A5101747384- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Identification and Susceptibility Testing
- Enterobacteriaceae and Cronobacter Research
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Infections and bacterial resistance
- Antibiotic Use and Resistance
- Urinary Tract Infections Management
- Clostridium difficile and Clostridium perfringens research
- Environmental remediation with nanomaterials
- Neurological Disease Mechanisms and Treatments
- Salmonella and Campylobacter epidemiology
- Nutrition, Genetics, and Disease
- Alcohol Consumption and Health Effects
- Bee Products Chemical Analysis
- Curcumin's Biomedical Applications
- RNA and protein synthesis mechanisms
- Microscopic Colitis
- Biochemical effects in animals
- Viral Infectious Diseases and Gene Expression in Insects
- Antimicrobial Resistance in Staphylococcus
- Cancer Research and Treatments
Affiliated Hospital of Southwest Medical University
2024
Guangzhou Medical University
2013-2022
Third Affiliated Hospital of Guangzhou Medical University
2013-2022
Jining Medical University
2021
Affiliated Hospital of Xuzhou Medical College
2011
Xuzhou Medical College
2011
The global dissemination of colistin resistance encoded by mcr-1 has been attributed to extensive use in livestock, threatening efficacy medicine. emergence common pathogens, such as Escherichia coli, is particular concern. China banned the animal feed from May 1, 2017. We investigated subsequent changes prevalence animals, humans, food, and environment, genomic epidemiology mcr-1-positive E coli (MCRPEC).Sampling was done before (October December, 2016) after 2017, 2018, respectively) ban....
mcr-1-mediated colistin resistance in Enterobacteriaceae is concerning, as used treating multidrug-resistant infections. We identified trends human fecal mcr-1-positivity rates and colonization with mcr-1-positive, third-generation cephalosporin-resistant (3GC-R) Guangzhou, China, investigated the genetic contexts of mcr-1 mcr-1-positive 3GC-R strains.Fecal samples were collected from in-/out-patients submitting specimens to 3 hospitals (2011-2016). carriage assessed using iterative...
Here, we identified mcr-4.3 in Acinetobacter baumannii , which had not been previously observed to carry an mcr gene. The -harboring A. strain AB18PR065 was isolated from pig feces a slaughterhouse Guangdong Province of China.
Bloodstream infections (BSIs) caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) are potentially life-threatening and an urgent threat to public health. The present study aims clarify the characteristics of carbapenemase-encoding virulent plasmids, their interactions with host bacterium. A total 425 Kp isolates were collected from blood BSI patients nine Chinese hospitals, between 2005 2019. Integrated epidemiological genomic data showed that ST11 ST307 associated nosocomial...
Purpose: The discovery of the plasmid-mediated colistin resistance genes, mcr, revealed a mechanism transmission resistance, which is major, global public health concern especially among individuals infected with carbapenem-resistant Gram-negative bacteria. To monitor spread and epidemiology mcr convenient reliable method to detect genes in clinical isolates needed, primary care institutions. This study aimed establish restriction endonuclease-based multiplex loop-mediated isothermal...
mcr-1-mediated colistin resistance in bacteria is concerning, as used treating multidrug-resistant bacterial infections. And mcr-1-producing have been identified multiple sources. Up to 248 million people use public transportation daily China, however; hasn't studied a potential source of community-based transmission mcr-1. Herein we investigated isolates from and explored the genomic characteristics them.Surface samples were collected Guangzhou, October 2016 April 2017. Polymerase chain...
Clostridioides difficile, which causes life-threatening diarrheal disease, presents an urgent threat to health care systems. In this study, we present a retrospective genomic and epidemiological analysis of C. difficile in large teaching hospital. First, collected 894 nonduplicate fecal samples from patients during whole year elucidate the molecular epidemiology. We then presented detailed description population structure based on 270 isolates separated between 2015 2020 clarified genetic...
Purpose: The main objective of this study was to decipher the prevalence, antimicrobial resistance, major virulence genes and molecular characteristics methicillin-resistant Staphylococcus aureus (MRSA) isolated from different clinical sources in southern China. Materials methods: present performed on 187 non-duplicate S. isolates collected three tertiary hospitals Guangdong Province, China, 2010–2016. Antimicrobial susceptibility testing by disk diffusion method measuring minimum inhibitory...
Two multidrug-resistant (MDR) mcr-1-harboring Klebsiella pneumoniae isolates from patients with urinary tract infections and one MDR quasipneumoniae isolate a patient bloodstream infection were identified to carry tmexCD1-toprJ1 The addition of the efflux pump inhibitor reduced tigecycline MIC against all three by 8- 16-fold. pKQBSI104-1 was transferred K. Escherichia coli J53 via conjugation. tmexCD1-toprJ1-carrying plasmids pKP15ZE495-1 (102,569 bp) (121,996 completely sequenced analyzed.
Colistin is increasingly used as an antibiotic of last resort for treating carbapenem-resistant Enterobacteriaceae. Mobile colistin resistance gene mcr-1 has been reported in Enterobacteriaceae around the world. Of particular concern spread into carbapenemase-producing Enterobacteriaceae, which results highly drug-resistant strains that are potentially untreatable. Notably, such mcr-1-carrying isolates harboring carbapenemase genes have animals and patients with infection. Here, we report...
The emergence and transmission of the mobile colistin resistance gene (mcr-1) threatened extensive use polymyxin antimicrobials. Accumulated evidence showed that banning additive in livestock feed efficiently reduce mcr-1 prevalence, not only animals but also humans environments. However, our previous study has revealed a small proportion Escherichia coli could continually carry chromosomally-encoded mcr-1. events, indicated existence stabilized heritage potential threat antimicrobial...
In response to the spread of colistin resistance gene mcr-1, China banned use in livestock fodders. We used a time-series analysis inpatient colonization data from 2011-2019 accurately reveal associated fluctuations mcr-1 that occurred inpatients ban.
Previous studies reported the prevalence of mcr-1 among clinical infected Salmonella isolates in China. However, transmission dynamics different ecological niches were not well investigated. Our objective is to exhibit Salmonella. 598 recovered from ten hospitals; besides 936 pig faces and 167 pork samples collected January 2015 December 2017 Guangzhou, PCR sequencing used identify mcr-1-positive Antimicrobial susceptibility testing was performed with 16 antimicrobials. Conjugation, S1-PFGE,...
Abstract Background The global dissemination of colistin resistance encoded by mcr-1 has been attributed to extensive use in livestock, threatening efficacy medicine. emergence common pathogens, such as Escherichia coli , is particular concern. Therefore, China banned the animal feed from May 1 ST 2017. We investigated subsequent changes prevalence, and genomic epidemiology -positive (MCRPEC). Methods Sampling was conducted pre- (October-December 2016) post-colistin ban (October-December,...
Objective To constructe and identify the lentiviral vector carrying protein kinase B (akt1) gene markerd by Discosoma red fluorescent (DsRed). Methods Mouse cDNAs encoding 1488 kb aktl were amplified reverse transcription-polymerase chain reaction (RT-PCR). By using TA clone technology, PCR product was cloned into T vectors, BamH Ⅰ, Ecor Ⅰ double restriction enzymatic digesting parts norientatial ligated with IRES-dsred, pXZ208 T4 ligase.The correct recombination plasmids named pXZ208-akt1...