A5101486975- Heart Failure Treatment and Management
- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- Receptor Mechanisms and Signaling
- GDF15 and Related Biomarkers
- Cardiac Fibrosis and Remodeling
- Peptidase Inhibition and Analysis
- Adenosine and Purinergic Signaling
- Nuclear Receptors and Signaling
- Signaling Pathways in Disease
- Phosphodiesterase function and regulation
- Histone Deacetylase Inhibitors Research
- Ion Transport and Channel Regulation
- Cellular transport and secretion
- ATP Synthase and ATPases Research
- Cardiac electrophysiology and arrhythmias
- NF-κB Signaling Pathways
- Retinoids in leukemia and cellular processes
- Acute Kidney Injury Research
- RNA Interference and Gene Delivery
- Macrophage Migration Inhibitory Factor
- Membrane-based Ion Separation Techniques
- Ion channel regulation and function
- Protease and Inhibitor Mechanisms
- S100 Proteins and Annexins
Tulane University
2015-2024
Middlebury College
2022
Saint Barnabas Medical Center
2016
University of New Orleans
2005
Queen's University
1999
Augusta University
1991-1997
University of Alabama at Birmingham
1997
The University of Texas Health Science Center at San Antonio
1997
The University of Texas Health Science Center at Houston
1997
Vanderbilt University
1984-1990
Natriuretic peptides, produced in the heart, bind to natriuretic peptide receptor A (NPRA) and cause vasodilation natriuresis important regulation of blood pressure. We here report that mice lacking a functional Npr1 gene coding for NPRA have elevated pressures hearts exhibiting marked hypertrophy with interstitial fibrosis resembling seen human hypertensive heart disease. Echocardiographic evaluation demonstrated compensated state systemic hypertension which cardiac dilatation are evident...
Natriuretic peptides (NPs), mainly produced in heart [atrial (ANP) and B-type (BNP)], brain (CNP), kidney (urodilatin), decrease blood pressure increase salt excretion. These functions are mediated by natriuretic peptide receptors A B (NPRA NPRB) having cytoplasmic guanylyl cyclase domains that stimulated when the bind ligand. more abundantly expressed receptor (NPRC or C-type) has a short domain without activity. NPRC is thought to act as clearance receptor, although it may have additional...
<h3>Background</h3> Natalizumab (NTZ), a monoclonal antibody to human α<sub>4</sub>β<sub>1</sub>/β<sub>7</sub> integrin, is an effective therapy for multiple sclerosis (MS), albeit associated with progressive multifocal leukoencephalopathy (PML). Clinicians have been extending the dose of infusions hypothesis reducing PML risk. The aim study evaluate clinical consequences NTZ frequency infusion up 8 weeks 5 days. <h3>Methods</h3> A retrospective chart review in 9 MS centres was performed...
The potent diuretic and natriuretic peptide hormone atrial factor (ANF), with vasodilatory activity also stimulates steroidogenic responsiveness in Leydig cells. actions of ANF are mediated by its interaction specific cell surface receptors the membrane-bound form guanylate cyclase represents an receptor (ANF-R). To understand mechanism action testicular steroidogenesis to identify cyclase/ANF-R that is expressed cells, primary structure murine has been deduced from cDNA sequence. A library...
Guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) plays a critical role in the regulation of blood pressure and fluid volume homeostasis. Mice lacking functional
Three distinct atrial natriuretic factor (ANF) receptors have been identified and characterized from rat thoracic aortic cultured vascular smooth muscle (RTASM) cells, kidney tubular epithelium (MDCK), Leydig tumor (MA-10) cells. These include 1) a disulfide-linked 140-kDa protein found in RTASM which was reduced by dithiothreitol (DTT) to 70 kDa, 2) 120-135-kDa single polypeptide protein, specific MDCK MA-10 cells whose Mr not DTT, 3) 66-70-kDa prevalent both DTT. After incubation of with...
Mice carrying a targeted disruption of the Npr1 gene (coding for guanylyl cyclase/natriuretic peptide receptor A (NPRA)) exhibit increased blood pressure, cardiac hypertrophy, and congestive heart failure, similar to untreated human hypertensive patients. The objective this study was determine whether permanent ablation NPRA signaling in mice alters expression matrix metalloproteinase (MMP)-2 MMP-9 pro-inflammatory mediators such as tumor necrosis factor-α (TNF-α), leading myocardial...
ADVERTISEMENT RETURN TO ISSUEPREVArticleAtrial natriuretic factor receptor on cultured Leydig tumor cells: ligand binding and photoaffinity labelingKailash N. Pandey, Tadashi Inagami, Kunio S. MisonoCite this: Biochemistry 1986, 25, 26, 8467–8472Publication Date (Print):December 1, 1986Publication History Published online1 May 2002Published inissue 1 December 1986https://pubs.acs.org/doi/10.1021/bi00374a022https://doi.org/10.1021/bi00374a022research-articleACS PublicationsRequest reuse...
The pioneering work of Dr Lewis K. Dahl established a relationship between kidney, salt, and high blood pressure (BP), which led to the major genetic-based experimental model hypertension. BP, heritable quantitative trait affected by numerous biological environmental stimuli, is cause morbidity mortality worldwide considered be primary modifiable factor in renal, cardiovascular, cerebrovascular diseases. Genome-wide association studies have identified monogenic polygenic variants affecting...
Renin and angiotensins coexist in various tissues. The mode of control the extrarenal renin-angiotensin system is not clear. Whether it renin or angiotensin that secreted has been identified. We have investigated gonadotropin-dependent synthesis subsequent release components intracellular a cloned cultured mouse Leydig tumor cell line (MA-10). Treatment cells with bovine luteinizing hormone (bLH, 100 ng/ml) human chorionic gonadotropin (hCG, 25 resulted greater than 150- 40- fold increased...
The objective of the present study was to delineate mechanisms GC-A/natriuretic peptide receptor-A (GC-A/NPRA) gene (<i>Npr1</i>) expression in vivo. We used all-trans retinoic acid (ATRA) and histone deacetylase (HDAC) inhibitor, sodium butyrate (NaBu) examine function <i>Npr1</i> using gene-disrupted heterozygous (1-copy; +/−), wild-type (2-copy; +/+), gene-duplicated (3-copy; ++/+) mice. <i>Npr1</i><b><sup>+/−</sup></b> mice exhibited increased renal HDAC reduced acetyltransferase (HAT)...
The objective of this study was to describe SymptoMScreen, an in-house developed tool for rapid assessment MS symptom severity in routine clinical practice, and validate SymptoMScreen against Performance Scales (PS). patients typically experience symptoms many neurologic domains. A that would enable efficiently relay their across multiple domains the healthcare providers could lead improved management. We "SymptoMScreen," a battery 7-point Likert scales 12 distinct commonly affected by MS:...
Atrial natriuretic peptide (ANP) binds guanylyl cyclase-A/natriuretic receptor-A (GC-A/NPRA) and produces the intracellular second messenger, cGMP, which regulates cardiovascular homeostasis. We sought to determine function of histone deacetylases (HDACs) in regulating Npr1 (coding for GC-A/NPRA) gene transcription, using primary mouse mesangial cells treated with class-specific HDAC inhibitors (HDACi). Trichostatin A, a pan inhibitor, mocetinostat (MGCD0103), class I significantly enhanced...
Mice lacking the gene (Npr1) encoding natriuretic peptide receptor A (NPRA) have hypertension with elevated blood pressure and cardiac hypertrophy. In particular, Npr1 gene-deficient male mice exhibit lethal vascular events similar to those seen in untreated human hypertensive patients. Serum testosterone levels tend be lower humans than normal males without hypertension, but genetic basis for this tendency remains unknown. To determine whether function affects level, we measured serum a...
We have investigated the mechanism by which different natriuretic peptides stimulate steroidogenesis in purified mouse Leydig cells. In addition to atrial factor (ANF), we show that brain peptide (BNP) and C-type (CNP) also testosterone production these Testosterone was increased dramatically 14-fold with ANF (EC50 = 0.3 nM) 15-fold BNP 0.2 nM); however, CNP-stimulated level of only 2.5-fold compared controls. enhanced stimulatory effect LH on production. The C-ANF(4-23) (a truncated form...
The deficiency of Npr1 [genetic determinant natriuretic peptide receptor A (NPRA)] increases arterial pressures and causes hypertensive heart disease in mice similar to those seen untreated human patients. However, the quantitative role NPRA mediating renal responses blood volume expansion remains uncertain. To determine specific contribution signaling mechanisms responsible for diuretic nondilutional intravascular expansion, we administered whole anesthetized homozygous null mutant...
We have studied cardiovascular and renal phenotypes in Npr1 (genetic determinant of natriuretic peptide receptor-A; NPRA) gene-disrupted mutant mouse model. The baseline systolic arterial pressure (SAP) 0-copy (−/−) mice (143 ± 2 mmHg) was significantly higher than 2-copy wild-type (+/+) animals (104 mmHg); however, the SAP 1-copy heterozygotes (+/−) at an intermediate value (120 4 mmHg). To determine whether gene function affects renin-angiotensin-aldosterone system (RAAS), we measured...
Binding of atrial and brain natriuretic peptides to guanylyl cyclase-A/natriuretic peptide receptor-A produces second messenger cGMP, which plays an important role in maintaining renal cardiovascular homeostasis. Mice carrying a targeted disruption the Npr1 gene coding for exhibit changes that are similar those occur untreated human hypertension, including elevated blood pressure, cardiac hypertrophy, congestive heart failure. The objective this study was determine whether mice provokes...