A5012303341- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Phylogenetic Studies
- Liver physiology and pathology
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Rice Cultivation and Yield Improvement
- Plant Reproductive Biology
- Pancreatic function and diabetes
- Polysaccharides and Plant Cell Walls
- Tissue Engineering and Regenerative Medicine
- Plant responses to water stress
- 3D Printing in Biomedical Research
- DNA Repair Mechanisms
- GABA and Rice Research
- RNA and protein synthesis mechanisms
- Plant biochemistry and biosynthesis
- Light effects on plants
- Agriculture, Soil, Plant Science
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- 14-3-3 protein interactions
- melanin and skin pigmentation
Institute of Crop Sciences
2016-2024
Chinese Academy of Agricultural Sciences
2016-2024
Nanjing Agricultural University
2013-2018
Wilkes University
2016
University of North Carolina at Charlotte
2007-2009
Temple University
1987
Crop yield potential is constrained by the inherent trade-offs among traits such as between grain size and number. Brassinosteroids (BRs) promote size, yet their role in regulating number unclear. By deciphering clustered-spikelet rice germplasm, we show that activation of BR catabolic gene BRASSINOSTEROID-DEFICIENT DWARF3 ( BRD3 ) markedly increases We establish a molecular pathway which signaling inhibitor GSK3/SHAGGY-LIKE KINASE2 phosphorylates stabilizes OsMADS1 transcriptional factor,...
Maintaining stable, high yields under fluctuating environmental conditions is a long-standing goal of crop improvement but challenging due to internal trade-off mechanisms, which are poorly understood. Here, we identify ARGONAUTE2 (AGO2) as candidate target for achieving this in rice (Oryza sativa). Overexpressing AGO2 led simultaneous increase salt tolerance and grain length. These benefits were achieved via the activation BIG GRAIN3 (BG3), encoding purine permease potentially involved...
Summary Brassinosteroids (BRs) are a class of phytohormones that modulate several important agronomic traits in rice ( Oryza sativa ). GSK2 is one the critical suppressors BR signalling and targets transcription factors such as OsBZR1 DLT to regulate responses. Here, we identified OFP3 (OVATE FAMILY PROTEIN 3) an interactor both by yeast‐two‐hybrid screening demonstrated plays distinctly negative role While knockout promoted seedling growth, overexpression led strong insensitivity, which...
Abstract Brassinosteroid (BR) phytohormones play crucial roles in regulating internode elongation rice (Oryza sativa). However, the underlying mechanism remains largely unclear. The dwarf and low-tillering (dlt) mutant is a mild BR-signaling-defective mutant. Here, we identify two dlt enhancers that show more severe shortening of lower internodes compared to uppermost (IN1). Both mutants carry alleles ORYZA SATIVA HOMEOBOX 15 (OSH15), founding gene for dwarf6-type mutants, which have...
Chloroplasts and mitochondria contain their own genomes transcriptional translational systems. Establishing these genetic systems is essential for plant growth development. Here we characterized a mutant form of Val-tRNA synthetase (OsValRS2) from Oryza sativa that targeted to both chloroplasts mitochondria. A single base change in OsValRS2 caused virescent albino phenotypes seedlings white panicles at heading. We therefore named this panicle 1 (wp1). Chlorophyll autofluorescence...
Brassinosteroids (BRs) regulate various agronomic traits such as plant height, leaf angle, and grain size in rice (Oryza sativa L.); thus, BR signaling components are promising targets for molecular rational design. However, genetic materials BR-signaling genes or family members remain limited rice. Here, by genome editing using clustered regularly interspaced short palindromic repeats (CRSPR)/Cas9 tools, we generated a panel of single, double, triple, quadruple mutants within three gene...
Grain size, which is determined by grain length, width, and thickness, an important determinant for yield in rice. Identification characterization of new genes that are associated with size will be helpful the improvement We characterized mutant, larger 1 (lgs1), derived from rice activation-tagged T-DNA insertion lines. Histological analysis showed increased cell numbers longitudinal direction spikelet hulls was responsible mutant phenotype lgs1. Quantitative real-time PCR (qRT-PCR) further...
The complex roles of the steroid hormone brassinosteroids (BRs) in many different yield- and stress-related traits make it difficult to utilize hormones for crop improvement. Here, we show that SERK2 as a BR signaling component is potentially useful candidate manipulation rice. We generated multiple mutant alleles by CRISPR/Cas9 editing knockout results compact structure accompanied with increased grain size. localized on plasma membrane can interact OsBRI1, receptor, suggesting its...
Brassinosteroids (BRs) are a class of steroid hormones with great potential for use in crop improvement. De-repression is usually one the key events hormone signaling. However, how stability GSK2, central negative regulator BR signaling rice (Oryza sativa), regulated by BRs remains elusive. Here, we identify U-box ubiquitin ligase TUD1 as GSK2-interacting protein yeast two-hybrid screening. We show that able to directly interact GSK2 and ubiquitinate protein. Phenotypes tud1 mutant highly...
Brassinosteroids (BRs) are endogenous phytohormones that play important roles in regulating plant growth and development. In this study, we evaluated the effects of brassinolide (BL, one active BRs) on soybean identified hormone multiple aspects BL application promoted hypocotyl epicotyl elongation light but blocked dark. High levels castasterone accumulated light-grown plants. disrupted shoot negative gravitropism, whereas gibberellin did not. delayed leaf senescence. Transcriptome analysis...
Abstract Leaf senescence is a complex biological process and defense responses play vital role for rice development, their molecular mechanisms, however, remain elusive in rice. We herein reported mutant spotted leaf 32 (spl32 ) derived from cultivar 9311 by radiation. The spl32 plants displayed early senescence, identified disintegration of chloroplasts as cellular evidence, dramatically decreased contents chlorophyll, up-regulation superoxide dismutase enzyme activity malondialdehyde,...
Japonica/geng and indica/xian are two major rice (Oryza sativa) subspecies with multiple divergent traits, but how these traits related interact within each remains elusive. Brassinosteroids (BRs) a class of steroid phytohormones that modulate many important agronomic in rice. Here, using different physiological assays, we revealed japonica exhibits an overall lower BR sensitivity than indica. Extensive screening signaling genes led to the identification set distributed throughout primary...
Deoxycytidine monophosphate deaminase (dCMP deaminase, DCD) is crucial to the production of dTTP needed for DNA replication and damage repair. However, effect DCD deficiency its molecular mechanism are poorly understood in plants. Here, we isolated characterized a rice albinic leaf growth retardation (alr) mutant that manifested by leaves, dwarf stature necrotic lesions. Map-based cloning complementation revealed ALR encodes protein. OsDCD was expressed ubiquitously all tissues. Enzyme...
The rapid elongation of rice (Oryza sativa) coleoptile is pivotal for the plant plumule to evade hypoxia stress induced by submergence, a condition often arising from overirrigation, ponding, rainstorms, or flooding. While brassinosteroids (BRs) are recognized their diverse roles in growth and development, influence on under hypoxic conditions remains largely unexplored. In this study, we demonstrate significant requirement BRs deep water. During Glycogen Synthase Kinase3-Like Kinase2...
There are a number of applications--ranging from temporary strategies for organ failure to pharmaceutical testing--that rely on effective bioreactor designs. The significance these devices is that they provide an environment maintaining cells in way allows them perform key cellular and tissue functions. In the current study, novel cartridge-based was developed evaluated. Its unique features include its capacity cell support adaptable design space. Specifically, it able accommodate functional...
Abstract For in vitro liver replacement devices, such as packed bed bioreactors, to maintain the essential functions of liver, they must at least successfully support hepatocytes, parenchymal cell liver. In vivo, is a major consumer oxygen. Hence it unsurprising that limited transport distance oxygen (O 2 ) governs dimensions cellular space engineered devices. Because capacity directly affects device's performance, O critical issue scale up bioreactor designs. current investigation,...