A5056429714- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Enterobacteriaceae and Cronobacter Research
- Mycobacterium research and diagnosis
- Antimicrobial Peptides and Activities
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Use and Resistance
- Pharmaceutical and Antibiotic Environmental Impacts
- Escherichia coli research studies
- Cervical Cancer and HPV Research
- Cytomegalovirus and herpesvirus research
- Antimicrobial Resistance in Staphylococcus
- Microscopic Colitis
- Infective Endocarditis Diagnosis and Management
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Bladder and Urothelial Cancer Treatments
- Genomics and Phylogenetic Studies
- Tuberculosis Research and Epidemiology
- Urinary and Genital Oncology Studies
- Gut microbiota and health
- Pneumonia and Respiratory Infections
The First Affiliated Hospital, Sun Yat-sen University
2016-2025
Sun Yat-sen University
2016-2025
Guangzhou University of Chinese Medicine
2024
State Grid Corporation of China (China)
2024
Guangdong Provincial Hospital of Traditional Chinese Medicine
2012-2021
Memorial Sloan Kettering Cancer Center
2014-2018
Shantou Central Hospital
2011
University of Kentucky
2010
Clostridium difficile is a major cause of health care-associated infection, but disagreement between diagnostic tests an ongoing barrier to clinical decision making and public reporting. Molecular are increasingly used diagnose C infection (CDI), many molecular test-positive patients lack toxins that historically defined disease, it unclear if they need treatment.To determine the natural history for treatment who toxin immunoassay negative polymerase chain reaction (PCR) positive (Tox-/PCR+)...
ABSTRACT The spread of the plasmid-mediated colistin resistance gene, mcr-1 , into carbapenem-resistant Enterobacteriaceae (CRE) clinical isolates poses a significant threat to global health. Here we report identification three -harboring Escherichia coli strains, collected from patients in two provinces China. Our results show that CRE strains have started different hospitals In addition, this presents first description chromosomal integration E. strain.
Abstract Background The recent emergence and dissemination of high-level mobile tigecycline resistance Tet(X) challenge the clinical effectiveness tigecycline, one last-resort therapeutic options for complicated infections caused by multidrug-resistant Gram-negative Gram-positive pathogens. Although tet (X) has been found in various bacterial species, less is known about phylogeographic distribution phenotypic variance different genetic variants. Methods Herein, we conducted a multiregional...
Background Vitamin D binding protein-macrophage activating factor (DBP-maf) is a potent inhibitor of tumor growth. Its activity, however, has been attributed to indirect mechanisms such as boosting the immune response by macrophages and inhibiting blood vessel growth necessary for tumors. Methods Findings In this study we show first time that DBP-maf exhibits direct effect on prostate cells in absence macrophages. demonstrated inhibitory activity proliferation studies both LNCaP PC3 cancer...
Pseudomonas aeruginosa is an opportunistic and nosocomial pathogen of humans with hundreds its virulence factors regulated by quorum sensing (QS) system. Small noncoding RNAs (sRNAs) are also key regulators bacterial virulence. However, the QS regulatory sRNAs (Qrrs) that have been characterized in P. still largely unknown. Here, sRNA AmiL (PA3366.1) amiEBCRS operon PAO1 was identified as a novel Qrr transcriptome sequencing (RNA-Seq). The expression negatively las or rhl system, which RhlR...
This study investigated resistance evolution mechanisms of conjugated plasmids and bacterial hosts under different concentrations antibiotic pressure. Ancestral strain ECNX52 was constructed by introducing the
Surveillance systems revealed that the prevalence of vancomycin-resistant
Though droplet digital PCR (ddPCR) has emerged as a promising tool for early pathogen detection in bloodstream infections (BSIs), more studies are needed to support its clinical application widely due different ddPCR platforms with discrepant diagnostic performance. Additionally, there is still lack of data reveal the association between loads detected by and corresponding BSIs. In this prospective study, 173 patients suspected BSIs were enrolled. A multiplex assay was used detect 18...
The effect of antibiotics on horizontal gene transfer (HGT) is controversial, and the underlying mechanism remains poorly understood. Here, using Escherichia coli SM10λπ as donor strain, which carries a chromosomally integrated RP4 plasmid, we investigated conjugational mobilizable gentamicin (Gm) resistance plasmid. results showed that an exposure to restricted survival recipient cells significantly enhanced SM10λπ-Pseudomonas aeruginosa PAO1 conjugation, was attenuated by deficiency...
Conjugation is a key mechanism for horizontal gene transfer and plays an important role in bacterial evolution, especially with respect to antibiotic resistance. However, little known about the of donor recipient cells regulation conjugation. Here, using Escherichia coli (SM10λπ)-Pseudomonas aeruginosa (PAO1) conjugation model, we demonstrated that deficiency lasI/rhlI, genes associated generation quorum sensing signals N-acyl homoserine lactones (AHLs) PAO1, or deletion AHLs receptor SdiA...
Enterobacter cloacae has recently emerged as one of the most common carbapenem-resistant Enterobacteriaceae. The emergence and spread metallo-β-lactamase-producing E. have posed an immediate threat globally. Here, we investigated molecular characteristics 84 (CREL) collected from three tertiary hospitals in China between 2012 2016. Species identification antimicrobial susceptibility testing were performed using a VITEK-2 system. Carbapenems, polymyxins B, tigecycline tested by broth...
Bloodstream infection (BSI) caused by carbapenem resistant Klebsiella pneumoniae (CRKP), especially in elderly patients, results higher morbidity and mortality. The purpose of this study was to assess risk factors associated with CRKP BSI short-term mortality among patients China.In retrospective cohort study, we enrolled 252 inpatients aged ≥ 65 years KP from January 2011 December 2020 China. Data regarding demographic, microbiological characteristics, clinical outcome were collected.Among...
Colistin has been considered a last-line option for the treatment of infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP). Heterogeneous resistance to colistin leads unexplained clinical failure CRKP. Our study aimed investigate extent heteroresistance among CRKP strains in China. A total 455 colistin-susceptible strains, collected from six tertiary care hospitals China, were characterized. The overall rate was 6.2%, as determined population analysis profiles (PAPs)....
We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time cellular analysis (RTCA) system (RTCA version 2) for detection C. difficile toxin (CDT) in stool. The limit CDT by RTCA 2 was 0.12 ng/ml. Among consecutively collected 401 diarrheal stool specimens, 53 (13.2%) were toxin-producing strains quantitative toxigenic culture (qTC); bacterial loads ranged from 3.00 × 10(1) to 3.69 10(6) CFU/ml. method detected 51 samples, resulting...
Rapid and accurate detection identification of microbial pathogens causing urinary tract infections allow prompt specific treatment. We optimized specimen processing to maximize the limit (LOD) by matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) evaluated capacity combination MALDI-TOF MS urine analysis (UA) for direct bacterial from samples. The optimal volumes processed urine, formic acid/acetonitrile, supernatant spotted onto target plate were 15...
Due to the increasing multidrug resistance and limited antibiotics, polymyxin B revived as last resort for treatment of carbapenemase-producing Klebsiella pneumoniae (CRKP). Unfortunately, heteroresistance hampers monotherapy via amplification resistant subpopulation. Reliable based combinations are demanded. Ceftazidime/avibactam has been regarded a new salvage therapy against CRKP. The occurrence was confirmed by population analysis profiling (PAP). Our study demonstrated that...
The acid tolerance mechanism is important for E. coli to resist acidic conditions encountered in mammalian host intestinal tract environment. Here, we explored how the LuxR protein SdiA influences ability context of glutamate- and glutamine-dependent resistance system (AR2). First, using a growth shock assay under different stress, demonstrated that deletion sdiA SM10λπ or BW25113 lead impaired environment PH 3-6, which was restored by complementary expression SdiA. Next, transcriptome...
KPC-producing Klebsiella pneumoniae (KPC-KP) is the most widely spread carbapenem-resistant Enterobacteriaceae (CRE) in China. Avibactam a novel β-lactamase inhibitor which highly active against KPC. Recently, ceftazidime-avibactam (CAZ-AVI) was approved for clinical treatment Here we conducted retrospective study to examine antimicrobial susceptibility of CAZ-AVI prior its usage China, and evaluated potential develop resistance KPC-KP. MICs were tested 361 KPC-KP isolates collected from six...
Objectives: Ongoing acquisition of antimicrobial resistance genes has made Morganella morganii a new clinical treatment challenge. Understanding the molecular epidemiology M. will contribute to and prevention. Methods: We undertook 6-year epidemiological investigation from three tertiary hospitals in China since 2014. Antimicrobial susceptibility testing was performed using VITEK-2 system. All isolates were screened for β-lactam plasmid-mediated quinolone by PCR. Isolates carrying...
Abstract Pseudomonas aeruginosa is an opportunistic pathogen and a leading cause of nosocomial infections. Unfortunately, P. has low antibiotic susceptibility due to several chromosomally encoded resistance genes. Hence, we carried out mechanistic studies determine how azithromycin affects quorum sensing virulence in . lasI rhlI single double mutants were constructed. We then undertook quantitative approach the optimal concentration culture time that can affect expression HSLs. Furthermore,...
To study the molecular epidemiology of clinical metallo-β-lactamase (MBL)-producing Enterobacteriaceae isolates in China and to evaluate antimicrobial susceptibility MBL-Enterobacteriaceae aztreonam-avibactam.Bacterial speciation was determined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. PCR used screen for common carbapenemase genes. Antimicrobial testing antibiotics aztreonam-avibactam performed standard broth microdilution method.A total 161 were...
Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter (CR-Ent) species remain unclear in China. In this study, we performed a genomic study on 92 isolates from Enterobacter-caused infections multicenter Whole genome sequencing (WGS) was used to determine the sequence non-duplicated CR-Ent strains collected multiple tertiary health centres. The precise were identified by average nucleotide identity (ANI) silico DNA–DNA hybridization (isDDH). Molecular...
In recent years, polymyxin has been used as a last-resort therapy for carbapenem-resistant bacterial infections. The emergence of heteroresistance (HR) to hampers the efficacy treatment by amplifying resistant subpopulation. However, mechanisms behind HR remain unclear. Small noncoding RNAs (sRNAs) play an important role in regulating drug resistance. purpose this study was investigate effects and sRNA on B (PB)-HR Klebsiella pneumoniae. study, novel PhaS identified transcriptome sequencing....
Small regulatory RNAs (sRNAs) act as key regulators in many bacterial signalling cascades. However, P. aeruginosa, the sRNAs involved quorum sensing (QS) regulation and their function are still largely unknown. Here, we explored how prrH locus sRNA influences aeruginosa virulence context of QS network. First, gain- loss-of-function studies showed that PrrH affects pyocyanin, elastase rhamnolipid production; biofilm formation; swimming swarming motility impaired viability human whole blood....