A5008607594- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Memory and Neural Mechanisms
- Plant and animal studies
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Photosynthetic Processes and Mechanisms
- interferon and immune responses
- Autophagy in Disease and Therapy
- Histone Deacetylase Inhibitors Research
- Neuroscience and Neuropharmacology Research
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- SARS-CoV-2 and COVID-19 Research
- Plant Parasitism and Resistance
- Pharmaceutical Quality and Counterfeiting
- Immunodeficiency and Autoimmune Disorders
- Reproductive Biology and Fertility
- Insect Resistance and Genetics
- Biosensors and Analytical Detection
Huazhong Agricultural University
2015-2025
Chinese Academy of Sciences
2021-2025
Anhui Medical University
2025
Changchun Institute of Optics, Fine Mechanics and Physics
2023-2025
First Affiliated Hospital of Anhui Medical University
2025
Xiamen University
2023-2024
Huazhong University of Science and Technology
2021-2024
Yangzhou University
2023
Wuhan University
2004-2023
Sichuan University
2018-2023
Abstract As a large family of RNA-binding proteins, pentatricopeptide repeat (PPR) proteins mediate multiple aspects RNA metabolism in eukaryotes. Binding to their target single-stranded RNAs (ssRNAs) modular and base-specific fashion, PPR can serve as designable modules for gene manipulation. However, the structural basis nucleotide-specific recognition by designer (dPPR) remains be elucidated. Here, we report four crystal structures dPPR complex with respective ssRNA targets. The repeats...
N 6 -methyladenosine (m A), a ubiquitous RNA modification, is installed by METTL3-METTL14 complex.The structure of the heterodimeric complex between methyltransferase domains (MTDs) METTL3 and METTL14 has been previously determined.However, MTDs alone possess no enzymatic activity.Here we present solution for zinc finger domain (ZFD) METTL3, inclusion which fulfills activity METTL3-METTL14.We show that ZFD specifically binds to an containing 5′-GGACU-3′ consensus sequence, but does not one...
Members of the pentatricopeptide repeat (PPR) protein family are sequence-specific RNA-binding proteins that play crucial roles in organelle RNA metabolism. Each PPR consists a tandem array motifs, each which aligns to one nucleotide target. The di-residues motif, referred as codes, determine specificity. Numerous codes distributed among vast number but correlation between and bases is poorly understood, hinders target prediction functional investigation proteins. To address this issue, we...
Abstract Phosphate (Pi) starvation response (PHR) transcription factors play key roles in plant Pi homeostasis maintenance. They are negatively regulated by stand-alone SPX proteins, cellular receptors for inositol pyrophosphate (PP-InsP) nutrient messengers. How PP-InsP-bound interacts with PHRs is poorly understood. Here, we report crystal structures of the rice SPX2/InsP 6 /PHR2 complex and PHR2 DNA binding (MYB) domain target at resolutions 3.1 Å 2.7 Å, respectively. In complex,...
Abstract Paraquat is one of the most widely used herbicides worldwide. In green plants, paraquat targets chloroplast by transferring electrons from photosystem I to molecular oxygen generate toxic reactive species, which efficiently induce membrane damage and cell death. A number paraquat-resistant biotypes weeds Arabidopsis (Arabidopsis thaliana) mutants have been identified. The herbicide resistance in partly attributed a reduced uptake through plasma membrane-localized transporters....
Middle East respiratory syndrome coronavirus (MERS-CoV) infection in humans is highly lethal, with a fatality rate of 35%. New prophylactic and therapeutic strategies to combat human infections are urgently needed. We isolated fully neutralizing antibody, MCA1, from survivor. The antibody recognizes the receptor-binding domain MERS-CoV S glycoprotein interferes interaction between viral cellular receptor dipeptidyl peptidase 4 (DPP4). To our knowledge, this study first report monoclonal that...
Abstract Purpose To compare the diagnostic accuracy of biparametric MRI (bpMRI) and multiparametric (mpMRI) for prostate cancer (PCa) clinically significant (csPCa) to explore application value dynamic contrast-enhanced (DCE) in imaging. Methods materials This study retrospectively enrolled 235 patients with suspected PCa our hospital from January 2016 December 2017, all lesions were histopathologically confirmed. The scored according Prostate Imaging Reporting Data System version 2 (PI-RADS...
Our previous studies have revealed that the signaling protein BCL10 plays a major role in adaptive immunity by mediating NF-κB activation LPS/TLR4 pathway. In this study, we show IRAK-1 acts as essential upstream adaptor recruits to TLR4 complex and mediates through BCL10-MALT1-TRAF6-TAK1 cascade. Following dissociation from IRAK-1, is translocated into cytosol along with TRAF6 TAK1, process bridged direct BCL10-Pellino2 interaction. RNA interference against MALT1 markedly reduced level of...
A simple, sensitive, reliable and economical method was developed for the determination of paraquat (a widely used herbicide) in four edible vegetables (cabbage, lettuce, spinach Chinese cabbage) using high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS-MS). The samples were extracted with water under sonication cleaned up by weak cation exchange solid-phase extraction. Chromatographic separation achieved on a hydrophilic interaction chromatography column (2.1 × 100 mm,...
Barrels that build barrels Cells produce specialized machinery to functional β-barrel proteins are involved in cross-membrane transport and membrane protein biogenesis eukaryotic organelles some bacteria. Wang et al . studied one of these systems is itself a β-barrel, the mitochondrial sorting assembly (SAM), complex with its client proteins, translocase outer (TOM). TOM subunits can be added at time while bound SAM. Compared full core complex, final subunit clashes SAM suggests release may...
The field synergy principle is widely used in the analyses and optimizations of convective heat transfer, but its original derivations are based on fluids with constant thermal properties. applicability for temperature-dependent properties needs clarification. A theoretical analysis problem conducted. It found that a mass flux vector specific enthalpy gradient more fundamental. Because temperature gradients parallel to gradients, velocity fields still valid. Heat transfer supercritical CO2...
Abstract Prokaryotic Argonaute proteins (pAgos) defend the host against invading nucleic acids, including plasmids and viruses. Short pAgo systems confer immunity by inducing cell death upon detecting acids. However, activation mechanism of SiAgo system, comprising a short from archaeon Sulfolobus islandicus its associated SiAga1 SiAga2, remains largely unknown. Here, we determined cryo-electron microscopy structures SiAgo–Aga1 apo complex RNA–DNA-bound at resolutions 2.7 3.0 Å,...