A5014991402- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Rice Cultivation and Yield Improvement
- Genetic Mapping and Diversity in Plants and Animals
- Seed Germination and Physiology
- GABA and Rice Research
- Plant Gene Expression Analysis
- Soybean genetics and cultivation
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Plant Parasitism and Resistance
- Plant Genetic and Mutation Studies
- Biofuel production and bioconversion
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Plant responses to water stress
- Neurological diseases and metabolism
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Food composition and properties
- Ubiquitin and proteasome pathways
Nanjing Agricultural University
2015-2025
Chongqing Medical University
2024
Children's Hospital of Chongqing Medical University
2024
Pentatricopeptide repeat (PPR) proteins function in post-transcriptional regulation of organellar gene expression. Although several PPR are known to chloroplast development rice (Oryza sativa), the detailed molecular functions many remain unclear. Here, we characterized a young leaf white stripe (ylws) mutant, which has defective during early seedling growth. Map-based cloning revealed that YLWS encodes novel P-type chloroplast-targeted protein with 11 motifs. Further expression analyses...
Summary Endosperm is the main storage organ in cereal grain and determines yield quality. The molecular mechanisms of heat shock proteins regulating starch biosynthesis endosperm development remain obscure. Here, we report a rice floury mutant flo24 that develops abnormal grains central starchy cells. Map‐based cloning complementation test showed FLO24 encodes protein HSP101, which localized plastids. mutated T296I dramatically lost its ability to hydrolyze ATP rescue thermotolerance defects...
ABSTRACT Grain size is a key determinant of 1,000‐grain weight, one three factors determining grain yield. However, the complete regulatory network controlling has not been fully clarified. Here, we identified rice mutant, named small and round ( srg ) that exhibits semi‐dwarf stature size. Cytological analysis showed cell length number spikelet epidermal cells mutant are reduced, indicating SRG controls by promoting elongation increasing number. encodes kinesin belonging to kinesin‐1...
For efficient plant reproduction, seed dormancy delays germination until the environment is suitable for next generation growth and development. The phytohormone abscisic acid (ABA) plays important role in induction maintenance of dormancy. Previous studies have identified that WRKY transcription factors can regulate ABA signaling pathway. Here, we an Oswrky29 mutant with enhanced a screen T-DNA insertion population. OsWRKY29 member factor family which located nuclear. genetic analyses...
Grain size, which is determined by grain length, width, and thickness, an important determinant for yield in rice. Identification characterization of new genes that are associated with size will be helpful the improvement We characterized mutant, larger 1 (lgs1), derived from rice activation-tagged T-DNA insertion lines. Histological analysis showed increased cell numbers longitudinal direction spikelet hulls was responsible mutant phenotype lgs1. Quantitative real-time PCR (qRT-PCR) further...
Grain size is a key agronomic trait that determines the yield in plants. Regulation of grain by brassinosteroids (BRs) rice has been widely reported. However, relationship between BR signaling pathway and still requires further study. Here, we isolated mutant, named small grain2 (sg2), which displayed smaller semi-dwarf phenotype. The decreased was caused repressed cell expansion spikelet hulls sg2 mutant. Using map-based cloning combined with MutMap approach, cloned SG2, encodes...
CYP78A, a cytochrome P450 subfamily that includes rice (Oryza sativa L.) BIG GRAIN2 (BG2, CYP78A13) and Arabidopsis thaliana KLUH (KLU, CYP78A5), generate an unknown mobile growth signal (referred to as CYP78A-derived signal) increases grain (seed) size. However, the mechanism by which CYP78A pathway size remains elusive. Here, we characterized small mutant, grain4 (smg4), with smaller grains than its wild type due restricted cell expansion proliferation in spikelet hulls. SMG4 encodes...
Plant height and leaf angle are important determinants of yield in rice (Oryza sativa L.). Genes involved regulating plant were identified previous studies; however, there many remaining unknown factors that affect architecture.In this study, we characterized a dwarf mutant named ds1 with small grain size decreased angle,selected from an irradiated population ssp. japonica variety Nanjing35. The also showed abnormal floral organs. plants insensitive to BL treatment expression genes related...
Grain size is an important determinant of yield potential in crops. We previously demonstrated that natural mutations the regulatory sequences qSW5/GW5 confer grain width diversity rice. However, biological function a GW5 homolog, named GW5-Like (GW5L), remains unknown. In this study, we report on GW5L knockout mutants Kitaake, japonica cultivar (cv.) considered to have weak gw5 variant allele confers shorter and wider grains. evenly expressed various tissues, its protein product localized...
Seed dormancy is a critical trait that enhances plant survival by preventing seed germination at the wrong time or under unsuitable conditions. Lack of in rice can lead to pre-harvest sprouting on mother plants leading reduced yield and quality. Although some genes have been identified, knowledge regulation limited. Here, we characterized weak mutant named 1 (wsd1) showed higher percentage than wild-type following harvest ripeness. We cloned WSD1 encoding an aminotransferase protein using...
Grain size is one of the most important traits determining crop yield. However, mechanism controlling grain remains unclear. Here, we confirmed E3 ligase activity DECREASED GRAIN SIZE 1 (DGS1) in positive regulation rice (Oryza sativa) suggested a previous study. Rice G-protein subunit gamma 2 (RGG2), which negatively regulates size, was identified as an interacting protein DGS1. Biochemical analysis that DGS1 specifically interacts with canonical Gγ subunits (rice [RGG1] and [RGG2]) rather...
Deterioration of rice (Oryza sativa L.) affects grain quality and seed viability during storage. Lipoxygenase (LOX), a key enzyme in lipid metabolism, directly the rate ageing. Here, we found that knock-out lipoxygenase gene OsLOX1 by CRISPR/Cas9 delayed loss quality. Transcriptome analysis showed storage, affected transcription multiple genes, including genes related to metabolism antioxidant pathways such as phosphatase acetaldehyde dehydrogenase, which may regulate storability. The...
Abstract Background The plant chloroplast is essential for photosynthesis and other cellular processes, but an understanding of the biological mechanisms development are incomplete. Results A new temperature-sensitive white stripe leaf 9 ( wsl9 ) rice mutant described. develops stripes during early development, becomes green after three-leaf stage under field conditions. was albinic when grown at low temperature. Gene mapping WSL9 locus, together with complementation tests indicated that...
Specification of floral organ identity is critical for morphology and inflorescence architecture. Floral in plants controlled by homeotic A/B/C/D/E-class genes. Although multiple genes regulate organogenesis, our understanding the regulatory network remains fragmentary. Here, we characterized a rice gene KAIKOUXIAO (KKX), mutation which produces an uncharacteristic open hull, abnormal seed semi-sterility. KKX encodes putative LEUNIG-like (LUGL) transcriptional regulator OsLUGL. OsLUGL...
Abstract Specification of floral organ identity is critical for morphology and inflorescence architecture. Floral in plants controlled by homeotic A/B/C/D/E-class genes. Although multiple genes regulate organogenesis our understanding the regulatory network remains fragmentary. Here, we characterized rice gene KAIKOUXIAO (KKX), mutation which produces an uncharacteristic open hull, abnormal seed, semi-sterility. KKX encodes a putative LEUNIG-like (LUGL) transcriptional co-repressor. OsLUGL...
Abstract Pre-harvest sprouting (PHS) in cereal crops, is a universal phenomenon that affects grain yield and quality (Tai et al. 2021). Breeders use different methods to strengthen seed dormancy prevent PHS including genome editing. Abscisic acid one of the phytohormones promote dormancy. ABA8ox genes encode abscisic 8’ hydroxylase (ABA8OX) which determines level ABA content by catabolizing (Vallabhaneni Wurtzel 2010). Three rice ABA8’ hydroxylases, namely OsABA8ox1 ( LOC_Os02g47470 ),...
Abstract Storage tolerance is an important agronomic character in rice ( Oryza sativa L.). Lipoxygenase (LOX) a key enzyme lipid metabolism, and its activity directly affects the rate of aging rice. Here, lipoxygenase gene OsLOX 1 was edited by CRISPR/Cas9 Ningjing 4. We found that OsLOX1 knock-out can delay seed vigor loss, metabolism quality deterioration after aging. In addition, combining transcriptome analysis, we could conclude affected transcription multiple genes during storage,...