disruption mushroom body...
Conditioned behavior as observed during classical conditioning in a group of identically treated animals provides insights into the physiological process learning and memory formation. However, several studies vertebrates found remarkable difference between group-average behavioral performance characteristics individual animals. Here, we analyzed large number data (1640 animals) on olfactory honeybee ( Apis mellifera ). The acquired absolute differential differed with respect to trials,...
Honeybees (Apis mellifera) are well known for their communication and orientation skills impressive learning capability 1,2 .Because the survival of a honeybee colony depends on exploitation food sources, forager bees learn memorize variable flower sites as profitability.Forager can be easily trained in natural settings where they forage at feeding site related signals such odor or color.Appetitive associative also studied under controlled conditions laboratory by conditioning proboscis...
Abstract The honeybee (Apis mellifera) is a model organism for the study of learning and memory formation its underlying mechanisms. Honeybees have rich behaviour that can be studied in field as well laboratory. In latter case, olfactory conditioning proboscis extension response (PER) has been intensively with respect to neuronal molecular mechanisms acquisition formation. Quite lot known about pathways both unconditioned conditioned stimulus, lead identified. particular, role cAMP-protein...
Protein degradation is known to affect memory formation after extinction learning. We demonstrate here that an inhibitor of protein degradation, MG132, interferes with learning in a classical appetitive conditioning paradigm. In addition, we find enhancement when the same applied initial This result supports idea MG132 targets ongoing consolidation process. Furthermore, sensitivity and depends way on number CS-US trials intertrial interval during parameters acquisition are critical for both...
Abstract The transcription factor CREB (cAMP response element binding protein) is required for the switch from short‐term to long‐term synaptic plasticity and memory. Its activity regulated by cAMP‐dependent signalling cascade, which has been shown play a crucial role in honeybee's memory formation. To elucidate of honeybee formation we analysed CREB‐homologous gene, AmCREB , expressed as several transcripts brain. Eight have identified ( 1–8) that are generated alternate splicing. One...
In classical conditioning, the temporal sequence of stimulus presentations is critical for association between conditioned (CS) and unconditioned (US). forward CS precedes US learned as a predictor US. Thus it acquires properties to elicit behavioral response, defined excitatory properties. backward CS. The might be cessation acquiring inhibitory that inhibit response. Interestingly, behavior after conditioning controlled by both CS, but underlying mechanisms determining which these opposing...
Here, we examine the role of magnitude unconditioned stimulus (US) during classical conditioning in consolidation processes after memory retrieval. We varied US durations training and test impact these variations on retrieval with one or two conditioned stimulus-only trials. found that an extinction depends duration ruled out possibility this effect is attributable to differences satiation conditioning. conclude triggered only when reaches a critical threshold. This demonstrates cannot be...
An animal's internal state is a critical parameter required for adaptation to given environment. important aspect of an the energy that adjusted needs animal by homeostasis. Glucose one essential source energy, especially brain. A shortage glucose therefore triggers complex response restore supply. This counter-regulatory deficit includes metabolic responses like mobilization from stores and behavioral increased foraging rapid intake food. In mammals, catecholamines adrenalin noradrenalin...
Abstract The cAMP‐dependent kinase (PKA) plays a crucial part in long‐term memory formation the honeybee ( Apis mellifera ). One of putative substrates PKA activity is cAMP response element binding protein (CREB), transcription factor bZIP family. We searched genome to characterize genes from CREB/CREM and families. identified two that encode regulatory subunits three catalytic PKA. Eight code for proteins, but only one gene was found encodes member phylogenetic relationship these analysed...
Hymenopteran eusociality has been proposed to be associated with the activity of transcription factor CREB (cAMP-response element binding protein). The honeybee (Apis mellifera) is a eusocial insect displaying pronounced age-dependent division labor. In brains, CREB-dependent genes are regulated in an manner, indicating that there might role for neuronal mellifera CREB, or AmCREB) bee's this study, we further explore hypothesis by asking where brain AmCREB-dependent processes take place and...
Abstract In honeybees (Apis mellifera) the proteasome inhibitor Z-Leu-Leu-Leu-CHO (MG132) enhances long-term memory (LTM) formation. Studies in vertebrates using different inhibitors of demonstrate opposite, namely an inhibition The reason for this contradiction remains unclear. MG132 is proteasome, but also blocks other proteases. Accordingly, one possible explanation might be that proteases affected by are responsible enhancement LTM We test hypothesis comparing effect and more specific...
Abstract In the honeybee cAMP‐dependent signal transduction cascade has been implicated in processes underlying learning and memory. The protein kinase (PKA) is major mediator of cAMP action. To characterize PKA system brain we cloned a homologue catalytic subunit from honeybee. deduced amino acid sequence shows 80–94% identity with subunits Drosophila melanogaster , Aplysia californica mammals. corresponding gene predominantly expressed mushroom bodies, structure that involved memory...
This study examines the role of stimulus duration in learning and memory formation honeybees ( Apis mellifera ). In classical appetitive conditioning learn association between an initially neutral, conditioned (CS) occurrence a meaningful stimulus, unconditioned (US). Thereby CS becomes predictor for US eliciting response (CR). Here we by examining with different durations. We quantify CR during acquisition, retention, extinction early long-term (eLTM), examine molecular mechanisms eLTM...