A5007937797- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Antioxidant Activity and Oxidative Stress
- Aluminum toxicity and tolerance in plants and animals
- biodegradable polymer synthesis and properties
- Plant Micronutrient Interactions and Effects
- Enzyme Structure and Function
- Plant nutrient uptake and metabolism
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Genomics, phytochemicals, and oxidative stress
- Microbial Metabolic Engineering and Bioproduction
- Microplastics and Plastic Pollution
- Advanced Proteomics Techniques and Applications
- Insect Resistance and Genetics
- Plant Reproductive Biology
- Polyamine Metabolism and Applications
- Genomics and Phylogenetic Studies
- Postharvest Quality and Shelf Life Management
- Bioenergy crop production and management
- Plant responses to water stress
- Algal biology and biofuel production
- Legume Nitrogen Fixing Symbiosis
Robert W. Holley Center for Agriculture & Health
2016-2025
Cornell University
2016-2025
United States Department of Agriculture
2012-2025
Sparks
2024
Huazhong Agricultural University
2024
Agricultural Research Service
2014-2024
Ithaca College
2011-2020
Tennessee State University
2009-2018
University of North Carolina at Chapel Hill
2015
University of Wisconsin–Green Bay
2012
Significance Carotenoids are indispensable to plants and humans. Despite significant achievements in carotenoid research, we still lack the fundamental knowledge of regulatory mechanisms underlying carotenogenesis plants. Phytoene synthase (PSY) ORANGE (OR) two key proteins for biosynthesis accumulation plastids. This study shows that OR family interact directly with PSY function as major regulators active protein abundance mediating biosynthesis. The findings establish posttranscriptional...
Plastid ribosomal proteins are essential components of protein synthesis machinery and have diverse roles in plant growth development. Mutations plastid lead to a range developmental phenotypes plants. However, how they regulate these processes is not fully understood, the functions some individual remain unknown. To identify genes responsible for chloroplast development, we isolated characterized mutant that exhibited pale yellow inner leaves with reduced rate Arabidopsis. The (rps5)...
Abstract Carotenoids are essential for photosynthesis and photoprotection. Plants must evolve multifaceted regulatory mechanisms to control carotenoid biosynthesis. However, the regulators conserved among plant species remain elusive. Phytoene synthase (PSY) catalyzes highly regulated step of carotenogenesis geranylgeranyl diphosphate (GGPPS) acts as a hub interact with GGPP-utilizing enzymes synthesis specific downstream isoprenoids. Here, we report function Nudix hydrolase 23 (NUDX23),...
Chromoplasts are unique plastids that accumulate massive amounts of carotenoids. To gain a general and comparative characterization chromoplast proteins, this study performed proteomic analysis chromoplasts from six carotenoid-rich crops: watermelon, tomato, carrot, orange cauliflower, red papaya, bell pepper. Stromal membrane proteins were separated by 1D gel electrophoresis analysed using nLC-MS/MS. A total 953–2262 different crop species identified. Approximately 60% the identified...
Yellow dwarf viruses in the family Luteoviridae, which are causal agents of yellow disease cereal crops, each transmitted most efficiently by different species aphids a circulative manner that requires virus to interact with multitude aphid proteins. Aphid proteins differentially expressed F2 Schizaphis graminum genotypes segregating for ability transmit Cereal virus-RPV (CYDV-RPV) were identified using two-dimensional difference gel electrophoresis (DIGE) coupled either matrix-assisted...
Selenium (Se)-enriched broccoli has health-beneficial selenium-containing compounds, but it may contain reduced amounts of chemopreventive glucosinolates. To investigate the basis by which Se treatment influences glucosinolate levels, we treated two cultivars with 25 μM Na2SeO4. We found that supplementation suppressed accumulation total glucosinolates, particularly glucoraphanin, direct precursor a potent anticancer compound, in florets and leaves. showed suppression was not associated...
Ripening is crucial for the development of fleshy fruits that release their seeds following consumption by frugivores and are important contributors to human health nutritional security. Many genetic ripening regulators have been identified, especially in model system tomato, yet more remain be discovered integrated into comprehensive regulatory models. Most tomato genes studied pericarp tissue, though recent evidence indicates locule tissue a site early ripening-gene activities. Here, we...
Chromoplasts are plant organelles with a unique ability to sequester and store massive carotenoids. have been hypothesized enable high levels of carotenoid accumulation due enhanced sequestration or substructure formation. However, the regulators that control component formation in chromoplasts remain unknown. In melon (Cucumis melo) fruit, β-carotene is governed by ORANGE (OR), key regulator for chromoplasts. By using comparative proteomic analysis variety its isogenic line low-β mutant...
Background: Tomatoes are self-pollinating plants, and successful fruit set depends on the production of functional pollen within same flower. Our previous studies have shown that ‘Black Vernissage’ tomato variety exhibits greater resilience to heat stress in terms productivity compared ‘Micro-Tom’ variety. Pollen is determined by meiotic activity during microsporogenesis development free microspores gametogenesis. This study focused identifying (HS)-induced proteomes mother cells (PMCs)...
This study aimed to identify the aluminum (Al)-induced proteomes in tomato (Solanum lycopersicum, "Micro-Tom") after long-term exposure stress factor. Plants were treated Magnavaca's solution (pH 4.5) supplemented with 7.5 μM Al(3+) ion activity over a 4 month period beginning at emergence of flower buds and ending when lower mature leaves started turn yellow. Proteomes identified using 8-plex isobaric tags for relative absolute quantification (iTRAQ) labeling strategy followed by...
The main objective of this study was to characterize the N ‐linked glycosylation profiles recombinant hemagglutinin ( HA ) proteins expressed in either insect or plant hosts, and develop a mass spectrometry based workflow that can be used quality control assess batch‐to‐batch reproducibility for glycosylation. is surface glycoprotein influenza virus plays key role viral infectivity pathogenesis. Characterization glycans from strain A/California/04/09 H 1 1) has not yet been reported. In...
This article reports salt-induced changes in leaf and root proteomes after wild tomato ( Solanum chilense ) plants were treated with 200 m NaCl. In tissues, a total of 176 protein spots showed significant P < 0.05), which 104 induced 72 suppressed. Salt-induced proteins are associated the following pathways: photosynthesis, carbohydrate metabolism, glyoxylate shunt, glycine cleavage system, branched-chain amino acid biosynthesis, folding, defense cellular protection, signal transduction,...
Single-cell-type proteomics provides the capability to revealing genomic and information at cell-level resolution. However, methodology for this type of research has not been well-developed. This paper reports developing a workflow laser capture microdissection (LCM) followed by gel-liquid chromatography-tandem mass spectrometry (GeLC-MS/MS)-based analysis identification proteomes contained in individual cell layers tomato roots. Thin-sections (~10-μm thick, 10 sections per root tip) were...
Circulative transmission of viruses in the Luteoviridae, such as cereal yellow dwarf virus (CYDV), requires a series precisely orchestrated interactions between virus, plant, and aphid proteins. Natural selection has favored these to be retained phloem facilitate acquisition by aphids. We show that treatment infected oat tissue homogenate with sodium sulfite reduces purified Transmission electron microscopy data indicated no gross change virion morphology due treatments. However, treated...
A comparative proteomics study using isobaric tags for relative and absolute quantitation (iTRAQ) was performed on a mesophytic tomato ( Solanum lycopersicum ) cultivar dehydration-resistant wild species chilense to identify proteins that play key roles in tolerance water deficit stress. In ‘Walter’ LA3465, 130 were identified, of which 104 (80%) repressed 26 (20%) induced. S. LA1958, total 170 identified with 106 (62%) 64 (38%) According their putative molecular functions, the...
Staple grains with low levels of provitamin A carotenoids contribute to the global prevalence vitamin deficiency and therefore are main targets for biofortification. However, carotenoid stability during both seed maturation postharvest storage is a serious concern full benefits biofortified grains. In this study, we utilized Arabidopsis as model establish biofortification strategies in seeds. We discovered that manipulation biosynthetic activity by seed-specific expression Phytoene synthase...
Abstract Yellow dwarf viruses cause the most economically important virus diseases of cereal crops worldwide and are vectored by aphids. The identification vector proteins mediating transmission is critical to develop sustainable management practices understand viral strategies for circulative movement in all insect vectors. Previously, we applied 2‐D DIGE an aphid filial generation 2 population identify correlated with phenotype that were stably inherited expressed absence virus. In present...
Aluminum (Al) toxicity is a major constraint to plant growth and crop yield in acid soils. Tomato cultivars are especially susceptible excessive Al3+ accumulated the root zone. In this study, tomato plants were grown hydroponic culture system supplemented with 50 µM AlK(SO₄)₂. Seeds harvested from Al-treated contained significantly higher Al content than those control solution. these Al-enriched seeds (harvested plants) germinated AlK(SO₄)₂ solution homopiperazine-1,4-bis(2-ethanesulfonic...
Switchgrass (Panicum virgatum) is a perennial crop producing deep roots and thus highly tolerant to soil water deficit conditions. However, seedling establishment in the field very susceptible prolonged periodic drought stress. In this study, "sandwich" system simulating gradual deletion process was developed. seedlings were subjected 20-day treatment when tension increased 0.05 MPa (moderate stress) leaf physiological properties had expressed significant alteration. Drought-induced changes...
Tomato ( Solanum lycopersicum cv. Money Maker) seedlings at the two-leaf stage were grown in one-half strength Hoagland solution supplemented with 50 m NaCl for 4 days, 100 150 and a final concentration 200 2 days. Solutions refreshed every days treated untreated seedlings. Non-treated plants nonamended solution. Three biological replicates (BR) included control experiments. At end of treatments, uppermost three newly expanded leaves from all 12 each BR collected bulked to extract total...