A5004888894- Memory and Neural Mechanisms
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- Neurotransmitter Receptor Influence on Behavior
- Functional Brain Connectivity Studies
- Neuroendocrine regulation and behavior
- Bipolar Disorder and Treatment
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
- Treatment of Major Depression
- Advanced Fluorescence Microscopy Techniques
- Attention Deficit Hyperactivity Disorder
- Neuroinflammation and Neurodegeneration Mechanisms
- Health Systems, Economic Evaluations, Quality of Life
- Tryptophan and brain disorders
- Olfactory and Sensory Function Studies
- Sleep and Wakefulness Research
- Cholinesterase and Neurodegenerative Diseases
- Stress Responses and Cortisol
- Neural dynamics and brain function
- Medical Imaging Techniques and Applications
- Generative Adversarial Networks and Image Synthesis
- Electroconvulsive Therapy Studies
- Lanthanide and Transition Metal Complexes
- Data Visualization and Analytics
National Institute of Neurological Disorders and Stroke
2021-2025
Yale University
2024
National Institutes of Health
2020-2024
Rutgers Sexual and Reproductive Health and Rights
2024
Emory University
2024
Stony Brook Medicine
2024
LinkedIn (United States)
2024
Kansas City University
2024
National Institute of Environmental Health Sciences
2024
Steris (United States)
2024
The ability to map the functional connectivity of discrete cell types in intact mammalian brain during behavior is crucial for advancing our understanding function normal and disease states. We combined designer receptor exclusively activated by drug (DREADD) technology behavioral imaging with μPET [18F]fluorodeoxyglucose (FDG) generate whole-brain metabolic maps cell-specific circuits awake, freely moving state. have termed this approach DREADD-assisted mapping (DREAMM) documented its rats...
Methamphetamine (MA) addiction is a growing epidemic worldwide. Chronic MA use has been shown to lead neurotoxicity in rodents and humans. Magnetic resonance imaging (MRI) studies users have enlarged striatal volumes positron emission tomography (PET) decreased brain glucose metabolism (BGluM) the striatum of detoxified users. The present study examines structural changes brain, observes microglial activation, assesses function, response chronic treatment. Rats were randomly split into three...
Neurons of the basal forebrain nucleus basalis and posterior substantia innominata (NBM/SIp) comprise major source cholinergic input to basolateral amygdala (BLA). Using a genetically encoded acetylcholine (ACh) sensor in mice, we demonstrate that BLA-projecting neurons can 'learn' association between naive tone foot shock (training) release ACh BLA response conditioned 24 hr later (recall). In NBM/SIp express immediate early gene, Fos following both training memory recall. Cholinergic...
To better understand the function of cholinergic projection neurons in ventral pallidum (VP), we examined behavioral responses to appetitive (APP) and aversive (AV) odors that elicited approach or avoidance, respectively. Exposure each odor increased cFos expression calcium signaling VP neurons. Activity Cre-dependent viral vectors selectively labeled were activated reactivated response either APP AV odors, but not both, identifying two non-overlapping populations differentially by valence...
Abstract Background Reported childhood adversity (CA) is associated with development of depression in adulthood and predicts a more severe course illness. Although elevated serotonin 1A receptor (5-HT R) binding potential, especially the raphe nuclei, has been shown to be trait major depression, we did not replicate this finding an independent sample using partial agonist positron emission tomography tracer [ 11 C]CUMI-101. Evidence suggests that CA can induce long-lasting changes expression...
Cocaine is a highly addictive drug of abuse for which there are currently no medications. In rats and mice d-cycloserine (DCS), partial NMDA agonist, accelerates extinction cocaine seeking behavior. Since cues delay here, we evaluated the effects in with without presence cues.Two doses DCS (15 30 mg/kg) were studied C57 mice. Mice self-administered (1 2 weeks then underwent 20-day period where was administered i.p. immediately following each daily session. Extinction conducted some...
Abstract Recent developments in the generation of neuronal population‐specific, genetically modified mouse lines have allowed precise identification and selective stimulation cholinergic neurons vivo . Although considerably less laborious than studies conducted with post hoc by immunostaining, it is not known whether based labeling procedures that permit are electrophysiologically benign. In this study, we use mice carrying a bacterial artificial chromosome transgene drives expression...
Wide-field microscopes are commonly used in neurobiology for experimental studies of brain samples. Available visualization tools limited to electron, two-photon, and confocal microscopy datasets, current volume rendering techniques do not yield effective results when with wide-field data. We present a workflow the neuronal structures images introduce novel gradient-based distance transform that overcomes out-of-focus blur caused by inherent design microscopes. This is followed extraction 3D...
Loudness dependence of auditory evoked potentials (LDAEP) has long been considered to reflect central basal serotonin transmission. However, the relationship between LDAEP and individual receptors transporters not fully explored in humans may involve other neurotransmitter systems. To examine LDAEP's with system, we performed PET using serotonin-1A (5-HT1A) imaging via [11C]CUMI-101 transporter (5-HTT) [11C]DASB on a mixed sample healthy controls (n = 4: 4 females, 0 males), patients...
Abstract Birthweight is a marker for suboptimal fetal growth and development in utero . Offspring can be born large gestational age (LGA), which linked to maternal obesity or excessive weight gain, as well small (SGA), arising from nutrient calorie deficiency, placental dysfunction, other conditions (hypertension, infection). In humans, LGA SGA babies are at an increased risk certain neurodevelopmental disorders, including Attention Deficit/Hyperactivity Disorder, schizophrenia, social mood...
We introduce NeuroConstruct, a novel end-to-end application for the segmentation, registration, and visualization of brain volumes imaged using wide-field microscopy. NeuroConstruct offers Segmentation Toolbox with various annotation helper functions that aid experts to effectively precisely annotate micrometer resolution neurites. It also an automatic neurites segmentation convolutional neuronal networks (CNN) trained by annotations somas thresholding. To visualize in given volume, hybrid...
Major depressive disorder (MDD) is a debilitating condition that affects over 14 million Americans. Remission occurs only in minority of individuals after first-line antidepressant treatment (∼35%); predictors outcome are therefore needed. Using PET imaging with radiotracer specific for the serotonin transporter (5-HTT), <sup>11</sup>C-McN5652, we found patients MDD who did not achieve remission 12 mo naturalistic had lower pretreatment midbrain and amygdala binding than healthy volunteers....
Abstract Introduction Novel environment stimulation is thought to have an important role in cognitive development and has been shown encourage exploratory behavior rats. However, psychopathology or perceived danger stress can impede this drive. The balance between brain circuits controlling the drive elicited by a novel environment, avoidance response stressors, not well understood. Methods Using positron emission tomography (PET) glucose analog [ 18 F]fluorodeoxyglucose (18F‐FDG), we...
Summary Although the engagement of cholinergic signaling in threat memory is well established (Knox, 2016a), our finding that specific neurons are requisite partners a engram likely to surprise many. Neurons basal forebrain nucleus basalis and substantia innominata (NBM/SI p ) comprise major source input basolateral amygdala (BLA), whose activation required for both acquisition retrieval cued innate response behavior. The by presentation conditioning tone alone elicits acetylcholine (ACh)...
Abstract The ventral pallidum (VP) mediates motivated behaviors largely via the action of VP GABA and glutamatergic neurons. In addition to these neuronal subtypes, there is a population cholinergic projection neurons in VP, whose functional significance remains unclear. To understand role neurons, we first examined behavioral responses an appetitive (APP) odor that elicited approach, aversive (AV) led avoidance. examine how were engaged APP vs. AV responses, used immediate early gene marker...