The World Health Organization has declared the outbreak of a novel coronavirus (SARS-CoV-2 or 2019-nCoV) as global pandemic. However, mechanisms behind infection are not yet fully understood, nor there any targeted treatments vaccines. In this study, we identified high-binding-affinity aptamers targeting SARS-CoV-2 RBD, using an ACE2 competition-based aptamer selection strategy and machine learning screening algorithm. Kd values optimized CoV2-RBD-1C CoV2-RBD-4C against RBD were 5.8 nM 19.9...

Tumor-derived exosomal proteins have emerged as promising biomarkers for cancer diagnosis, but the quantitation accuracy is hindered by large numbers of normal cell-derived exosomes. Herein, we developed a dual-target-specific aptamer recognition activated in situ connection system on exosome membrane combined with droplet digital PCR (ddPCR) (TRACER) tumor-derived PD-L1 (Exo-PD-L1 ). Leveraging high binding affinity aptamers, excellent selectivity dual-aptamer recognition, and sensitivity...

Recently, lysosome targeting chimeras (LYTACs) have emerged as a promising technology that expands the scope of targeted protein degradation to extracellular targets. However, preparation by conjugation antibody and trivalent N-acetylgalactosamine (tri-GalNAc) is complex time-consuming process. The large uncertainty in number position molecular weights result low internalization efficiency. To circumvent these problems, we developed first aptamer-based LYTAC (Apt-LYTAC) realize...

Transport of PEGylated silica nanoparticles (PSiNPs) with diameters 100, 50, and 25 nm across the blood-brain barrier (BBB) was evaluated using an in vitro BBB model based on mouse cerebral endothelial cells (bEnd.3) cultured transwell inserts within a chamber. In vivo animal experiments were further performed by noninvasive imaging ex optical after injection via carotid artery. Confocal fluorescence studies carried out to evaluate uptake PSiNPs brain cells. The results showed that can...

Abstract Microfluidic chips with nano‐scale structures have shown great potential, but the fabrication and cost issues restrict their application. Herein, we propose a conceptually new “DNA nanolithography in microfluidic chip” by using sub‐10 nm three‐dimensional DNA (TDNs) as frameworks pendant aptamer at top vertex (ApTDN‐Chip). The framework ensures that is highly ordered upright orientation, avoiding undesired orientation or crowding effects caused conventional interface processes....

Abstract Exosomal glycoproteins play important roles in many physiological and pathological functions. Herein, we developed a dual labeling strategy based on protein‐specific aptamer tagging metabolic glycan for visualizing glycosylation of specific proteins exosomes. The exosomal PD‐L1 (exoPD‐L1) was imaged situ using intramolecular fluorescence resonance energy transfer (FRET) between fluorescent aptamers bound exoPD‐L1 tags glycans introduced via labeling. This method enables...

Due to its large enhancement effect, nanostructure-based surface-enhanced Raman scattering (SERS) technology had been widely applied for bioanalysis and cell imaging. However, most SERS nanostructures suffer from poor signal reproducibility, which hinders the application of in quantitative detection. We report an etching-assisted approach synthesize SERS-active plasmonic nanoparticles with 1 nm interior nanogap multiplex detection cancer dyes methoxy poly(ethylene glycol) thiol (mPEG-SH)...

There is considerable demand for sensitive, selective, and portable detection of disease-associated proteins, particularly in clinical practice diagnostic applications. Portable devices are highly desired disease biomarkers daily life due to the advantages being simple, rapid, user-friendly, low-cost. Herein we report an enzyme-encapsulated liposome-linked immunosorbent assay sensitive proteins using personal glucose meters (PGM) quantitative readout. Liposomes encapsulating a large amount...

Exosomal programmed cell death ligand 1 (exoPD-L1) has emerged as a promising biomarker for cancer diagnosis and immunotherapy outcome prediction. However, the existing quantitation methods are incapable of addressing heterogeneity exoPD-L1 glycosylation, which been demonstrated to be institutional basis PD-L1/PD-1 interaction crucial participant in inhibiting activity CD8+ T cells. Herein, an aptamer- lectin-induced proximity ligation assay combined with quantitative real-time polymerase...

Paper-based assays such as lateral flow are good candidates for portable diagnostics owing to their user-friendly format and low cost.

Traditional immunochromatographic test strips based on colloidal gold are effective devices for portable and low-cost point-of-care (POC) testing. Nevertheless, they still suffer from the limitation of qualitative or semiquantitative tests via naked-eye detection. Replacement with other signal entities, such as magnetic particles fluorescent particles, requires professional instrumentation to obtain quantitative results. A pressure-based assay platinum nanoparticles (PtNPs) can provide...

Abstract Accurate isolation of targeted extracellular vesicle (EV) is challenging due to the antigenic heterogeneity EV subpopulations which are from different cell origins. Most lack a single marker whose expression cleanly distinguishes them mixed populations closely related EVs. Here, modular platform capable taking multiple binding events as input, performing logic computations, and producing two independent outputs for tandem microchips subpopulation isolation, developed. Taking...

Accurate tongue image segmentation is helpful to acquire correct automatic diagnosis result. However, traditional methods cannot bring satisfying results in most cases. This paper proposes an end-to-end trainable method using deep convolutional neural network based on ResNet. The proposed method, named DeepTongue, segments by a forward without preprocessing. has no restrictions of the illumination and size images. Experimental show that DeepTongue improves accuracy noticeable margin. In...

As a well-studied biochemical reduction process in environmental microbiology, extracellular electron transfer (EET) was recently discovered bacteria closely related to human health, and orthologues of flavin-based EET gene were found the genomes many species across Firmicutes, major phylum mammalian gut microbiota. However, has not yet been confirmed occur gut, presence which may have broad physiological influences. Toward this end, here we first occurrence mouse microbiotas cultured vitro....

Molecular imaging, including quantification and molecular interaction studies, plays a crucial role in visualizing analysing events occurring within cells or organisms, thus facilitating the understanding of biological processes. Moreover, imaging offers promising applications for early disease diagnosis therapeutic evaluation. Aptamers are oligonucleotides that can recognize targets with high affinity specificity by folding themselves into various three-dimensional structures, serving as...

We combined the CRISPR-Cas13a system with CMC chemical labeling, developing an approach that enables precise identification of pseudouridine (Ψ) sites at specific loci within ribosomal RNA (rRNA), messenger (mRNA) and small nuclear RNAs (snRNA). This method, good efficiency simplicity, detects Ψ through fluorescence measurement, providing a straightforward fast validation for targeted interest.

It is demonstrated that DNA can be used to control the synthesis of silver nanoplates with different morphologies using spherical seeds. UV–vis spectroscopy, transmission electron microscopy, scanning X‐ray photoelectron and Raman spectroscopy are characterize synthesized nanoparticles. Silver nanoprisms encoded by poly C G, while flower bouquets nanodiscs A T, respectively. The length found have little effect on morphology Moreover, high surface enhanced scattering enhancement ability, good...

The CRISPR-Cas9 system enables facile and efficient genome engineering in living cells organisms. Small molecule control of would allow precise temporal applications the technology. In this work, we developed s[combining low line]mall m[combining line]olecule-activated allosteric a[combining line]ptamer r[combining line]egulat[combining line]ing (SMART)-sgRNAs as general strategies to programmable editing. With SMART-sgRNAs, against different targets can be achieved, which should facilitate...

Isolation and genetic analysis of circulating fetal cells from billions maternal in peripheral blood are the cornerstone cell-based non-invasive prenatal testing. Inspired by hierarchically multivalent architecture for enhanced capture nature, an aptamer-based Hierarchically mUltivalent aNTibody mimic intERface (HUNTER) was designed with a tremendous avidity effect highly efficient non-destructive release cells. It engineered grafting Y-shaped DNA nanostructures to linear polymer chain,...

Abstract Controllable assembly and disassembly of recognition interface are vital for bioanalysis. Herein, a strategy dynamic manipulation trapping force by engineering reversible immunoaffinity microinterface (DynarFace) in herringbone chip (DynarFace‐Chip) liquid biopsy is proposed. The DynarFace assembled magnetically attracting immunomagnetic beads (IMBs) on substrate, with merits convenient operation assembly. allows accumulating attachment IMBs circulating rare cell (CRC) surfaces...