A5100420598- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Pluripotent Stem Cells Research
- Genetic factors in colorectal cancer
- Poxvirus research and outbreaks
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Plant nutrient uptake and metabolism
- Identification and Quantification in Food
- Bacillus and Francisella bacterial research
- VLSI and Analog Circuit Testing
- Integrated Circuits and Semiconductor Failure Analysis
- Genomic variations and chromosomal abnormalities
- Colorectal Cancer Treatments and Studies
- Evolution and Paleontology Studies
- Innovation and Socioeconomic Development
- Quantum-Dot Cellular Automata
- Protein Kinase Regulation and GTPase Signaling
- 3D Printing in Biomedical Research
- Bacterial Genetics and Biotechnology
- vaccines and immunoinformatics approaches
- Autophagy in Disease and Therapy
- Mosquito-borne diseases and control
- Fish biology, ecology, and behavior
Dalian Institute of Chemical Physics
2021-2025
Chinese Academy of Sciences
2016-2025
Shanghai Innovative Research Center of Traditional Chinese Medicine
2025
Soochow University
2024
First Affiliated Hospital of Soochow University
2024
Shaoxing People's Hospital
2021-2024
Zhejiang University
2021-2024
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2021-2024
South China University of Technology
2024
San Francisco State University
2024
CRISPR/Cas systems are widely used to knock out genes by inducing indel mutations, which prone genetic compensation. Complex genome modifications such as knockin (KI) might bypass compensation, though difficult practice due low efficiency. Moreover, no 'two-in-one' KI strategy combining conditional knockout (CKO) with fluorescent gene-labeling or further allele-labeling has been reported. Here, we developed a dual-cassette-donor and achieved one-step efficient generation of dual-function...
Grain size is a key agronomic trait that determines the yield in plants. Regulation of grain by brassinosteroids (BRs) rice has been widely reported. However, relationship between BR signaling pathway and still requires further study. Here, we isolated mutant, named small grain2 (sg2), which displayed smaller semi-dwarf phenotype. The decreased was caused repressed cell expansion spikelet hulls sg2 mutant. Using map-based cloning combined with MutMap approach, cloned SG2, encodes...
Accurate and early diagnosis of monkeypox virus (MPXV) is crucial for controlling epidemics treating affected individuals promptly. This study aimed to assess the analytical clinical performance MolecisionTM Monkeypox Virus qPCR Assay, Biorain ddPCR MAGLUMI® Ag (chemiluminescence immunoassay, CLIA) Assay. Additionally, it compare application antigen nucleic acid assays offer insights into using commercial assay kits. Specimens from 117 patients, serial diluted cell culture supernatant,...
Glycosylation profiling of MPXV structural proteins with poly SR.
Abstract Naive human embryonic stem cells (hESCs) that resemble the pre-implantation epiblasts are fueled by a combination of aerobic glycolysis and oxidative phosphorylation, but their mitochondrial regulators poorly understood. Here we report that, proline dehydrogenase (PRODH), mitochondria-localized metabolism enzyme, is dramatically upregulated in naive hESCs compared to primed counterparts. The upregulation PRODH induced reduction c-Myc expression dependent on PD0325901, MEK inhibitor...
STK16, a serine/threonine protein kinase, is ubiquitously expressed and conserved among all eukaryotes. STK16 has been implicated to function in variety of cellular processes such as VEGF cargo secretion, but the pathways through which these effects are mediated remain be elucidated. Through screening our focused library kinase inhibitors, we discovered highly selective ATP competitive inhibitor, STK16-IN-1, exhibits potent inhibitory activity against (IC50: 0.295 μM) with excellent...
Previous investigations on diabetic rats and palmitic corrosive instigated insulin-resistant HepG2 cells have shown that polydatin exhibits hypoglycemic hypolipidemic impacts. The AMP-activated protein kinase (AMPK) pathway assumes a crucial part in glucose lipid digestion. We aimed to investigate the regulatory system of metabolism through AMPK pathway. Glucose take-up, utilization levels, oil red O recoloring were distinguished confirm their impact improving insulin resistance. A Western...
Abstract The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement 5′-NGG-3′ protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences. Here, we report that a ortholog Staphylococcus aureus (SaCas9), and KKH variant, successfully induced targeted mutagenesis with high frequency Confirming previous findings, SpCas9 VQR, can also induce mutations Bioinformatics analysis these new Cas targets...
The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a serious public health threat. Most vaccines against SARS-CoV-2 target the highly glycosylated spike protein (S). A good knowledge of glycosylation profile this key to successful vaccine development. Unlike 22 confirmed N-glycosylation sites on S, only few O-glycosylation have been reported. This difference mainly ascribed extremely low stoichiometry O-glycosylation. Herein, we designed...
Understanding the regulation of human embryonic stem cells (hESCs) pluripotency is critical to advance field developmental biology and regenerative medicine. Despite recent progress, molecular events regulating hESC pluripotency, especially transition between naive primed states, still remain unclear. Here we show that hESCs display lower levels O-linked N-acetylglucosamine (O-GlcNAcylation) than hESCs. O-GlcNAcase (OGA), key enzyme catalyzing removal O-GlcNAc from proteins, highly expressed...
Deciphering the regulatory network for human naive and primed pluripotency is of fundamental theoretical applicable significance. Here, by combining quantitative proteomics, phosphoproteomics, acetylproteomics analyses, we revealed RNA processing translation as most differentially regulated processes between embryonic stem cells (hESCs). Although glycolytic hESCs rely predominantly on eukaryotic initiation factor 4E (eIF4E)-mediated cap-dependent pathway protein translation, with reduced...
RET fusions were discovered in non-small cell lung cancer (NSCLC), and the efficacy of RET-targeted treatment these patients has been previously established. However, with required resistance to RET-TKIs have limited options. Herein, we describe a case critical advanced adenocarcinoma harboring fusion. Despite significant response Prasetinib previously, patient developed refractory malignant pleural effusion after 24 months treatment. He was treated simultaneously intrapleural plus systemic...
FastCloning, a reliable cloning technique for plasmid construction, is widely used protocol in biomedical research laboratories. Only two-step molecular manipulations are required to add gene (cDNA) of interest into the desired vector. However, parallel multiple vectors still labor-intensive operation, which requires range primers different high-throughput projects. The situation could even be worse if fragments DNA added one plasmid. Here, we describe FastCloning (HTFC) method, by adding an...
CRC is one of the leading causes cancer mortality worldwide. Chemotherapy widely used for treatment CRC, but its efficacy remains unsatisfactory, mainly due to drug resistance. Therefore, it urgent develop new strategies overcome Combination therapy that aims achieve additive or synergistic therapeutic effects an effective approach tackle development Given established roles in tumor development, progression and metastasis, IGF-1R a promising target combination against CRC. In this study, we...
Autism spectrum disorder (ASD) affects around 1% of children with no effective blood test or cure. Recent studies have suggested that these are neurological disorders a strong genetic basis and they associated the abnormal formation dendritic spines. Chromosome microarray (CMA) together high-throughput sequencing technology has been used as powerful tool to identify new candidate genes for ASD. In present study, CMA was first scan genome-wide copy number variants in proband, clinically...
HKU1 is a human beta coronavirus and infects host cells via highly glycosylated spike protein (S). The N-glycosylation of S has been reported. However, little known about its O-glycosylation, which hinders the in-depth understanding biological functions. Herein, comprehensive study O-glycosylation was carried out based on dual-functional histidine-bonded silica (HBS) materials. enrichment method for O-glycopeptides with HBS developed validated using standard proteins. application to S1...
Abstract Research experience is enriching and inspiring for undergraduate students. experiences on advanced technologies are far from reach in level studies. In this paper, we present an approach to address gap via summer research opportunities The internship planned over 10 weeks, the student interns assigned a graduate mentor faculty advisor. This paper presents details of project, educational objectives, results obtained, surveys assessing outcomes. project related Spin Transfer Torque...
Science and Technology Program of Shenzhen [CXZZ20140903101756864, JCYJ20150924110425180, JCYJ201510301707 55264]; China Postdoctoral Foundation; International Exchange Fellowship Council [20130010]
Single-cell lineage tracing based on CRISPR/Cas9 gene editing tracks both cell states and genetic lineages at a single-cell resolution. Despite accurately dissecting fate determination, current implementations of this technology are hindered by limited recording intervals considerable dropout information. Here, we introduce new method, DuTracer, which utilizes two inducible systems to record deep history resolution, minimizing target dropouts. Application DuTracer in mouse embryoid bodies...