A5062873812- Parathyroid Disorders and Treatments
- Bone Metabolism and Diseases
- Dermatological and Skeletal Disorders
- Bone health and treatments
- Bone and Dental Protein Studies
- Lipoproteins and Cardiovascular Health
- Cardiac Imaging and Diagnostics
- Cardiac Valve Diseases and Treatments
- Coronary Interventions and Diagnostics
- Bone health and osteoporosis research
- Connective tissue disorders research
- Vitamin D Research Studies
- Cellular Mechanics and Interactions
- Atherosclerosis and Cardiovascular Diseases
- Caveolin-1 and cellular processes
- Aortic Disease and Treatment Approaches
- Biomarkers in Disease Mechanisms
- Aortic aneurysm repair treatments
- Cell Adhesion Molecules Research
- Heterotopic Ossification and Related Conditions
- Cancer, Lipids, and Metabolism
- Alkaline Phosphatase Research Studies
- Antioxidant Activity and Oxidative Stress
- Peroxisome Proliferator-Activated Receptors
- Advanced MRI Techniques and Applications
University of California, Los Angeles
2015-2025
Oklahoma State University Center for Health Sciences
1994-2024
Bioengineering Center
2015-2024
UCLA Health
2004-2024
VA Greater Los Angeles Healthcare System
2021-2023
La Jolla Bioengineering Institute
2011-2019
Matrix Research (United States)
2001-2016
Samueli Institute
2016
Ronald Reagan UCLA Medical Center
2015
Lourdes University
2015
Abstract
Artery wall calcification associated with atherosclerosis frequently contains fully formed bone tissue including marrow. The cellular origin is not known. In this study, morphogenetic protein-2a, a potent factor for osteoblastic differentiation, was found to be expressed in calcified human atherosclerotic plaque. addition, cells cultured from the aortic nodules similar those cell cultures and protein-2a prolonged culture. predominant these had immunocytochemical features characteristic of...
Abstract Atherosclerotic calcification and osteoporosis often coexist in patients, yielding formation of bone mineral vascular walls its simultaneous loss from bone. To assess the potential role lipoproteins both processes, we examined effects minimally oxidized low-density lipoprotein (MM-LDL) several other lipid oxidation products on calcifying cells (CVCs) bone-derived preosteoblasts MC3T3-E1. In CVCs, MM-LDL but not native LDL inhibited proliferation, caused a dose-dependent increase...
Background —Vascular calcification is an ectopic that commonly occurs in atherosclerosis. Because tumor necrosis factor-α (TNF-α), a pleiotropic cytokine found atherosclerotic lesions, also regulator of bone formation, we investigated the role TNF-α vitro vascular calcification. Methods and Results —A cloned subpopulation bovine aortic smooth muscle cells previously shown capable osteoblastic differentiation was treated with TNF-α, mineralization were assessed. Treatment for 3 days induced...
Arterial calcification is a common feature of atherosclerosis, occurring in >90% angiographically significant lesions. Recent evidence from this and other studies suggests that development atherosclerotic similar to osteogenesis; thus, we undertook the current investigation on potential role osteoregulatory factors arterial calcification.We studied two human populations (173 subjects) at high moderate risk for coronary heart disease assessed them associations between vascular serum levels...
Previous studies in our laboratory demonstrated messenger RNA for bone morphogenetic protein-2a human calcified plaque, suggesting that arterial calcification is a regulated process, similar to osteogenesis. To further test this hypothesis, we have isolated and cloned subpopulation of cells from bovine aortic media show osteoblastic potential. These novel are primarily distinguished smooth muscle by expression surface marker preliminarily identified as modified form the ganglioside...
Peroxisome proliferator-activated receptor-gamma (PPARgamma) is activated by fatty acids, eicosanoids, and insulin-sensitizing thiazolidinediones (TZDs). The TZD troglitazone (TRO) inhibits vascular smooth muscle cell (VSMC) proliferation migration in vitro postinjury intimal hyperplasia.Rat human VSMCs express mRNA nuclear receptors for PPARgamma1. Three PPARgamma ligands, the TZDs TRO rosiglitazone prostanoid 15-deoxy-Delta(12,14)-prostaglandin J2 (15d-PGJ2), all inhibited VSMC migration....
T cell cytokines are known to play a major role in determining protection and pathology infectious disease. It has recently become clear that IL-12 is key inducer of the type 1 cytokine pattern characterized by production IFN-gamma. Conversely, IL-10 down-regulates responses. We have investigated whether might be involved chronic inflammatory reaction, atherosclerosis. In atherosclerotic plaques, we found strong expression IFN-gamma but not IL-4 mRNAs as compared normal arteries. p40 mRNA...
Abstract —An early event in acute and chronic inflammation associated diseases such as atherosclerosis rheumatoid arthritis is the induced expression of specific adhesion molecules on surface endothelial cells (ECs), which subsequently bind leukocytes. Peroxisome proliferator–activated receptors (PPARs), members nuclear receptor superfamily transcription factors, are activated by fatty acid metabolites, peroxisome proliferators, thiazolidinediones now recognized important mediators...
Background— In diabetes or atherosclerosis, ectopic bone, fat, cartilage, and marrow often develop in arteries. However the mechanism is unknown. We have previously identified a subpopulation of vascular cells (calcifying cells, CVC), derived by dilutional cloning bovine aortic medial showed that they undergo osteoblastic differentiation mineralization. now show CVC potential to differentiate along other mesenchymal lineages. Methods Results— To determine multilineage CVC, molecular...
Calcification is a common complication of atherosclerosis and other chronic inflammatory processes that involves infiltration monocytes accumulation macrophages.To determine whether these cells modulate vascular calcification in vitro, calcifying (CVCs), subpopulation osteoblast-like derived from the artery wall, were cocultured with human peripheral blood for 5 days. Results showed alkaline phosphatase (ALP) activity, marker osteoblastic differentiation, was significantly greater cocultures...
To assess the accuracy of positron emission tomography (PET) for evaluation coronary artery disease (CAD), cardiac PET perfusion images were obtained at rest and with dipyridamole-handgrip stress in 193 patients undergoing arteriography. reviewed by two independent readers blinded to clinical data. Subjective defect severity scores assigned each myocardial region on a 0 (normal) 5 (severe) scale. Results compared arteriographic stenosis expressed as flow reserve (SFR), continuous values...
Abstract —Leptin, the product of ob gene, regulates food intake, energy expenditure, and other physiological functions peripheral tissues. Leptin receptors have been identified in hypothalamus extrahypothalamic Increased circulating leptin levels correlated with cardiovascular disease, obesity, aging, infection bacterial lipopolysaccharide, high-fat diets. All these conditions also increased vascular calcification, a hallmark atherosclerotic age-related disease. In addition, differentiation...
Abstract The epidemiological correlation between osteoporosis and cardiovascular disease is independent of age, but the basis for this unknown. We previously found that atherogenic oxidized lipids inhibit osteoblastic differentiation in vitro ex vivo, suggesting an diet may contribute to both diseases. In study, effects high-fat versus control chow on bone were tested two strains mice with genetically different susceptibility atherosclerosis lipid oxidation. After 4 months 7 diets, mineral...
In osteoporosis, the bone marrow stroma osteogenic cell population declines and adipocyte numbers increase. We recently showed that oxidized lipids inhibit differentiation of preosteoblasts. this report, we assess effect minimally low density lipoprotein (MM-LDL) on osteoblastic murine stromal cells, M2-10B4. MM-LDL, but not native LDL, inhibited as demonstrated by inhibition alkaline phosphatase activity, collagen I processing, mineralization, through a mitogen-activated protein...
Background— The role of osteoprotegerin in vascular disease is unclear. Recent observational studies show that serum levels are associated with the severity and progression coronary artery disease, atherosclerosis, calcification patients. However, genetic treatment mice suggest may protect against calcification. Methods Results— To test whether induces or prevents we treated atherogenic diet–fed ldlr (−/−) recombinant (Fc-OPG) vehicle for 5 months. Vehicle-treated developed significant,...