A5049026491- Memory and Neural Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- 3D Printing in Biomedical Research
- Neurotransmitter Receptor Influence on Behavior
- Attention Deficit Hyperactivity Disorder
- Functional Brain Connectivity Studies
- Liver physiology and pathology
- Neural dynamics and brain function
- Pharmacogenetics and Drug Metabolism
- Cholinesterase and Neurodegenerative Diseases
- Neuroscience and Neural Engineering
- Treatment of Major Depression
- Neuroendocrine regulation and behavior
- Innovative Teaching Methods
- Radio, Podcasts, and Digital Media
- Neuroinflammation and Neurodegeneration Mechanisms
- EEG and Brain-Computer Interfaces
- Digital Storytelling and Education
Yale University
2020-2024
LinkedIn (United States)
2024
National Institutes of Health
2024
Kansas City University
2024
National Institute of Environmental Health Sciences
2024
Steris (United States)
2024
Peking University
2024
University of New Haven
2022
Draper Laboratory
2012-2014
University of South Florida
2012
In this work, we describe the fabrication and working of a modular microsystem that recapitulates functions "Neurovascular Unit". The microdevice comprised vertical stack poly(dimethylsiloxane) (PDMS) neural parenchymal chamber separated by vascular channel via microporous polycarbonate (PC) membrane. housed mixture neurons (~4%), astrocytes (~95%), microglia (~1%). was lined with layer rat brain microvascular endothelial cell line (RBE4). Cellular components in showed viability (>90%)....
The basolateral amygdala (BLA) is critical for associating initially neutral cues with appetitive and aversive stimuli receives dense neuromodulatory acetylcholine (ACh) projections. We measured BLA ACh signaling activity of neurons expressing CaMKIIα (a marker glutamatergic principal cells) in mice during cue-reward learning using a fluorescent sensor calcium indicators. found that levels nucleus basalis Meynert (NBM) cholinergic terminal the (NBM-BLA) increased sharply response to...
Neurons of the basal forebrain nucleus basalis and posterior substantia innominata (NBM/SIp) comprise major source cholinergic input to basolateral amygdala (BLA). Using a genetically encoded acetylcholine (ACh) sensor in mice, we demonstrate that BLA-projecting neurons can 'learn' association between naive tone foot shock (training) release ACh BLA response conditioned 24 hr later (recall). In NBM/SIp express immediate early gene, Fos following both training memory recall. Cholinergic...
While many advanced liver models support hepatic phenotypes necessary for drug and disease studies, these are characterized by intricate features such as co-culture with one of more supporting cell types or media perfusion systems. These systems have helped elucidate some the critical biophysical missing from standard well-plate based hepatocyte culture, but their designs add to complexity. Additionally, regardless culture system, primary suffer reproducibility issues due phenotypic...
Summary Although the engagement of cholinergic signaling in threat memory is well established (Knox, 2016a), our finding that specific neurons are requisite partners a engram likely to surprise many. Neurons basal forebrain nucleus basalis and substantia innominata (NBM/SI p ) comprise major source input basolateral amygdala (BLA), whose activation required for both acquisition retrieval cued innate response behavior. The by presentation conditioning tone alone elicits acetylcholine (ACh)...
The Flipped Science Fair (FSF) transforms the traditional science fair format by having middle-school students judge research of early career scientists. At FSF, learn about cutting-edge in a small group setting, with opportunities to ask questions and participate hands-on demonstrations. By placing role "judge," event gives opportunity engage scientists interactively authority. FSF also provides communication training for presenting Leading up event, presenters attend three workshops...
Abstract Patients with focal temporal lobe seizures often experience loss of consciousness. In humans, this consciousness has been shown to be positively correlated EEG neocortical slow waves, similar those seen in non-REM sleep. Previous work rat models suggests that decreased activity subcortical arousal systems cause depressed cortical function during seizures. However, these studies were performed under light anesthesia, making it impossible correlate behavior, and therefore...
Increased brain levels of acetylcholine (ACh) are observed in subsets patients with depression and increasing ACh chronically can precipitate stress-related behaviors humans animals. Conversely, optimal required for cognition memory. We hypothesize that signaling is important encoding both appetitive stress-relevant memories, but excessive increases result a negative bias which memory formation stressful event aberrantly strengthened, potentially contributing to the focus on experience could...
Abstract Neurons of the basal forebrain nucleus basalis and posterior substantia innominata (NBM/SI p ) comprise major source cholinergic input to basolateral amygdala (BLA). Using a genetically-encoded acetylcholine (ACh) sensor in mice, we demonstrate that BLA-projecting neurons can “learn” association between naïve tone foot shock (training) release ACh BLA response conditioned 24h later (recall). In NBM/SI express immediate early gene, Fos following both training memory recall....
Although the engagement of cholinergic signaling in threat memory is well established (Knox, 2016a), our finding that specific neurons are requisite partners a engram likely to surprise many. Neurons basal forebrain nucleus basalis and substantia innominata (NBM/SI p ) comprise major source input basolateral amygdala (BLA), whose activation required for both acquisition retrieval cued innate response behavior. The by presentation conditioning tone alone elicits acetylcholine (ACh) release...
Abstract The basolateral amygdala (BLA) is critical for associating initially neutral cues with appetitive and aversive stimuli receives dense neuromodulatory acetylcholine (ACh) projections. We measured BLA ACh signaling principal neuron activity in mice during cue-reward learning using a fluorescent sensor calcium indicators. found that levels of nucleus basalis Meynert (NBM) cholinergic terminals the (NBM-BLA) increased sharply response to reward-related events shifted as learned...