A5027980684- Circular RNAs in diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Sperm and Testicular Function
- Metabolism and Genetic Disorders
- Genomics and Chromatin Dynamics
- ATP Synthase and ATPases Research
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
Zhujiang Hospital
2024
Southern Medical University
2024
Nanjing Medical University
2016-2022
University of Science and Technology of China
2020
Circular RNAs (circRNAs) are a large family of newly identified transcripts, and their physiological roles evolutionary significance require further characterization. Here, we identify circRNAs generated from conserved reproductive gene, Boule, in species Drosophila to humans. Flies missing circular Boule (circBoule) display decreased male fertility, sperm circBoule knockout mice exhibit fertilization capacity, when under heat stress conditions. During spermatogenesis, fly interact with...
Mouse PUMILIO1 (PUM1) and PUMILIO2 (PUM2) belong to the PUF (Pumilio/FBF) family, a highly conserved RNA binding protein family whose homologues play critical roles in embryonic development germ line stem cell maintenance invertebrates. However, their mammalian remained largely uncharacterized. Here we report an essential requirement of Pum gene early development. A loss both Pum1 Pum2 genes led gastrulation failure, resulting embryo lethality at E8.5. Pum-deficient blastocysts, however,...
Separation of germ cells from somatic is a widespread feature animal sexual reproduction, with core set cell factors conserved among diverse animals. It not known what controls their gonad-specific expression. Core components epigenetic machinery are ancient, but its role in tissue expression regulation remains unexplored. We found that promoters the reproductive genes BOULE and DAZL exhibit differential DNA methylation, consistent humans mice. Low or little promoter methylation testicular...
Abstract Testis size determination is an important question of reproductive biology. Sertoli cells are known to be a key determinant mammalian testis but the underlying molecular mechanisms remain incompletely understood. Previously we showed that highly conserved germ cell RNA-binding proteins, PUMILIO1(PUM1) and PUMILIO2 (PUM2), control mouse organ body through translational regulation, how different types organs contribute their regulation has not been established. Here, report somatic...