A5044315992- Neonatal Respiratory Health Research
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Connective tissue disorders research
- Zebrafish Biomedical Research Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- Advanced Control Systems Optimization
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
University of Wisconsin–Madison
2021-2022
Peking University
2019
CRISPR/Cas systems are widely used to knock out genes by inducing indel mutations, which prone genetic compensation. Complex genome modifications such as knockin (KI) might bypass compensation, though difficult practice due low efficiency. Moreover, no 'two-in-one' KI strategy combining conditional knockout (CKO) with fluorescent gene-labeling or further allele-labeling has been reported. Here, we developed a dual-cassette-donor and achieved one-step efficient generation of dual-function...
Hox genes encode transcription factors that are critical for embryonic skeletal patterning and organogenesis. The Hoxa5 , Hoxb5 Hoxc5 paralogs expressed in the lung mesenchyme function redundantly during development. Conditional loss-of-function of these postnatal stages leads to severe defects alveologenesis, specifically generation elastin network, animals display bronchopulmonary dysplasia (BPD) or BPD-like phenotype. Here we show surprising results mesenchyme-specific loss Hox5 at adult...
Hox genes encode transcription factors that are important for establishing the body plan. Hoxa5 is a member of mammalian Hox5 paralogous group regulates patterning and morphology cervical-thoracic region axial skeleton. also plays crucial functions in lung morphogenesis.