A5031194392- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Microbial Natural Products and Biosynthesis
- Enzyme Catalysis and Immobilization
- Infections and bacterial resistance
- Ammonia Synthesis and Nitrogen Reduction
- Plant responses to water stress
- Plasma Applications and Diagnostics
- Trace Elements in Health
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Advanced Proteomics Techniques and Applications
- Erythrocyte Function and Pathophysiology
- Genomics and Phylogenetic Studies
- Natural Compound Pharmacology Studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Drug Transport and Resistance Mechanisms
- Electrohydrodynamics and Fluid Dynamics
- Analytical Chemistry and Chromatography
Ruhr University Bochum
2013-2024
Non-equilibrium radio-frequency driven atmospheric-pressure plasma in He/0.6%O2 gas mixture has been used to study the reaction mechanism of plasma-generated oxygen atoms aqueous solutions. The effluent from source operated with standard and 18O-labeled O2 was treat water presence phenol as a chemical probe. Comparing mass spectrometry chromatography-mass data solutions treated under normal labeled provides clear evidence that O(aq) originating phase enters liquid reacts directly phenol,...
New antibiotics are urgently needed to address the mounting resistance challenge. In early drug discovery, one of bottlenecks is elucidation targets and mechanisms. To accelerate antibiotic research, we provide a proteomic approach for rapid classification compounds into those with precedented unprecedented modes action. We established response library Bacillus subtilis covering 91 comparator compounds, mathematical was developed aid data analysis. Comparison responses (CoPR) allows...
Abstract Pathogenic bacteria often encounter fluctuating reactive oxygen species (ROS) levels, particularly during host infection, necessitating robust redox-sensing mechanisms for survival. The LysR-type transcriptional regulator (LTTR) OxyR is a widely conserved bacterial thiol-based redox sensor. However, members of the Rhizobiales also encode LsrB, second LTTR with potential function. This study explores roles and LsrB in plant-pathogen Agrobacterium tumefaciens. Through single combined...
ABSTRACT Despite the essential function of lipopolysaccharides (LPS) in Gram-negative bacteria, it is largely unknown how exact amount this molecule outer membrane controlled. The first committed step LPS biosynthesis catalyzed by LpxC enzyme. In Escherichia coli , cellular concentration adjusted only protease organism, membrane-anchored metalloprotease FtsH. Turnover E. requires a length- and sequence-specific C-terminal degradation signal. proteins from Salmonella Yersinia Vibrio species...
The outer membrane is the first line of defense for Gram-negative bacteria and serves as a major barrier antibiotics other harmful substances. biosynthesis lipopolysaccharides (LPS), essential component membrane, must be tightly controlled both too much little LPS are toxic. In Escherichia coli, cellular level key enzyme LpxC, which catalyzes committed step in biosynthesis, adjusted by proteolysis carried out membrane-bound protease FtsH. Here, we demonstrate that LpxC degraded growth...
Acyldepsipeptide (ADEP) antibiotics effectively kill multidrug-resistant Gram-positive pathogens, including vancomycin-resistant enterococcus, penicillin-resistant Streptococcus pneumoniae (PRSP), and methicillin-resistant Staphylococcus aureus (MRSA). The antibacterial activity of ADEP depends on a new mechanism action, i.e., the deregulation bacterial protease ClpP that leads to self-digestion. Our data allow insights into mode action by providing molecular explanation for distinct...
Outer membrane vesicles (OMVs), released from Gram-negative bacteria, have been attributed to intra- and interspecies communication pathogenicity in diverse bacteria. OMVs carry various components including genetic material, toxins, signaling molecules, or proteins. Although the molecular mechanism(s) of cargo delivery is not fully understood, recent studies showed that transfer OMV content surrounding cells mediated by selective interactions. Here, we show phytopathogen Agrobacterium...
Abstract Rhodobacter capsulatus fixes atmospheric nitrogen (N 2 ) by a molybdenum (Mo)‐nitrogenase and Mo‐free iron (Fe)‐nitrogenase, whose production is induced or repressed Mo, respectively. At low nanomolar Mo concentrations, both isoenzymes are synthesized contribute to fixation. Here we examined the regulatory interplay of central transcriptional activators NifA AnfA proteome profiling. As expected from earlier studies, synthesis structural proteins Mo‐nitrogenase (NifHDK)...
A promising yet clinically unexploited antibiotic target in difficult-to-treat Gram-negative bacteria is LpxC, the key enzyme biosynthesis of lipopolysaccharides, which are major constituents outer membrane. Despite development dozens chemically diverse LpxC inhibitor molecules, it essentially unknown how counteract inhibition. Our study provides comprehensive insights into response against five different inhibitors. All compounds bound to purified from Escherichia coli. Treatment E. coli...
Trimethoprim is a folate biosynthesis inhibitor. Tetrahydrofolates are essential for the transfer of C1 units in several biochemical pathways including purine, thymine, methionine, and glycine biosynthesis. This study addressed effects inhibition on bacterial physiology.Two complementary proteomic approaches were employed to analyze response Bacillus subtilis trimethoprim. Acute changes protein synthesis rates monitored by radioactive pulse labeling newly synthesized proteins subsequent 2DE...
Bacterial biocatalysts play a key role in our transition to bio-based, post-petroleum economy. However, the discovery of new is currently limited by ability analyze genomic information and capacity functionally screening for desired activities. Here, we present simple workflow that combines functional metaproteomics metagenomics, which facilitates unmediated direct environmental samples. To identify entirety lipolytic soil sample contaminated with used cooking oil, detected all proteins...
The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation. Based on its unique ability of interkingdom gene transfer, serves as a crucial biotechnological tool for genetic manipulation plant cells. presence hundreds putative sRNAs in this organism suggests considerable impact riboregulation A. physiology. Here, we characterized the biological function sRNA PmaR that controls processes growth, motility, and virulence. Among genes...
Clorobiocin is a well-known, highly effective inhibitor of DNA gyrase belonging to the aminocoumarin antibiotics. To identify potentially novel derivatives this natural product, we conducted an untargeted investigation clorobiocin biosynthesis in known producer
The trace element copper serves as cofactor for many enzymes but is toxic at elevated concentrations. In bacteria, the intracellular level maintained by efflux systems including Cue system controlled transcription factor CueR. CueR, a member of MerR family, forms homodimers and binds monovalent ions with high affinity. It activates tolerance genes copA cueO via conserved DNA-distortion mechanism. mechanism how CueR-induced turned off not fully understood. Here, we report that Escherichia...
Lipolytic enzymes are produced by animals, plants and microorganisms. With their chemo-, regio-, enantio-specific characteristics, lipolytic important biocatalysts useful in several industrial applications. They widely used the processing of fats oils, detergents, food processing, paper cosmetics production. In this work, we a new functional metaproteomics approach to screen sediment samples Indian Bakreshwar hot spring for novel thermo- solvent-stable enzymes. We were able identify an...
Identification of the molecular target is a crucial step in evaluating novel antibiotics. To support identification, label-free method based on chromatographic co-elution has previously been developed. Target identification by coelution (TICC) exploits alteration elution profile target-bound drug versus free ion exchange (IEX) chromatography to identify potential proteins from fractions. The applicability TICC for antibiotic research investigated which proteins, that is, putative targets,...
Abstract A promising but yet clinically unexploited antibiotic target in difficult-to-treat Gram-negative bacteria is LpxC, the key enzyme biosynthesis of lipopolysaccharides (LPS), which are major constituents outer membrane. To gain insights into mode action five different LpxC inhibitors, we conducted a comparative phenotypic and proteomic analysis. All compounds bound to purified from Escherichia coli . Treatment E. with these changed cell shape stabilized suggesting that FtsH-mediated...