A5091733369- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Plant Disease Resistance and Genetics
- Insect symbiosis and bacterial influences
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Resistance
- Plant-Microbe Interactions and Immunity
- Transgenic Plants and Applications
- Plant Parasitism and Resistance
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Animal Virus Infections Studies
- Plant Disease Management Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Mosquito-borne diseases and control
- Wheat and Barley Genetics and Pathology
- Phytoplasmas and Hemiptera pathogens
- Nematode management and characterization studies
- CRISPR and Genetic Engineering
- Cocoa and Sweet Potato Agronomy
Cornell University
2014-2023
Agricultural Research Service
2012-2023
Plant (United States)
2011-2023
Ithaca College
2007-2021
United States Department of Agriculture
2004-2020
Robert W. Holley Center for Agriculture & Health
2010-2018
University of Idaho
2014-2016
GTx (United States)
2002
Czech Agrifood Research Center
2000
Pennsylvania State University
1995
Proteins encoded by open reading frames (ORF) 3, 4, and 5 of the barley yellow dwarf luteovirus genome are translated from a single subgenomic RNA. The structural proteins ORF 3 (coat protein) (readthrough domain) contain undefined domains that regulate movement virus through aphid vectors. biological function nonstructural 17-kDa protein 4 is unknown. A complementation method was employed to test ability virions carrying mutations within readthrough domain be transmitted aphids cause...
A general diagnostic assay for a number of distinct luteoviruses was developed using the polymerase chain reaction (PCR) and restriction enzyme analysis. Two minimally degenerate, group-specific primers were derived from previously published RNA sequences three luteoviruses. This primer pair generated specific PCR fragments about 530 bp extracts plants infected with potato leafroll virus, beet western yellows or New York barley yellow dwarf virus (BYDV) serotypes MAV, PAV, RMV, RPV SGV,...
Numerous viruses can be transmitted by their corresponding vector insects; however, the molecular mechanisms enabling virus transmission insects have been poorly understood, especially identity of components interacting with virus. Here, we used yeast two-hybrid system to study proteomic interactions a plant (Rice stripe virus, RSV, genus Tenuivirus) its insect, small brown planthopper (Laodelphax striatellus). Sixty-six proteins L. striatellus that interacted nucleocapsid protein (pc3) RSV...
ABSTRACT Genetic bottlenecks may occur in virus populations when only a few individuals are transferred horizontally from one host to another, or viral population moves systemically the infection site. during systemic movement of an RNA plant were reported previously (H. Li and M. J. Roossinck, Virol. 78:10582-10587, 2004). In this study we mechanically inoculated artificial consisting 12 restriction enzyme marker mutants Cucumber mosaic (CMV) onto young leaves squash plants used two aphid...
Abstract Background The green peach aphid, Myzus persicae (Sulzer), is a world-wide insect pest capable of infesting more than 40 plant families, including many crop species. However, despite the significant damage inflicted by M. in agricultural systems through direct feeding and its ability to transmit viruses, limited genomic information available for this Results Sequencing 16 cDNA libraries generated 26,669 expressed sequence tags (ESTs). Aphids library construction were raised on...
The duration of access periods and the availability virus in source plants are two factors that influence transmission barley yellow dwarf (BYDV) by its aphid vectors. This study was conducted to quantify relationships among acquisition period (AAP), titer infected oats, three isolates BYDV from New York vector species. Thirteen AAPs, ranging 15 min 72 hr, were examined, quantified each leaf using enzyme-linked immunosorbent assay (ELISA) (...)
The ordinary strain of Potato virus Y (PVY), PVY O , causes mild mosaic in tobacco and induces necrosis severe stunting potato cultivars carrying the Ny gene. A novel substrain was recently reported, -O5, which is spreading United States distinguished from other isolates serologically (i.e., reacting to otherwise N -specific monoclonal antibody 1F5). To characterize this new -O5 subgroup address possible reasons for its continued spread, we conducted a molecular study North American...
Poleroviruses are restricted to vascular phloem tissues from which they transmitted by their aphid vectors and not transmissible mechanically. Phloem limitation has been attributed the absence of virus proteins either facilitating movement or counteracting plant defense. The polerovirus capsid is composed two forms coat protein, major P3 protein minor P3/P5 a translational readthrough P3. required for insect transmission acts in trans facilitate long-distance tissue. Specific potato leafroll...
Yellow dwarf viruses in the family Luteoviridae, which are causal agents of yellow disease cereal crops, each transmitted most efficiently by different species aphids a circulative manner that requires virus to interact with multitude aphid proteins. Aphid proteins differentially expressed F2 Schizaphis graminum genotypes segregating for ability transmit Cereal virus-RPV (CYDV-RPV) were identified using two-dimensional difference gel electrophoresis (DIGE) coupled either matrix-assisted...
Potato virus Y (PVY) is a serious threat to potato production due effects on tuber yield and quality, in particular, induction of necrotic ringspot disease (PTNRD), typically associated with recombinant strains PVY. These have been spreading the United States for past several years, although reasons this continuing spread remained unclear. To document assess between 2011 2015, strain composition PVY isolates circulating Columbia Basin area was determined from hundreds seed lots various...
Aphid is one of the most destructive insect pests on cultivated plants in temperate regions. Their piercing-sucking mouthparts and phloem feeding behavior directly damage crops deplete plant nutrients. Potato (Solanum tuberosum L.) important food sources planet, several aphid species, e.g., Myzus persicae (Sulzer) (green peach aphid) Macrosiphum euphorbiae (Thomas) (potato (Hemiptera: Aphididae) colonize potato transmit economically viruses. Aphid-transmitted viruses have been emerging all...
Purified particles of barley yellow dwarf luteovirus (BYDV) contain a major 22-kDa protein and minor approximately 58 kDa. The capsid is encoded by open reading frame (ORF) 3. ORF 5 immediately downstream in with 3 72-kDa can be translated via readthrough suppression the termination codon. Antibodies were produced against two Escherichia coli expressed polypeptides that represent amino- carboxyl-terminal halves putative 50-kDa 5. Immunological analyses indicated 58-kDa associated purified...
Potato leafroll virus (PLRV) capsid comprises 180 coat protein (CP) subunits, with some percentage containing a readthrough domain (RTD) extension located on the particle's surface. The RTD N terminus is highly conserved in luteovirids and this study sought to identify biologically active sites within region of PLRV RTD. Fourteen three-amino-acid-deletion mutants were generated from cloned infectious cDNA delivered plants by Agrobacterium inoculations. All mutant viruses accumulated locally...
Cereal yellow dwarf virus-RPV (CYDV-RPV) is transmitted specifically by the aphids Rhopalosiphum padi and Schizaphis graminum in a circulative nonpropagative manner. The high level of vector specificity results from having functional components receptor-mediated endocytotic pathways to allow virus transverse gut salivary tissues. Studies F(2) progeny crosses nonvector genotypes S. showed that transmission efficiency heritable trait regulated multiple genes acting an additive fashion gut-...
Summary Natural mutations in translation initiation factor eIF4E confer resistance to potyviruses many plant species. Potato is a staple food crop plagued by several potyviruses, yet date no known eIF4E‐mediated genes have been identified. In this study, we demonstrate that transgenic expression of the pvr1 2 gene from pepper confers virus Y (PVY) potato. We then use information convert susceptible potato ortholog allele into de novo for PVY using site‐directed mutagenesis. plants...
Potato virus Y (PVY) strains were originally defined by interactions with different resistance genes in standard potato cultivars. Five distinct strain groups are that cause local or systemic hypersensitive responses (HRs) genetic background a corresponding N gene: PVY(O), PVY(N), PVY(C), PVY(Z), and PVY(E). The nucleotide sequences of multiple isolates PVY(O) PVY(N) differ from each other ≈8% along their genomes. Additionally, complete genome recombinant composed segments parental...