A5083489446- Pancreatic function and diabetes
- COVID-19 Clinical Research Studies
- Metabolism, Diabetes, and Cancer
- Diabetes and associated disorders
- RNA modifications and cancer
- Opportunistic and Delay-Tolerant Networks
- Microtubule and mitosis dynamics
- Inflammasome and immune disorders
- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
- Long-Term Effects of COVID-19
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Extracellular vesicles in disease
- Age of Information Optimization
- Endoplasmic Reticulum Stress and Disease
- Human Mobility and Location-Based Analysis
- Mechanisms of cancer metastasis
- Platelet Disorders and Treatments
- Protein Kinase Regulation and GTPase Signaling
- Pancreatic and Hepatic Oncology Research
- Hemophilia Treatment and Research
- Heme Oxygenase-1 and Carbon Monoxide
- Trauma, Hemostasis, Coagulopathy, Resuscitation
Academy of Military Medical Sciences
2019-2025
Czech Academy of Sciences, Institute of Biotechnology
2021
Beijing VDJBio (China)
2020
University Town of Shenzhen
2017
Tsinghua University
2017
University of Otago
2015-2016
Viral infection triggers the formation of mitochondrial antiviral signaling protein (MAVS) aggregates, which potently promote immune signaling. Autophagy plays an important role in controlling MAVS-mediated signaling; however, exact molecular mechanism underlying targeted autophagic degradation MAVS remains unclear. Here, we investigated by RNF34 regulates immunity and mitophagy targeting MAVS. binds to compartment after viral negatively RIG-I-like receptor (RLR)-mediated immunity. Moreover,...
The tumor acidic microenvironment, a common biochemical event in solid tumors, offers evolutional advantage for tumors cells and even enhances their aggressive phenotype. However, little is known about the molecular mechanism underlying microenvironment-induced invasion metastasis.We examined expression of acid-sending ion channel (ASIC) family members after exposure using RT-PCR immunofluoresence. Gene manipulation was applied to reveal potential ASIC2 on invasion, proliferation, colony...
Abstract In this study, we specifically addressed the connection between SARS-CoV-2 virus with host cholesterol metabolism. Plasma lipid profile was measured in 861 COVID-19 patients classified as mild (n=215), moderate (n=364), severe (n=217) or critical (n=65) and 1108 age- sex-matched healthy individuals. We showed that levels of both TG HDL-C were significantly lower disease than disease. After successful treatment, metabolism reestablished infection. The serum concentrations TC can be...
Immunotherapy that targets immune checkpoints has achieved revolutionary success, but its application in solid tumors remains limited, highlighting the need for reliable enhancement of efficacy immunotherapy. Golgi protein 73 (GP73), a membrane protein, been implicated various cellular processes, including regulation. Recent studies suggested GP73 may play role modulating response patients with cancer. In this study, we investigated mechanism by which regulates T-cell-mediated antitumor...
Coronavirus disease 2019 (COVID-19) is characterized by fever, fatigue, dry cough, dyspnea, mild pneumonia and acute lung injury (ALI), which can lead to respiratory distress syndrome (ARDS), SARS-CoV-2 accelerate tumor progression. However, the molecular mechanism for increased mortality in cancer patients infected with COVID-19 unclear. Colony formation wound healing assays were performed on Huh-7 cells cocultured syncytia. Exosomes purified from cell supernatant verified nanoparticle...
Abstract The prevalence of non-obese nonalcoholic fatty liver disease (NAFLD) is increasing worldwide with unclear etiology and pathogenesis. Here, we show GP73, a Golgi protein upregulated in livers from patients variety diseases, exhibits Rab GTPase-activating (GAP) activity regulating ApoB export. Upon regular-diet feeding, liver-GP73-high mice display NAFLD phenotype, characterized by reduced body weight, intrahepatic lipid accumulation, gradual insulin resistance development, none which...
Recent reports have shown the critical role of mitochondrial antiviral signaling (MAVS) protein in virus-induced apoptosis, but involvement MAVS tumorigenesis is still poorly understood. Herein, we report that a key regulator p53 activation and for protecting against tumorigenesis. We find promotes p53-dependent cell death response to DNA damage. interacts with mediates recruitment under genotoxic stress. Mechanistically, inhibits ubiquitination by blocking formation p53-murine double-minute...
Activation of the NLRP3 inflammasome requires expression NLRP3, which is strictly regulated by its capacity to directly recognize microbial-derived substances. Even though involvement caspase-1 activation in macrophages via and IPAF has been discovered, accurate mechanisms Shigella infection triggers remain inadequately understood. Here, we demonstrate that IpaH4.5, a T3SS effector, regulating through E3 ubiquitin ligase activity IpaH4.5. First, found IpaH4.5 interacted with NLRP3. As...
Inflammation play important roles in the initiation and progression of acute lung injury (ALI), respiratory distress syndrome (ARDS), septic shock, clotting dysfunction, or even death associated with SARS-CoV-2 infection. However, pathogenic mechanisms underlying SARS-CoV-2-induced hyperinflammation are still largely unknown. The animal model shock ALI was established after LPS intraperitoneal injection intratracheal instillation. Bone marrow-derived macrophages (BMDMs) from WT BPOZ-2 KO...
Introduction. Shigella flexneri is an intracellular bacterial pathogen that utilizes a type III secretion apparatus to inject effector proteins into host cells. Hypothesis/Gap Statement. The T3SS IpaH4.5 important for the virulence of . Aim. This study aimed elucidate molecular mechanism and target as well its roles in S. infection. Methodology. GAP assay was used identify substrate Rab GTPases IpaH4.5. A coimmunoprecipitation applied interaction with confocal microscopy analysis assess...
Golgi protein 73 (GP73), also called membrane 1 (GOLM1), is a resident type II transmembrane and considered as serum marker for the detection of variety cancers. A recent work revealed role secreted GP73 in stimulating liver glucose production systemic homeostasis. Since exaggerated hepatic plays key pathogenesis diabetes mellitus (T1DM) 2 (T2DM), may thus represent potential therapeutic target treating diabetic patients with pathologically elevated levels. Here, this study, we found that...
Heparanase, an endo-glucuronidase that specifically cleaves heparan sulfate (HS), is upregulated in several pathological conditions. In this study, we aimed to find a correlation of heparanase expression and platelets production. the transgenic mice overexpressing human (Hpa-tg), hematological analysis blood samples revealed significantly higher number comparison with wild-type (Ctr) mice, while no significant difference was found leukocytes red cell between two groups. Total thiazole orange...
Pancreatic cancer (PC), characterized by aggressive local invasion and metastasis, is one of the most malignant cancers. Gemcitabine currently used as standard drug for treatment advanced metastatic PC, but with limited efficacy. In this study, we demonstrated that gemcitabine increased expression heparanase (HPA1), only known mammalian endoglycosidase capable cleaving heparan sulfate, both in vitro vivo. Furthermore, overexpression HPA1 PC cell lines enhanced proliferation invasion,...
Abstract The recently emerged pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly, leading to a global COVID-19 pandemic. Binding of the viral spike protein (SARS-2-S) cell surface receptor angiotensin-converting enzyme (ACE2) mediates host infection. In present study, we demonstrate that in addition ACE2, S1 subunit SARS-2-S binds HDL and SARS-CoV-2 hijacks SR-B1-mediated uptake pathway facilitate its entry. SR-B1 facilitates entry into permissive...
Delay Tolerant Networking (DTN) is a promising technology that aims to provide efficient communication between devices in network with no guaranteed continuous connectivity.Most existing routing schemes for DTNs exploit the advantage of message replication achieve high delivery rate.However, these commonly suffer from large overhead due lack mechanisms control replication.In this paper we give brief survey on protocols designed DTNs, and evaluate performance several representative including...
SARS-CoV-2 spike protein (SARS-2-S) induced cell–cell fusion in uninfected cells may occur long COVID-19 syndrome, as circulating SARS-2-S or extracellular vesicles containing (S-EVs) were found to be prevalent post-acute sequelae of (PASC) for up 12 months after diagnosis. Although isolated recombinant has been shown increase the SASP senescent ACE2-expressing cells, direct linkage syncytia with senescence absence virus infection and degree which affect pathology setting cardiac dysfunction...
Pairwise contacts in Delay-Tolerant Networks (DTNs) for applications such as bus or smartphone based social networking commonly show some regular repeating patterns. Most existing routing protocols only implicitly exploit these patterns to predict future contacts. To enhance message delivery rate, most of the schemes allow messages be replicated and forwarded encountered nodes. However, there is no efficient mechanism dynamically controlling replication achieve high rate with very low...
Summary Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection induces new-onset diabetes and severe metabolic complications of pre-existing diabetes. The pathogenic mechanism underlying this is incompletely understood. Here, we provided evidence linking circulating GP73 with the exaggerated gluconeogenesis triggered by SARS-CoV-2 infection. We found that or glucotoxic condition increased cellular secretion GP73. Secreted trafficked to liver kidney stimulate through cAMP/PKA...
Recent reports discovered that red blood cells (RBCs) could scavenge cell-free mitochondrial DNA (mtDNA), which drives the accelerated erythrophagocytosis and innate immune activation characterized by anemia inflammatory cytokine production. However, clinical value of circulating mtDNA copy number alterations in hematologic malignancies is poorly understood. Our data showed comparison to healthy group, patients group had significantly higher histone H4 levels. Moreover, we observed RBC-bound...
Abstract Patients with pre-existing heart failure are at a particularly high risk of morbidity and mortality resulting from SARS-CoV-2 infection. Direct acute cardiac injury or cytokine storms have been proposed to contribute depressed function. However, the pathogenic mechanisms underlying increased vulnerability in infected patients still largely unknown. Here, we found that senescent outcome spike protein (SARS-2-S)-induced syncytia exacerbated progression. We first demonstrated syncytium...