A5101435569- interferon and immune responses
- Invertebrate Immune Response Mechanisms
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Biochemical and Structural Characterization
- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Antimicrobial Peptides and Activities
- Ubiquitin and proteasome pathways
- Healthcare and Venom Research
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Protease and Inhibitor Mechanisms
- RNA modifications and cancer
- Bone and Joint Diseases
- Neurobiology and Insect Physiology Research
- Calpain Protease Function and Regulation
- Chemical Synthesis and Analysis
- Pancreatic and Hepatic Oncology Research
- Text and Document Classification Technologies
- Inflammasome and immune disorders
- Plant Molecular Biology Research
- Insect symbiosis and bacterial influences
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer-related molecular mechanisms research
Anhui Agricultural University
2022-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2023
Nanjing Medical University
2018
Kaohsiung Armed Forces General Hospital
2012
Abstract AR-23 is a melittin-related peptide with 23 residues. Like melittin, its high α-helical amphipathic structure results in strong bactericidal activity and cytotoxicity. In this study, series of analogues low amphipathicity were designed by substitution Ala1, Ala8 Ile17 positively charged residues (Arg or Lys) to study the effect residue distribution on biological viability antimicrobial peptide. Substitution nonpolar face Lys dramatically altered hydrophobicity, amphipathicity,...
Significance Upon virus infection, host cells detect viral nucleic acids and initiate antiviral innate immune responses by producing type I IFNs proinflammatory cytokines. As a cytoplasmic sensor for RNA viruses, retinoic acid-inducible gene 1 (RIG-I) recognizes the pathogenic RNAs activates downstream signaling initiation of immunity. In this study, we identify RING finger protein 122 (RNF122) as an E3 ligase that directly binds to RIG-I induce K48-linked ubiquitination mediate degradation,...
Cullin 2 (Cul2), a core component of the Cullin-RING E3 ubiquitin ligase complex, is integral to regulating distinct biological processes. However, its role in innate immune defenses remains poorly understood. In this study, we investigated functional significance Cul2 deficiency (IMD) signaling-mediated antimicrobial reactions Drosophila melanogaster (fruit fly). We demonstrated that loss-of-function led marked reduction peptide induction following bacterial infection, which was associated...
Negative regulators of the inflammatory responses are essential for maintenance immune homeostasis and organismal fitness. In Drosophila , deubiquitinase (Dub) dTrbd selectively restricts K63-linked ubiquitination modification dTak1, a pivotal kinase IMD signaling pathway, to regulate innate response. However, which domain how it functions enable dTrbd’s activity remain unexplored. Here, we provide compelling evidence showing that NZF is its association with dTak1. Meanwhile, Linker region...
Mouse PUMILIO1 (PUM1) and PUMILIO2 (PUM2) belong to the PUF (Pumilio/FBF) family, a highly conserved RNA binding protein family whose homologues play critical roles in embryonic development germ line stem cell maintenance invertebrates. However, their mammalian remained largely uncharacterized. Here we report an essential requirement of Pum gene early development. A loss both Pum1 Pum2 genes led gastrulation failure, resulting embryo lethality at E8.5. Pum-deficient blastocysts, however,...
Acute myocardial infarction is a highly prevalent cardiovascular disease in Taiwan. Among several etiological risk factors, obesity and inflammation are strongly associated with the frequency of hypertension, disease, diabetes, infarction. To discriminate obesity- inflammation-related genes onset acute (AMI), case-control study was conducted to investigate association -308G/A polymorphisms tumor necrosis factor (TNF)-α C825T polymorphism guanidine nucleotide binding protein 3 (GNB3) AMI...
Heparanase (HPSE), the only known mammalian endoglycosidase responsible for heparan sulfate cleavage, is a multi-faceted protein affecting multiple malignant behaviors in cancer cells. In this study, we examined expression of HPSE different colorectal (CRC) cell lines. Gene manipulation was applied to reveal effect on proliferation, invasion and metastasis CRC. Knockdown resulted decreased proliferation vitro, whereas overexpression opposite phenomenon. Consistently, vivo data showed that...
Background: Drosophila ubiquitin carboxy-terminal hydrolase L5 (Uch-L5) functions as a critical component of the 26S proteasome to mediate degradation polyubiquitinated proteins. It was recently shown modulate tissue/organ development by targeting Smoothened protein in hedgehog pathway. However, whether it plays role controlling organismal immune response remains largely unknown. Methods: Reverse transcription plus quantitative polymerase chain reaction (RT-qPCR), dual-luciferase, and...
The highly conserved RNA binding protein PUF (Pumilio/FBF) family is present throughout eukaryotes from yeast to mammals, with critical roles in development, fertility and the nervous system. However, function of mammalian members remains underexplored. Our previous study reported that a gene-trap mutation Pum2 results smaller testis but does not impact viability. Although disrupted key functional domain PUM protein–PUM-HD (Pumilio homology domain), still produced chimeric Pum2-β-geo...
Pancreatic cancer (PC), characterized by aggressive local invasion and metastasis, is one of the most malignant cancers. Gemcitabine currently used as standard drug for treatment advanced metastatic PC, but with limited efficacy. In this study, we demonstrated that gemcitabine increased expression heparanase (HPA1), only known mammalian endoglycosidase capable cleaving heparan sulfate, both in vitro vivo. Furthermore, overexpression HPA1 PC cell lines enhanced proliferation invasion,...
We propose XRDMatch, a semi-supervised learning framework that integrates consistency regularization and pseudo-labeling. Using X-ray diffraction patterns as descriptors, it effectively addresses data scarcity by leveraging abundant unlabeled data.