A5101855774- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cell Image Analysis Techniques
- Electrowetting and Microfluidic Technologies
- Advanced Fluorescence Microscopy Techniques
- Cancer-related gene regulation
- Adenosine and Purinergic Signaling
- Wound Healing and Treatments
- Cancer Treatment and Pharmacology
- Optical Coherence Tomography Applications
- Axon Guidance and Neuronal Signaling
- Neuropeptides and Animal Physiology
- Lattice Boltzmann Simulation Studies
- Chronic Kidney Disease and Diabetes
- Microbial Natural Products and Biosynthesis
- Cell Adhesion Molecules Research
- Botanical Studies and Applications
- Virus-based gene therapy research
- Complement system in diseases
- Hereditary Neurological Disorders
- Microfluidic and Bio-sensing Technologies
- Inflammasome and immune disorders
- Fluid Dynamics and Heat Transfer
Hong Kong University of Science and Technology
2019-2025
University of Hong Kong
2019-2025
Shanghai East Hospital
2022-2023
Baoding University
2022
Harbin Medical University
2021
Third Affiliated Hospital of Harbin Medical University
2021
Henan University
2019
Wenzhou Medical University
2018
Zhejiang Taizhou Hospital
2018
Abstract Single-molecule localization microscopy (SMLM) can be used to resolve subcellular structures and achieve a tenfold improvement in spatial resolution compared that obtained by conventional fluorescence microscopy. However, the separation of single-molecule events requires thousands frames dramatically increases image acquisition time phototoxicity, impeding observation instantaneous intracellular dynamics. Here we develop deep-learning based single-frame super-resolution (SFSRM)...
Abstract Microfluidic droplets, with their unique properties and broad applications, are essential in chemical, biological, materials synthesis research. Despite the flourishing studies on artificial intelligence‐accelerated microfluidics, most research efforts have focused upstream design phase of microfluidic systems. Generating user‐desired droplets still remains laborious, inefficient, time‐consuming. To address long‐standing challenges associated accurate efficient identification,...
Breast cancer has become the leading cause of global incidence and a serious threat to women's health. Accurate diagnosis early treatment are great importance prognosis. Although clinically used diagnostic approaches can be for screening, accurate breast is still critical unmet need. Here, we report 4-plex droplet digital PCR technology simultaneous detection four small extracellular vesicle (sEV)-derived mRNAs (PGR, ESR1, ERBB2 GAPDH) in combination with machine learning (ML) algorithms...
FAS and its ligand FASL are crucial in apoptotic cell death. Loss of gain aberrant expression common features malignant transformation. This study was designed to investigate whether the functional polymorphisms -1377G/A (rs2234767) -844T/C (rs763110) affect risk developing breast cancer. Genotypes were analyzed by a polymerase chain reaction-restriction fragment length polymorphism assay 436 cancer patients 496 healthy controls. In this study, as compared wild-type homozygote heterozygote,...
Spexin/NPQ is a novel highly conserved neuropeptide. It has widespread expression in the periphery and central nervous system. However, effects of spexin on acute inflammatory pain are still unknown. This study explored mechanisms supraspinal pain. The results from mouse formalin test show that i.c.v. administration decreased licking/biting time during late early phases. nonamidated had no effect response. antinociception was blocked by galanin receptor 3 antagonist SNAP 37889. Galr3 Adcy4...
Drug resistance represents a major obstacle to improving the overall response and survival of cancer patients. Taxol is one most commonly used chemotherapy agents in breast cancer. As with many therapeutic agents, remains significant problem when using treat malignancies. In this study, estrogen receptor positive cells MCF‐7 were induced resistance. And Tanshinone IIA combined was chosen it. The drugs combination showed additive effect drug concentrations. higher microtubule associated...
Blood-brain-barrier (BBB) serves as a fatal guard of the central nervous system well formidable obstacle for treatment brain diseases such tumors. Cell membrane-derived nanomedicines are promising drug carriers to achieve BBB-penetrating and lesion targeting. However, challenge precise size control has severely limited their therapeutic effect clinical application in diseases. To address this problem, work develops microfluidic mixing platform that enables fabrication cell nanovesicles with...
Droplet microfluidics enables the generation of highly uniform emulsions with excellent stability, precise control over droplet volume, and morphology, which offer superior platforms conventional technologies for material synthesis biological assays. However, it remains a challenge to scale up production microfluidic devices due their complicated geometry long-term reliability. In this study, we present high-throughput generator by parallelization high aspect ratio rectangular structures,...
Abstract Single-molecule localization microscopy (SMLM) can be used to resolve subcellular structures and achieve a tenfold improvement in spatial resolution compared that obtained by conventional fluorescence microscopy. However, the separation of single-molecule events thousands frames dramatically increases image acquisition time phototoxicity, impeding observation instantaneous intracellular dynamics. Based on deep learning networks, we develop single-frame super-resolution (SFSRM)...
Elsholtzia stauntonii Benth. (Lamiaceae) is an economically important ornamental, medicinal and aromatic plant species. To meet the increasing market demand for E. , it necessary to assess genetic diversity within species accelerate process of improvement. Analysis transferability simple sequence repeat (SSR) markers from related or genera a fast economical method evaluate diversity, can ensure availability molecular in crops with limited genomic resources. In this study, cross-genera 497...
Wound healing is a programmed process through which tissue restores its integrity after an injury. Advancing age risk factor for delayed cutaneous wound healing; however, ideal therapeutic approaches aged have not been developed yet. By dissecting the harsh microenvironment of wound, we propose integrated chemical and biological strategy to mitigate two main hostile factors including oxidative stress ischemia. Mesenchymal stem cell-derived extracellular vesicles (EVs) are rising star in...
The dysregulation of the microRNAs, small noncoding RNA around 22 bases, accounts for a wide range diseases including cancer. However, current methods technologies microRNA detection and analysis are limited to bulk exosomes, which fails depict heterogeneity exosome population as individual vesicles. In this work, we developed reverse transcription polymerase chain reaction (RT-PCR) protocol on droplet microfluidic platform. We employed synthetic hsa-miR-21-5p exosomes extracted from lung...
Mixed lineage leukemia 1 (MLL1) is a methyltransferase that induces histone H3 lysine 4 trimethylation (H3K4me3) and partially exerts its untoward functional effects by interacting with multiple subunits including menin WD repeat-containing protein 5 (WDR5). In this study, we investigated the role mechanisms of MLL1 in murine models acute kidney injury induced folic acid (FA) ischemia-reperfusion. Injury to elevated expression MLL1, menin, WDR5, H3K4Me3, which was accompanied increased serum...
Abstract Background: The left-sided and right-sided colon adenocarcinoma (LCCs RCCs, respectively) have unique characteristics in various aspects, particularly molecular features clinical heterogeneity. purpose of our study was to develop a prognostic risk model based on differential genomic instability-associated (DGIA) Long non-coding RNAs (lncRNAs) LCCs therefore the key lncRNAs could be identified. Methods: We adopted two independent gene data-sets, corresponding somatic mutation...
Objective: To construct a recombinant short-hairpin RNA (shRNA) lentiviral vector targeting the β-catenin gene in cochlear precursor cells (CPCs) mice, and to investigate its inhibitory effect. Methods: PCR was used for multiplication of gene, shRNA oligo designed based on an interference vector. Gateway Technology which carried then 293FT were transfected with constructed helper plasmids pLV/helper-SL3, pLV/helper-SL4, pLV/helper-SL5. The virus supernatant collected obtain viral particles,...