A5101783356- Advanced Fluorescence Microscopy Techniques
- Nanoplatforms for cancer theranostics
- Luminescence and Fluorescent Materials
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Optical Imaging and Spectroscopy Techniques
- Photonic and Optical Devices
- Environmental Toxicology and Ecotoxicology
- Cell Image Analysis Techniques
- Molecular Sensors and Ion Detection
- Traumatic Brain Injury and Neurovascular Disturbances
- Marine Biology and Environmental Chemistry
- Photodynamic Therapy Research Studies
- Pulmonary Hypertension Research and Treatments
- Advanced Photonic Communication Systems
- Photonic Crystals and Applications
- Porphyrin and Phthalocyanine Chemistry
- Digital Holography and Microscopy
- Cerebrospinal fluid and hydrocephalus
- Sulfur Compounds in Biology
- Laser Applications in Dentistry and Medicine
- Neuroinflammation and Neurodegeneration Mechanisms
- Effects and risks of endocrine disrupting chemicals
- Carbon and Quantum Dots Applications
- Advanced Sensor and Energy Harvesting Materials
Wuhan National Laboratory for Optoelectronics
2020-2025
Nanjing Medical University
2024-2025
Jiangsu Province Hospital
2024-2025
Huazhong University of Science and Technology
2016-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Wuhan University
2025
Fuzhou University
2024
China Medical University
2014-2024
Institute of Biophysics
2020-2024
Collaborative Innovation Center of Chemistry for Energy Materials
2024
Currently, a serious problem obstructing the large-scale clinical applications of fluorescence technique is shallow penetration depth. Two-photon microscopic imaging with excitation in longer-wavelength near-infrared (NIR) region (>1100 nm) and emission NIR-I (650–950 good choice to realize deep-tissue high-resolution imaging. Here, we report ultradeep two-photon bioimaging 1300 nm NIR-II (peak ∼810 based on NIR aggregation-induced luminogen (AIEgen). The crab-shaped AIEgen possesses planar...
Abstract Stimulated emission depletion (STED) nanoscopy is a typical super‐resolution imaging technique that has become powerful tool for visualizing intracellular structures on the nanometer scale. Aggregation‐induced (AIE) luminogens are ideal fluorescent agents bioimaging. Herein, long‐term fluorescence of cancer cells, based STED assisted by AIE nanoparticles (NPs) realized. 2,3‐Bis(4‐(phenyl(4‐(1,2,2‐triphenylvinyl)phenyl)amino)phenyl) fumaronitrile (TTF), luminogen, doped into...
Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep sectioning, high-spatial-resolution imaging, and 3D reconstruction biological specimens. Developing organic fluorescent probes with strong nonlinear effects, particular third-harmonic generation (THG), is promising for exploiting microscopic imaging biomedical applications. Herein, simple method preparing nanocrystals based on an aggregation-induced emission (AIE) luminogen...
The development of fluorogens with deep-red emission is one the hottest topics investigation in field bio/chemosensors and bioimaging. Herein, tunable fluorescence perylene diimide (PDI) derivatives was achieved by incorporation varied isolation groups linked on PDI core. With enlarged sizes groups, conversion from aggregation caused quenching to aggregation-induced obtained their variations solutions nanoparticles, as result efficient inhibition π-π stacking larger groups. Accordingly,...
We propose and experimentally demonstrate a novel ultracompact dual-mode waveguide crossing based on subwavelength multimode-interference couplers for densely integrated on-chip mode-division multiplexing system. By engineering the lateral-cladding material index manipulating phase profiles of light at nanoscale using an improved inverse design method, structure could theoretically realize identical beat length both TE0 TE1, which can reduce scale device greatly. The fabricated occupied...
Organic fluorescent dyes with high quantum yield are widely applied in bioimaging and biosensing. However, most of them suffer from a severe effect called aggregation-caused quenching (ACQ), which means that their fluorescence is quenched at molecular concentrations or the aggregation state. Aggregation-induced emission (AIE) diametrically opposite phenomenon to ACQ, luminogens this feature can effectively solve problem. Graphene oxide has been utilized as quencher for many dyes, based on...
Inverse-designed free-form nanophotonic structures have shown great potential in designing ultra-compact integrated photonic devices, but strict fabrication requirements may hinder further applications. We propose here a photonic-crystal-like (PhC-like) subwavelength structure, which is insensitive to the lag effect that most common error. A colorless 3 dB coupler employing such structure designed, fabricated, and characterized. With only one-step etching, coupling region of our final device...
Abstract Intraventricular hemorrhage is one of the most fatal forms brain injury that a common complication premature infants. However, therapy this type limited, and new strategies are needed to reduce hematoma expansion. Here we show meningeal lymphatics pathway remove red blood cells from brain’s ventricular system male human, adult newborn rodents target for non-invasive transcranial near infrared photobiomodulation. Our results uncover clinical significance phototherapy intraventricular...
Aggregation-induced emission nanoparticles, TPE-red–PSMA, were prepared and used as photosensitizers for two-photon excited photodynamic therapy under 1040 nm fs laser excitation.
Laser speckle contrast imaging (LSCI) is a powerful tool to monitor blood flow distribution and has been widely used in studies of microcirculation, both for animal clinical applications. Conventionally, LSCI usually works on reflective-detected mode. However, it could provide promising temporal spatial resolution vivo applications only with the assistance various tissue windows, otherwise, overlarge superficial static would extremely limit its resolution. Here, we systematically...
Abstract Modern optical imaging techniques provide powerful tools for observing cortical structure and functions at high resolutions. Various skull windows have been established different applications of imaging, each has its advantages limitations. Most critical the limitations, none current is suitable responses to some acute craniocerebral injuries on a large scale resolution. Here, we developed “Through-Intact-Skull (TIS) window” that enables observation an immune response bilateral...
The brain diseases account for 30% of all known diseases. Pharmacological treatment is hampered by the blood–brain barrier, limiting drug delivery to central nervous system (CNS). Transcranial photobiomodulation (tPBM) a promising technology treating diseases, due its effectiveness, non-invasiveness, and affordability. tPBM has been widely used in pre-clinical experiments clinical trials such as stroke Alzheimer's disease. This review provides comprehensive overview tPBM. We summarize...
A new near-infrared fluorescence off-on probe was developed and applied to imaging of carboxylesterase in living HepG-2 cells zebrafish pretreated with pesticides (carbamate, organophosphorus, pyrethroid). The readily prepared by connecting (4-acetoxybenzyl)oxy as a quenching recognizing moiety stable hemicyanine skeleton that can be formed via the decomposition IR-780. response is based on enzyme-catalyzed spontaneous hydrolysis carboxylic ester bond, followed further fragmentation...
With the development of highly densified photonic integrated circuits, optical cross nodes number exhibits dramatically increasing. Not only efficient but also ultra-compact waveguide crossings are required to materialize full potential silicon photonics for on-chip intercross connect. In this work, we proposed several inverse-designed 4 × 4, 5 and 6 star-crossings based on photonic-crystal-like (PhC-like) subwavelength structures, which have ultra-high port density about 7.1 μm2/port, 5.83...
The optical/electronic properties of organic luminescent materials can be regulated by molecular structure modification, which not only requires sophisticated and time-consuming synthesis but also is unable to accurately afford the optical in aggregate state. Herein, a facile strategy synergistic engineering proposed manipulate luminogen, ACIK, solid state for efficient diversified functions. ACIK facilely synthesized exhibits three polymorphic states (ACIK-Y, ACIK-R, ACIK-N) with large...
Cryo-electron tomography (cryo-ET) is widely used to explore the 3D density of biomacromolecules. However, heavy noise and missing wedge effect prevent directly visualizing analyzing reconstructions. Here, we introduced REST, a deep learning strategy-based method establish relationship between low-quality high-quality transfer knowledge restore signals in cryo-ET. Test results on simulated real cryo-ET datasets show that REST performs well denoising compensating information. The application...
Efficient labeling of the vasculature is important for understanding organization vascular networks. Here, we propose VALID, a vessel-labeling method that enables visualization networks with tissue clearing and light-sheet microscopy. VALID transforms traditional lipophilic dye solution into hydrogel by introducing gelatin restrains aggregation, resulting in complete uniform patterns high signal-to-background ratios. also enhances compatibility dyes solvent-based tissue-clearing protocols,...
Light sheet microscopy combined with a microchip is an emerging tool in biomedical research that notably improves efficiency. However, microchip-enhanced light-sheet limited by noticeable aberrations induced the complex refractive indices chip. Herein, we report droplet specifically engineered to be capable of large-scale culture 3D spheroids (over 600 samples per chip) and has polymer index matched water (difference <1%). When lab-built open-top microscope, this technique allows time-lapse...