A5069353359- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Cell Image Analysis Techniques
- Gene Regulatory Network Analysis
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Photoreceptor and optogenetics research
- Cancer-related molecular mechanisms research
- bioluminescence and chemiluminescence research
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Human Pose and Action Recognition
- Protein Structure and Dynamics
- Fractal and DNA sequence analysis
- Protein Kinase Regulation and GTPase Signaling
- Antibiotic Resistance in Bacteria
- PI3K/AKT/mTOR signaling in cancer
- DNA and Biological Computing
- Metal-Organic Frameworks: Synthesis and Applications
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- 3D Shape Modeling and Analysis
- Epigenetics and DNA Methylation
National Science Library
2024-2025
Chinese Academy of Sciences
2011-2024
Army Medical University
2021-2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2024
Gansu Agricultural University
2024
University of Chinese Academy of Sciences
2020-2024
Chongqing Medical University
2024
Beijing National Laboratory for Molecular Sciences
2023-2024
Beijing VDJBio (China)
2024
Shenzhen Institutes of Advanced Technology
2019-2023
N 6 -methyladenosine (m A), a ubiquitous RNA modification, is installed by METTL3-METTL14 complex.The structure of the heterodimeric complex between methyltransferase domains (MTDs) METTL3 and METTL14 has been previously determined.However, MTDs alone possess no enzymatic activity.Here we present solution for zinc finger domain (ZFD) METTL3, inclusion which fulfills activity METTL3-METTL14.We show that ZFD specifically binds to an containing 5′-GGACU-3′ consensus sequence, but does not one...
Triple-negative breast cancers (TNBC) lacking estrogen, progesterone, and HER2 receptors account for 10% to 20% of cancer are indicative poor prognosis. The development effective treatment strategies therefore represents a pressing unmet clinical need. We previously identified molecularly targeted approach target aberrant epigenetics TNBC using peptide corresponding the SIN3 interaction domain (SID) MAD. SID selectively blocked binding SID-containing proteins paired α-helix (PAH2) SIN3,...
Synthetic biologists have attempted to solve real-world problems, such as those of bacterial biofilms, that are involved in the pathogenesis many clinical infections and difficult eliminate. To address this, we employed a blue light responding system integrated it into chromosomes Pseudomonas aeruginosa. With making rational adaptions improvements light-activated system, provided robust convenient means spatiotemporally control gene expression manipulate biological processes with minimal...
Cyanidin has been shown to have therapeutic potential in osteoarthritis. However, it is unclear whether cyanidin prevents the progression of intervertebral disc degeneration (IVDD).This study evaluates effects on IVDD vitro and vivo.Nucleus pulposus cells (NPCs) isolated from lumbar IVD 4-week-old male Sprague-Dawley (SD) rats were exposed 20 ng/mL IL-1β, then treated with different doses (0-120 µM) for 24 h. SD classified into three groups (n = 8) as follows: control (normal saline),...
Abstract Diagnostic testing of biological macromolecules is great significance for early warning disease and cancer. Nevertheless, restricted by limited surface area large steric hindrance, sensitive detection with interface‐based sensing method remains challenging. Here, a “biphasic replacement” electrochemical aptamer‐based (BRE‐AB) strategy which placed capture reaction the biomacromolecule in homogeneous solution phase replaced small diameter single‐stranded DNA to attach interface...
<title>Abstract</title> Bacterial second messenger networks transmit environmental information through both amplitude and frequency modulation strategies. However, the mechanisms by which cells decode frequency-encoded signals remain poorly understood. By reconstructing cAMP system in Pseudomonas aeruginosa, we demonstrate that frequency-to-amplitude signal conversion (FAC) emerges three distinct filtering modules into gene expression patterns. Our mathematical framework predicts high-pass...
Deleted in liver cancer 1 (DLC1) is a multi-modular Rho-GTPase-activating protein (RhoGAP) and tumor suppressor. Besides its RhoGAP domain, functions of other domains DLC1 remain largely unknown. By precipitation mass spectrometry, we identified eukaryotic elongation factor 1A1 (EF1A1) as novel partner for the sterile alpha motif (SAM) domain but not SAM DLC2. The solution structure revealed new monomeric fold with four parallel helices, similar to that DLC2 distinct from domains. Mutating...
As a well-studied biochemical reduction process in environmental microbiology, extracellular electron transfer (EET) was recently discovered bacteria closely related to human health, and orthologues of flavin-based EET gene were found the genomes many species across Firmicutes, major phylum mammalian gut microbiota. However, has not yet been confirmed occur gut, presence which may have broad physiological influences. Toward this end, here we first occurrence mouse microbiotas cultured vitro....
The structure of bacterial biofilms depends on environmental conditions, such as availability nutrients, during biofilm formation. In turn, variations in part reflect differences motility early Pseudomonas aeruginosa deprived nutrients remain dispersed a surface, whereas cells supplemented with additional cluster and form microcolonies. At the single-cell scale, how bacteria modify their to favour distinct life cycle outcomes remains poorly understood. High-throughput algorithms were used...
Purpose: The emerging epitranscriptomics offers insights into the physiopathological roles of various RNA modifications. methylase NOP2/Sun domain family member 2 (NSUN2) catalyzes 5-methylcytosine (m5C) modification mRNAs. However, role NSUN2 in corneal epithelial wound healing (CEWH) remains unknown. Here we describe functional mechanisms mediating CEWH. Methods: RT-qPCR, Western blot, dot and ELISA were used to determine expression overall m5C level during silencing or overexpression was...
In this paper, we address the challenging problem of 3D toonification, which involves transferring style an artistic domain onto a target face with stylized geometry and texture. Although fine-tuning pre-trained GAN on can produce reasonable performance, strategy has limitations in domain. particular, deteriorate original latent space, affects subsequent semantic editing, requires independent optimization storage for each new style, limiting flexibility efficient deployment. To overcome...
Abstract Background The liver is anatomically divided into eight segments based on the distribution of Glisson's triad. However, molecular mechanisms underlying each segment and its association with hepatocellular carcinoma (HCC) heterogeneity are not well understood. In this study, our objective to conduct a comprehensive multiomics profiling segmentation atlas in order investigate potential subtypes therapeutic approaches for HCC. Methods A high throughput liquid chromatography‐tandem mass...
Abstract Imine‐linked covalent organic frameworks (COFs) are garnering substantial interest in resistive random‐access memory, attributed to their superior crystallinity, excellent chemical and thermal stability, modifiable molecular structures. However, the development of high‐performance COF‐based memristors impeded by challenges such as low conjugation degree imine bonds poor electron delocalization ability. Herein, we report a protonation strategy modify donor‐acceptor (D‐A) type COFs....
Exponentially growing bacteria in a well-mixed planktonic culture are generally assumed to be physiologically and phenotypically uniform distinct from their genetically identical counterparts living biofilms. Using combination of high spatiotemporal microscopy bacterial tracking algorithm, this study, we showed that cells Pseudomonas aeruginosa differently attached surfaces even when they remained the exponential phase. We consistently observed fast- slow-attaching phenotypes coexist cells,...
Bacterial pathogens operate by tightly controlling the pathogenicity to facilitate invasion and survival in host. While small molecule inducers can be designed modulate perform studies of pathogen–host interaction, these approaches, due diffusion property chemicals, may have unintended, or pleiotropic effects that impose limitations on their use. By contrast, light provides superior spatial temporal resolution. Here, using optogenetics we reengineered GacS opportunistic pathogen Pseudomonas...
Long noncoding RNAs (lncRNAs) are important regulators of diverse biological functions. However, an extensive in-depth analysis their expression profile and function in mammalian eyes is still lacking. Here we describe comprehensive landscapes stage-dependent tissue-specific lncRNA the mouse eye.Affymetrix transcriptome array profiled signatures from six different ocular tissue subsets (i.e., cornea, lens, retina, RPE, choroid, sclera) newborn 8-week-old mice. Quantitative RT-PCR validated...
mTOR is an evolutionarily conserved serine-threonine kinase with a central role in cell growth, invasion, and metastasis of tumors, activated many cancers. The aims this study were to investigate the expression ESCC tissues its relationship progression measure changes sensitivity cells cisplatin after treated siRNA by WST-8 assays, TUNEL, RT-PCR, western blots vitro vivo. results showed that was higher specimens than normal esophageal closely correlated TNM stage ESCC. significantly...
NOESY neighbors: A single 3D 13C- and 15N-edited experiment can be used to assign aromatic side-chain resonance signals of 13C,15N-labeled proteins on the basis prior assignments backbone aliphatic groups. This strategy will improve precision protein structures, especially large proteins. Supporting information for this article is available WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z503558_s.pdf or from author. Please note: The publisher not responsible content functionality...