A5101431305- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Memory and Neural Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Regulation of Appetite and Obesity
- Nicotinic Acetylcholine Receptors Study
- Olfactory and Sensory Function Studies
- Adipose Tissue and Metabolism
- Tryptophan and brain disorders
- Sleep and Wakefulness Research
- Forensic Toxicology and Drug Analysis
- Congenital Anomalies and Fetal Surgery
- Heart Failure Treatment and Management
- Renal and related cancers
- Ion channel regulation and function
- Prenatal Screening and Diagnostics
- Receptor Mechanisms and Signaling
- Diet and metabolism studies
- Neuroendocrine regulation and behavior
- Adenosine and Purinergic Signaling
- Asthma and respiratory diseases
- Human Health and Disease
- Renin-Angiotensin System Studies
- Biochemical Analysis and Sensing Techniques
- Cardiac Fibrosis and Remodeling
National Institutes of Health
2013-2024
Jeju National University
2024
National Institute of Neurological Disorders and Stroke
2023-2024
University of North Carolina at Chapel Hill
1998-2022
University of North Carolina Health Care
2017
University of California, Irvine Medical Center
2016
National Institute on Alcohol Abuse and Alcoholism
2012-2013
Brookhaven National Laboratory
2010-2013
Stony Brook University
2010
Behavioral Pharma (United States)
2010
Natriuretic peptides, produced in the heart, bind to natriuretic peptide receptor A (NPRA) and cause vasodilation natriuresis important regulation of blood pressure. We here report that mice lacking a functional Npr1 gene coding for NPRA have elevated pressures hearts exhibiting marked hypertrophy with interstitial fibrosis resembling seen human hypertensive heart disease. Echocardiographic evaluation demonstrated compensated state systemic hypertension which cardiac dilatation are evident...
Dopamine D2 receptors are involved with wakefulness, but their role in the decreased alertness associated sleep deprivation is unclear. We had shown that reduced dopamine D2/D3 receptor availability (measured PET and [ 11 C]raclopride controls) striatum, could not determine whether this reflected increases ([ competes for binding) or downregulation. To clarify this, we compared induced by methylphenidate (a drug blocking transporters) during versus rested sleep, assumption methylphenidate's...
Overexpression of the amyloid precursor protein (APP) gene on chromosome 21 in Down syndrome (DS) has been linked to increased brain levels and early-onset Alzheimer’s disease (AD). An elderly man with phenotypic DS partial trisomy (PT21) lacked triplication APP affording an opportunity study role this pathogenesis dementia. Multidisciplinary studies between ages 66–72 years comprised neuropsychological testing, independent neurological exams, PET imaging 11 C-Pittsburgh compound-B (PiB),...
Neurons of the basal forebrain nucleus basalis and posterior substantia innominata (NBM/SIp) comprise major source cholinergic input to basolateral amygdala (BLA). Using a genetically encoded acetylcholine (ACh) sensor in mice, we demonstrate that BLA-projecting neurons can 'learn' association between naive tone foot shock (training) release ACh BLA response conditioned 24 hr later (recall). In NBM/SIp express immediate early gene, Fos following both training memory recall. Cholinergic...
Activation of the natriuretic peptide system lowers blood pressure and causes excretion salt. Atrial B-type are humoral mediators this effect; they act primarily by binding to membrane-bound receptor A (NPRA) stimulating its intrinsic guanylate cyclase activity. To study whether genetically determined differences in NPRA expression affect we have generated mice with one, two, three, or four copies gene encoding ( Npr1 mouse). peptide-dependent activity ranged progressively from approximately...
Methamphetamine (MA) addiction is a growing epidemic worldwide. Chronic MA use has been shown to lead neurotoxicity in rodents and humans. Magnetic resonance imaging (MRI) studies users have enlarged striatal volumes positron emission tomography (PET) decreased brain glucose metabolism (BGluM) the striatum of detoxified users. The present study examines structural changes brain, observes microglial activation, assesses function, response chronic treatment. Rats were randomly split into three...
To better understand the function of cholinergic projection neurons in ventral pallidum (VP), we examined behavioral responses to appetitive (APP) and aversive (AV) odors that elicited approach or avoidance, respectively. Exposure each odor increased cFos expression calcium signaling VP neurons. Activity Cre-dependent viral vectors selectively labeled were activated reactivated response either APP AV odors, but not both, identifying two non-overlapping populations differentially by valence...
Positron emission tomography (PET) with [(18)F]2-fluoro-2-deoxy-D-glucose was used to measure changes in regional brain glucose metabolism (BGluM) response optogenetic stimulation (using the excitatory channelrhodopsin-2) of nucleus accumbens (NAc) awake rats. We demonstrated not only increases BGluM that correlated c-Fos expression region stimulation, but also ipsilateral striatum, periaqueductal gray, and somatosensory cortex, contralateral amygdala, ventral pallidum, globus pallidus,...
Abstract Astrocytes play numerous vital roles in the central nervous system. Accordingly, it is of merit to identify structural and functional properties astrocytes both health disease. The majority studies examining morphology have employed immunoassays for markers such as glial fibrillary acidic protein, which are insufficient encapsulate considerable complexity these cells. Herein, we describe a method utilizing commercially available validated, genetically encoded membrane‐associated...
Abstract Accumulating evidence indicates significant consequences for astrocytes associated with drug abuse. For example, reductions in structural features and synaptic colocalization of male rat nucleus accumbens (NAc) are observed following short-access (ShA; 2 h/d) self-administration extinction from cocaine, methamphetamine, heroin. However, it is unknown whether these observations extend to other rodent models abuse, how enduring effects may be, similar female rats. Here, we assess the...
Abstract Birthweight is a marker for suboptimal fetal growth and development in utero . Offspring can be born large gestational age (LGA), which linked to maternal obesity or excessive weight gain, as well small (SGA), arising from nutrient calorie deficiency, placental dysfunction, other conditions (hypertension, infection). In humans, LGA SGA babies are at an increased risk certain neurodevelopmental disorders, including Attention Deficit/Hyperactivity Disorder, schizophrenia, social mood...
Abstract The ventral pallidum (VP) mediates motivated behaviors largely via the action of VP GABA and glutamatergic neurons. In addition to these neuronal subtypes, there is a population cholinergic projection neurons in VP, whose functional significance remains unclear. To understand role neurons, we first examined behavioral responses an appetitive (APP) odor that elicited approach, aversive (AV) led avoidance. examine how were engaged APP vs. AV responses, used immediate early gene marker...
Abstract Neurons of the basal forebrain nucleus basalis and posterior substantia innominata (NBM/SI p ) comprise major source cholinergic input to basolateral amygdala (BLA). Using a genetically-encoded acetylcholine (ACh) sensor in mice, we demonstrate that BLA-projecting neurons can “learn” association between naïve tone foot shock (training) release ACh BLA response conditioned 24h later (recall). In NBM/SI express immediate early gene, Fos following both training memory recall....