A5101475530- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Mycobacterium research and diagnosis
- Escherichia coli research studies
- Neonatal Respiratory Health Research
- Advanced Sensor and Control Systems
- Remote Sensing and Land Use
- Bacillus and Francisella bacterial research
- Higher Education and Teaching Methods
- Corrosion Behavior and Inhibition
- Enterobacteriaceae and Cronobacter Research
- Microbial infections and disease research
- Advanced Computational Techniques and Applications
- RNA modifications and cancer
- Neonatal and fetal brain pathology
- Congenital Diaphragmatic Hernia Studies
- Cancer Research and Treatments
- Geomechanics and Mining Engineering
- Environmental and Agricultural Sciences
- Retinopathy of Prematurity Studies
Guangzhou University of Chinese Medicine
2016-2025
Guangdong Provincial Hospital of Traditional Chinese Medicine
2013-2024
Zhejiang University
2023
Huzhou University
2023
Huzhou Central Hospital
2023
Sinopec (China)
2016
Network Technologies (United States)
2016
Fourth Affiliated Hospital of Harbin Medical University
2015
ShanghaiTech University
2015
Wuhan No.1 Hospital
2015
ABSTRACT The spread of the plasmid-mediated colistin resistance gene, mcr-1 , into carbapenem-resistant Enterobacteriaceae (CRE) clinical isolates poses a significant threat to global health. Here we report identification three -harboring Escherichia coli strains, collected from patients in two provinces China. Our results show that CRE strains have started different hospitals In addition, this presents first description chromosomal integration E. strain.
Sepsis is a subtype of systemic inflammatory response syndrome (SIRS), which characterized by infection. Circulating microRNAs (miRNAs), including miR-150, miR-146a and miR-223, are potential biomarkers sepsis. In this study, we demonstrated that measuring the relative expression miR-146a/U6 in plasma, using 2-ΔΔCt method, provides method for differentiating between sepsis non-sepsis-SIRS. We observed significant increase initial cohort 6 non-sepsis-SIRS patients compared to 4 (P=0.01)...
// Yu-Qun Zeng 1,* , Zhenhua Dai 2,* Fuhua Lu 1 Zhaoyu Xusheng Liu Cha Chen 3 Pinghua Qu Dingcheng Li 4 Zhengshuang Hua 5 Yanni and Chuan Zou Department of Nephrology, The Second Clinical College, Guangzhou University Chinese Medicine Guangdong Provincial Academy Medical Sciences, Guangzhou, Guangdong, P.R. China 2 Section Immunology, Laboratory Medicine, Biomedical Statistics Informatics, Mayo Clinic, Rochester, MN, United States America State Key Biocontrol, Biodiversity Dynamics...
Five bacterial strains (SYSU YG23T, SYSU 10HL1970T, 10HP82-10, 10HL1938, 10HP457) isolated from water reservoirs of cooling systems were characterized using a polyphasic taxonomic approach. The isolates Gram-stain-negative, strictly aerobic and non-motile. Growth was enhanced in the presence l-cysteine. major fatty acids (>5 %) for five C10 : 0, C16 0 3-OH, C18 3-OH 1ω9c. Ubiquinone-8 detected as respiratory quinone while polar lipid profile consisted phosphatidylethanolamine,...
Pseudomonas aeruginosa is an opportunistic and nosocomial pathogen of humans with hundreds its virulence factors regulated by quorum sensing (QS) system. Small noncoding RNAs (sRNAs) are also key regulators bacterial virulence. However, the QS regulatory sRNAs (Qrrs) that have been characterized in P. still largely unknown. Here, sRNA AmiL (PA3366.1) amiEBCRS operon PAO1 was identified as a novel Qrr transcriptome sequencing (RNA-Seq). The expression negatively las or rhl system, which RhlR...
This study investigated resistance evolution mechanisms of conjugated plasmids and bacterial hosts under different concentrations antibiotic pressure. Ancestral strain ECNX52 was constructed by introducing the
Background Long noncoding RNAs (lncRNAs) HIF1A-AS2 is upregulated in multiple human cancers and are associated with various aspects of tumor progression. However, the molecular mechanisms cervical cancer (CC) remain largely unknown. In this study, we aim to investigate expression pattern signaling pathways CC. Methods The study included a group 20 CC patients, from whom tissue specimens were collected. Additionally, three distinct cell lines (HeLa, SiHa, CaSki) utilized. Quantitative...
The effect of antibiotics on horizontal gene transfer (HGT) is controversial, and the underlying mechanism remains poorly understood. Here, using Escherichia coli SM10λπ as donor strain, which carries a chromosomally integrated RP4 plasmid, we investigated conjugational mobilizable gentamicin (Gm) resistance plasmid. results showed that an exposure to restricted survival recipient cells significantly enhanced SM10λπ-Pseudomonas aeruginosa PAO1 conjugation, was attenuated by deficiency...
Conjugation is a key mechanism for horizontal gene transfer and plays an important role in bacterial evolution, especially with respect to antibiotic resistance. However, little known about the of donor recipient cells regulation conjugation. Here, using Escherichia coli (SM10λπ)-Pseudomonas aeruginosa (PAO1) conjugation model, we demonstrated that deficiency lasI/rhlI, genes associated generation quorum sensing signals N-acyl homoserine lactones (AHLs) PAO1, or deletion AHLs receptor SdiA...
Enterobacter cloacae has recently emerged as one of the most common carbapenem-resistant Enterobacteriaceae. The emergence and spread metallo-β-lactamase-producing E. have posed an immediate threat globally. Here, we investigated molecular characteristics 84 (CREL) collected from three tertiary hospitals in China between 2012 2016. Species identification antimicrobial susceptibility testing were performed using a VITEK-2 system. Carbapenems, polymyxins B, tigecycline tested by broth...
is an opportunistic pathogen that causes acute and chronic infections in immunocompromised individuals. Small regulatory RNAs (sRNAs) regulate multiple bacterial adaptations to environmental changes, especially virulence. Our previous study showed sRNA PrrH negatively regulates the expression of a number virulence factors, such as pyocyanin, rhamnolipid, biofilm, elastase
Four strains (08HL01032 T , 09HG994, 10HP82-6 and 10HL1960) were isolated from water of air-conditioning systems various cooling towers in Guangzhou city, China. Cells Gram-stain-negative coccobacilli without flagella, catalase-positive oxidase-negative, showing no reduction nitrate, hydrolysis urea production H 2 S. Growth was characteristically enhanced the presence l -cysteine, which consistent with properties members genus Francisella . The quinone system composed ubiquinone Q-8 minor...
Clostridioides difficile, which causes life-threatening diarrheal disease, presents an urgent threat to health care systems. In this study, we present a retrospective genomic and epidemiological analysis of C. difficile in large teaching hospital. First, collected 894 nonduplicate fecal samples from patients during whole year elucidate the molecular epidemiology. We then presented detailed description population structure based on 270 isolates separated between 2015 2020 clarified genetic...
In this study, we characterized two novel species that were closely related to but hard distinguish from C . kroppenstedtii by routine identification methods used in clinical laboratories. Since all 27 C. -like isolates obtained breast specimens of female patients with mastitis, they may be potential pathogens causing mastitis. We hope perform further epidemiological investigation these strains and explore their role
Rapid and accurate detection identification of microbial pathogens causing urinary tract infections allow prompt specific treatment. We optimized specimen processing to maximize the limit (LOD) by matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) evaluated capacity combination MALDI-TOF MS urine analysis (UA) for direct bacterial from samples. The optimal volumes processed urine, formic acid/acetonitrile, supernatant spotted onto target plate were 15...
Due to the increasing multidrug resistance and limited antibiotics, polymyxin B revived as last resort for treatment of carbapenemase-producing Klebsiella pneumoniae (CRKP). Unfortunately, heteroresistance hampers monotherapy via amplification resistant subpopulation. Reliable based combinations are demanded. Ceftazidime/avibactam has been regarded a new salvage therapy against CRKP. The occurrence was confirmed by population analysis profiling (PAP). Our study demonstrated that...
The acid tolerance mechanism is important for E. coli to resist acidic conditions encountered in mammalian host intestinal tract environment. Here, we explored how the LuxR protein SdiA influences ability context of glutamate- and glutamine-dependent resistance system (AR2). First, using a growth shock assay under different stress, demonstrated that deletion sdiA SM10λπ or BW25113 lead impaired environment PH 3-6, which was restored by complementary expression SdiA. Next, transcriptome...
Objectives: Ongoing acquisition of antimicrobial resistance genes has made Morganella morganii a new clinical treatment challenge. Understanding the molecular epidemiology M. will contribute to and prevention. Methods: We undertook 6-year epidemiological investigation from three tertiary hospitals in China since 2014. Antimicrobial susceptibility testing was performed using VITEK-2 system. All isolates were screened for β-lactam plasmid-mediated quinolone by PCR. Isolates carrying...
Mature tubular epithelial cells in the adult kidney can undergo epithelial-mesenchymal transition (EMT), a phenotypic change that is linked to pathogenesis of renal interstitial fibrosis. EMT may be considered reverse mesenchymal-epithelial transition, which occurs during normal development. The Wilms’ tumor suppressor gene <i>WT1</i> and paired box 2 <i>Pax2</i> are needed induce play key roles progression nephrogenesis. However, until now, have not been tested for...
A taxonomic study was performed on strain SYSU D3-2T, isolated from coastal seawater near the estuary of Pearl River in southern China. The observed to be Gram-reaction-negative, non-motile and non-spore-forming. Cells were found coccobacilli shape. Chemotaxonomic analysis plasma membrane revealed ubiquinone-8 as respiratory quinone, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, aminophospholipid phospholipid polar...
Abstract Pseudomonas aeruginosa is an opportunistic pathogen and a leading cause of nosocomial infections. Unfortunately, P. has low antibiotic susceptibility due to several chromosomally encoded resistance genes. Hence, we carried out mechanistic studies determine how azithromycin affects quorum sensing virulence in . lasI rhlI single double mutants were constructed. We then undertook quantitative approach the optimal concentration culture time that can affect expression HSLs. Furthermore,...
As an important virulence phenotype of Escherichia coli, the regulation mechanism biofilm by non-coding RNA and quorum sensing system has not been clarified. Here, transcriptome sequencing RT-PCR analysis, we found CsrB, a carbon storage system, was positively regulated LuxR protein SdiA. Furthermore, β-galactosidase reporter assays showed that SdiA enhanced promoter transcriptional activity csrB. The consistent dynamic expression levels CsrB during coli growth were also detected. Moreover,...
In recent years, polymyxin has been used as a last-resort therapy for carbapenem-resistant bacterial infections. The emergence of heteroresistance (HR) to hampers the efficacy treatment by amplifying resistant subpopulation. However, mechanisms behind HR remain unclear. Small noncoding RNAs (sRNAs) play an important role in regulating drug resistance. purpose this study was investigate effects and sRNA on B (PB)-HR Klebsiella pneumoniae. study, novel PhaS identified transcriptome sequencing....