A5100685467- Metabolomics and Mass Spectrometry Studies
- Enzyme Catalysis and Immobilization
- Pharmacogenetics and Drug Metabolism
- Traditional Chinese Medicine Analysis
- Microbial Metabolic Engineering and Bioproduction
- Antibiotics Pharmacokinetics and Efficacy
- Ginseng Biological Effects and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Bioactive natural compounds
- Renal Transplantation Outcomes and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Antibiotic Resistance in Bacteria
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Drug Transport and Resistance Mechanisms
- Antiplatelet Therapy and Cardiovascular Diseases
- MicroRNA in disease regulation
- Inflammatory mediators and NSAID effects
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Reproductive Biology and Fertility
- Pharmacological Effects and Toxicity Studies
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Immune Cell Function and Interaction
First Affiliated Hospital of Zhengzhou University
2015-2024
Zhejiang University of Technology
2015-2024
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2010-2024
Henan Institute of Science and Technology
2009-2024
Guangxi Medical University
2021-2024
University of Electronic Science and Technology of China
2020-2024
Yangpu Hospital of Tongji University
2024
Zhengzhou University
2016-2023
Taian City Central Hospital
2021-2023
Qingdao University
2023
RNA 5-methylcytosine (m5C) modification plays critical roles in the pathogenesis of various tumors. However, function and molecular mechanism m5C tumor drug resistance remain unclear.The correlation between methylation, writer NOP2/Sun methyltransferase family member 2 (NSUN2) EGFR-TKIs was determined non-small-cell lung cancer (NSCLC) cell lines patient samples. The effects NSUN2 on were investigated by gain- loss-of-function assays vitro vivo. RNA-sequencing (RNA-seq), bisulfite sequencing...
Abstract Gene therapy holds great promise as a biomedicine for treating cancer, viral infection, and recessive gene disorders, while genetic detection has been used disease diagnosis prevention. The appropriate carrier is crucial to improve the delivery efficiency of nucleic acids. Although nonviral vectors have developed, their poor acid loading efficiency, off‐target toxicity, immunogenicity significantly hamper use. Metal‐organic frameworks (MOFs) attracted lot attention owing high...
Coumarin–chalcone hybrid is an interesting template for medicinal chemists to develop therapeutic agents with diverse pharmacological activity.
Hypoxia is a hallmark of many solid tumors, and it causes the overexpression variety proteins including epidermal growth factor receptor (EGFR). Many antitumor prodrugs have been designed to target hypoxia. Here we report identification kind hypoxia-activated proteolysis targeting chimera (ha-PROTAC) by introducing leaving group (1-methyl-2-nitro-1H-imidazol-5-yl)methyl or 4-nitrobenzyl into structure an EGFRDel19-based PROTAC. Among obtained molecules, ha-PROTAC 13 exhibits more potent...
Abstract Centrosomal proteins are necessary components of the centrosome, a conserved eukaryotic organelle essential to reproductive process. However, few centrosomal have been genetically linked fertility. Herein we identify homozygous missense variant CEP128 (c.665 G > A [p.R222Q]) in two infertile males. Remarkably, male knock-in mice harboring orthologous R222Q show anomalies sperm morphology, count, and motility. Moreover, Cep128 knock-out manifest infertility associated with...
The signaling mediated by stress-activated MAP kinases (MAPK), c-Jun N-terminal kinase (JNK) has well-established importance in cancer. In the present report, we investigated effects of curcumin on pathway human gastric cancer BGC-823 cells. Curcumin induced reactive oxygen species (ROS) production and cells apoptosis. Inhibition ROS generation antioxidant (NAC or Trion) significantly prevented curcumin-mediated Notably, observed that activated ASK1, a MAPKKK is oxidative stress sensitive...
Non-small cell lung cancer (NSCLC) is the leading cause of cancer-associated mortality in United States. Chemotherapy prolongs survival rates among patients with advanced disease, however, this at cost clinically significant adverse effects. Matrine an active component traditional Chinese medicine and a promising alternative drug for treatment NSCLC. In present study, therapeutic effects underlying molecular mechanisms matrine on A549 NSCLC line were investigated. A high concentration (1.0...
Parkinson is the second common neurodegenerative disease. The characteristics of Parkinson's disease (PD) are dopamin neurons loss caused by neuroinflammation responses. C alycosin, an isoflavone phytoestrogen isolated from Astragalus membranaceus, has multiple pharmacological activities, such as anti-inflammation, anti-tumor, and neuroprotective effects. However, it unknown whether calycosin can mitigate PD symptoms. This study aims to explore alleviate symptoms underlying mechanisms. was...
MicroRNAs (miRs) are short endogenous non-coding RNAs that act as posttranscriptional regulatory factors of gene expression. Downregulation miR-1 has been reported in gastric cancer; however, the mechanisms underlying its functions via target genes cancer remain largely unknown. The purpose this study was to investigate mechanism by which inhibits cell proliferation and migration. effects on migration were determined MTT wound-healing assays. Cell protein expression MET analyzed Western...
Background: Curcumin, a phenolic compound extracted from the rhizomes of Curcuma longa, has shown cytotoxic effects against variety cancers. The aim this study was to identify potential microRNA (miRNA) mediators anticancer curcumin in ovarian cancer cells. Materials and Methods: SKOV3 cells were treated with (<TEX>$10-60{\mu}M$</TEX>) miR-9 expression, cell proliferation, apoptosis assessed. depletion on curcumin-mediated growth suppression also examined. Phosphorylation Akt forkhead box...
This article presents the generation of monoclonal antibodies (mAbs) with high specificity against 19-nortestosterone (NT) through cell fusion techniques and development a mAb-based indirect competitive ELISA (icELISA) method colloidal gold-based immuno-chromatographic assay to detect NT residues in beef pork samples. A modified carbodiimide was employed synthesize artificial antigen, BALB/c mice were used produce anti-NT mAbs. On basis checkerboard titration, an standard curve established....
Abstract Cyclin-dependent kinases (CDKs) play important roles in the development of many types cancers by binding with their paired cyclins. However, function CDK11 larger protein isomer, p110 , tumorigenesis human breast cancer remains unclear. In present study, we explored effects and molecular mechanisms proliferation growth cells determining expression tumor tissues examining phenotypic changes after knockdown. We found that was highly expressed cell lines. Tissue microarray analysis...
tert-Butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate ((3R,5S)-CDHH) is a key intermediate of atorvastatin and rosuvastatin synthesis. Carbonyl reductase RtSCR9 from Rhodosporidium toruloides exhibited excellent activity toward tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate ((S)-CHOH). For the to be improved, random mutagenesis site-saturation were performed. Three positive mutants obtained (mut-Gln95Asp, mut-Ile144Lys, mut-Phe156Gln). These 1.94-, 3.03-, 1.61-fold 1.93-, 3.15-, 1.97-fold...
Abstract Breast cancer (BRCA) is the second leading cause of cancer‐related death among female worldwide. Recent studies have revealed that LACTB was frequently repressed and functioned as a bona fide new tumor suppressor in series cancers, including BRCA. However, molecular mechanisms underlying dysregulation BRCA not been reported. In present study, we find associated with poor prognosis by tissue microarray (TMA) analysis. Moreover, confirm direct target miR‐374a, which significantly...