A5088818017- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Optical Coherence Tomography Applications
- Image Processing Techniques and Applications
- Single-cell and spatial transcriptomics
- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Congenital heart defects research
- Advanced Electron Microscopy Techniques and Applications
- Robotic Path Planning Algorithms
- Hydrogels: synthesis, properties, applications
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Lymphoma Diagnosis and Treatment
- Cellular Mechanics and Interactions
- Spectroscopy and Chemometric Analyses
- Electrowetting and Microfluidic Technologies
- Genetics, Aging, and Longevity in Model Organisms
- Spectroscopy Techniques in Biomedical and Chemical Research
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Pediatric Hepatobiliary Diseases and Treatments
- Laser Applications in Dentistry and Medicine
- Biosensors and Analytical Detection
Huazhong University of Science and Technology
2016-2025
Zhangzhou Normal University
2022-2025
Duke University
2025
Second Affiliated Hospital of Guangzhou Medical University
2025
Guangzhou Medical University
2025
Wuhan National Laboratory for Optoelectronics
2017-2024
Nanchang University
2024
Tongji Hospital
2008-2024
Guangdong University of Technology
2018-2022
Wuhan Children's Hospital
2021-2022
Abstract Single-molecule localization microscopy (SMLM) can be used to resolve subcellular structures and achieve a tenfold improvement in spatial resolution compared that obtained by conventional fluorescence microscopy. However, the separation of single-molecule events requires thousands frames dramatically increases image acquisition time phototoxicity, impeding observation instantaneous intracellular dynamics. Here we develop deep-learning based single-frame super-resolution (SFSRM)...
Combining information from multispectral images into a fused image is informative and beneficial for human or machine perception. Currently, multiple photodetectors with different response bands are used, which require complicated algorithms systems to solve the pixel position mismatch problem. An ideal solution would be pixel-level fusion, involves using same photodetector circumventing Here we presented potential of fusion utilizing colloidal quantum dots photodiode array, broadband range...
Abstract Rapid 3D imaging of entire organs and organisms at cellular resolution is a recurring challenge in life science. Here we report on computational light-sheet microscopy able to achieve minute-timescale high-resolution mapping macro-scale organs. Through combining dual-side confocally-scanned Bessel illumination which provides thinner-and-wider optical sectioning deep tissues, with content-aware compressed sensing (CACS) computation pipeline further improves the contrast based single...
Solution-processed colloidal quantum dots (CQDs) are promising candidates for broadband photodetectors from visible light to shortwave infrared (SWIR). However, large-size PbS CQDs sensitive longer SWIR mainly exposed with nonpolar (100) facets on the surface, which lack robust passivation strategies. Herein, an innovative strategy that employs planar cation, is introduced enable face-to-face coupling and strengthen halide (111) facets. The defect density of film (E
Hemodynamic shear force has been implicated as modulating Notch signaling-mediated cardiac trabeculation. Whether the spatiotemporal variations in wall stress (WSS) coordinate initiation of trabeculation to influence ventricular contractile function remains unknown. Using light-sheet fluorescent microscopy, we reconstructed 4D moving domain and applied computational fluid dynamics quantify WSS along trabecular ridges groves. In WT zebrafish, pulsatile developed ridges, with prominent...
An optofluidic device interfaces droplet microfluidics with light-sheet fluorescence microscopy for on-chip, high-throughput sample compartmentalization and 3-D imaging.
A key challenge when imaging whole biomedical specimens is how to quickly obtain massive cellular information over a large field of view (FOV). We report subvoxel light-sheet microscopy (SLSM) method enabling high-throughput volumetric mesoscale at resolution. nonaxial, continuous scanning strategy developed rapidly acquire stack large-FOV images with three-dimensional (3-D) nanoscale shifts encoded. Then, by adopting subvoxel-resolving procedure, the SLSM models these low-resolution,...
Recent studies show that NK cells play important roles in murine biliary atresia (BA), and a temporary immunological gap exists this disease. In study, we found high-mobility group box-1 (HMGB1) TLRs were overexpressed human rotavirus-induced BA. The HMGB1 released from the nuclei of rotavirus-infected cholangiocytes, as well macrophages, activated hepatic via HMGB1-TLRs-MAPK signaling pathways. Immature had low cytotoxicity on rotavirus-injured cholangiocytes due to expression TLRs, which...
Abstract Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination rapidly uncover architecture of murine hearts over 10 by mm 3 volume; thereby allowing for localizing progenitor differentiation cardiomyocyte lineage AAV9-mediated expression exogenous transmembrane potassium channels high contrast...
This study sought to develop an automated segmentation approach based on histogram analysis of raw axial images acquired by light-sheet fluorescent imaging (LSFI) establish rapid reconstruction the 3-D zebrafish cardiac architecture in response doxorubicin-induced injury and repair. Input underwent a 4-step image process consisting stationary noise removal, equalization, adaptive thresholding, fusion followed reconstruction. We applied this method 3-month old injected intraperitoneally with...
Escape is an evolutionarily conserved and essential avoidance response. Considered to be innate, most studies on escape responses focused hard-wired circuits. We report here that a neuropeptide NLP-18 its cholecystokinin receptor CKR-1 enable the circuit execute full omega (Ω) turn. demonstrate in vivo mainly secreted by gustatory sensory neuron (ASI) activate head motor (SMD) turn-initiating interneuron (AIB). Removal of or specific knockdown SMD AIB neurons leads shallower turns, hence...
Light sheet microscopy combined with a microchip is an emerging tool in biomedical research that notably improves efficiency. However, microchip-enhanced light-sheet limited by noticeable aberrations induced the complex refractive indices chip. Herein, we report droplet specifically engineered to be capable of large-scale culture 3D spheroids (over 600 samples per chip) and has polymer index matched water (difference <1%). When lab-built open-top microscope, this technique allows time-lapse...
In this paper, we investigate how the order in which tokens are unmasked during masked diffusion models (MDMs) inference affects generative quality. We derive an expanded evidence lower bound (ELBO) that introduces a planner, responsible for selecting to unmask at each step. Our analysis suggests alternative unmasking strategies can improve performance. Based on these insights, propose Path Planning (P2), sampling framework leverages pre-trained BERT or denoiser itself guide decisions. P2...