A5058107447- Neurobiology and Insect Physiology Research
- Insect Utilization and Effects
- Insect and Arachnid Ecology and Behavior
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Oxidative Organic Chemistry Reactions
- Plant Molecular Biology Research
- Catalytic C–H Functionalization Methods
- Plant Reproductive Biology
- Insect-Plant Interactions and Control
- Hippo pathway signaling and YAP/TAZ
- Traditional Chinese Medicine Studies
- Plant Virus Research Studies
- Sleep and Wakefulness Research
- Insect Pest Control Strategies
- Plant-Microbe Interactions and Immunity
- Phytochemical compounds biological activities
- S100 Proteins and Annexins
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- Plant Stress Responses and Tolerance
- Wnt/β-catenin signaling in development and cancer
- Circadian rhythm and melatonin
- Viral Infectious Diseases and Gene Expression in Insects
- Synthesis and Biological Evaluation
Chinese Academy of Sciences
2001-2025
Institute of Zoology
2001-2025
Chinese Institute for Brain Research
2024-2025
Peking University
2024-2025
Center for Life Sciences
2024-2025
University of Chinese Academy of Sciences
2021-2025
Sichuan Agricultural University
2022-2025
Capital Medical University
2022-2025
McGovern Institute for Brain Research
2025
Hunan Agricultural University
2024
Dysregulation of eating behavior can lead to obesity, which affects 10% the adult population worldwide and accounts for nearly 3 million deaths every year. Despite this burden on society, we currently lack effective pharmacological treatment options regulate appetite. We used Drosophila melanogaster larvae develop a high-throughput whole organism screen drugs that modulate food intake. In 3630 small molecules, identified serotonin (5-hydroxytryptamine or 5-HT) receptor antagonist metitepine...
With the completion of genome sequences major model organisms, increasingly sophisticated genetic tools are necessary for investigating complex and coordinated functions genes. Here we describe a manipulation system termed "genomic engineering" in Drosophila. Genomic engineering is 2-step process that combines ends-out (replacement) gene targeting with phage integrase phiC31-mediated DNA integration. First, through an improved modified method, founder knock-out line generated by deleting...
The Hippo signaling pathway regulates organ size and tissue homeostasis from Drosophila to mammals. At the core of is a kinase cascade extending (Hpo) tumor suppressor Yorkie (Yki) oncoprotein. cascade, in turn, regulated by apical membrane-associated proteins such as FERM domain Merlin Expanded (Ex), WW- C2-domain protein Kibra. How these are themselves remains poorly understood. Here, we identify transmembrane Crumbs (Crb), determinant epithelial apical-basal polarity embryos, an upstream...
Our understanding of the molecular mechanisms underlying sleep homeostasis is limited. We have taken a systematic approach to study neural signaling by transmitter 5-hydroxytryptamine (5-HT) in drosophila. generated knockout and knockin lines for Trh, 5-HT synthesizing enzyme all five receptors, making it possible us determine their expression patterns investigate functional roles. Loss 5HT1a or 5HT2b gene decreased time whereas loss Trh diminished rebound after deprivation. small subset of,...
An nBu4NI-catalyzed benzylic C–H acyloxylation of alkylarenes with readily available aromatic aldehydes has been developed. These reactions occur under mild and clean reaction conditions using tert-butyl hydroperoxide as the green terminal oxidant.
Abstract Polar auxin transport (PAT) plays a crucial role in the regulation of many aspects plant growth and development. We report characterization semidominant Arabidopsis thaliana bushy dwarf1 (bud1) mutant. Molecular genetic analysis indicated that bud1 phenotype is result increased expression MAP KINASE KINASE7 (MKK7), member mitogen-activated protein kinase group D. showed BUD1/MKK7 functional activity essential for its biological functions. Compared with wild type, plants develop...
The LH/CG receptor (LH/CG-R) belongs to the family of glycoprotein hormone G protein-coupled receptors. extracellular domain LH/CG-R is associated with high ligand-binding affinity and contains leucine-rich repeats (LRRs). With goal identifying essential amino acid residues involved in ligand binding, we replaced several conserved ionizable rat ones opposite charge. expression these mutants was assessed by binding studies Western blots after COS-7 cells were transiently transfected wild type...
Abstract In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets targeting vectors fly stocks introduced a novel negative selection marker that drastically reduced frequency false-positive candidates.
nBu4NI-catalyzed C3-selective formylation of N–H and N-substituted indoles by using N-methylaniline as a formylating reagent was first successfully demonstrated.
Lethal giant larvae (Lgl) plays essential and conserved functions in regulating both cell polarity tumorigenesis Drosophila melanogaster vertebrates. It is well recognized that plasma membrane (PM) or cortex localization crucial for Lgl function vivo, but its membrane-targeting mechanisms remain poorly understood. Here, we discovered hypoxia acutely reversibly inhibits PM targeting through a posttranslational mechanism independent of the well-characterized atypical protein kinase C (aPKC)...
Auxin-dependent cell expansion is crucial for initiation of fiber cells in cotton (Gossypium hirsutum), which ultimately determines yield and quality. However, the regulation this process far from being well understood. In study, we demonstrate an antagonistic effect between cytokinin (CK) auxin on initiation. vitro planta experiments indicate that enhanced CK levels can reduce accumulation ovule integument, may account defects fiberless mutant xu142fl. turn, supplementation with recover...
Independent of the design life cycle any insect, their growth and reproduction are highly choreographed through action two versatile hormones: ecdysteroids juvenile hormones (JH). However, means by which JH can target tissues exert its pleiotropic physiological effects is currently still not completely elucidated. Although identity one receptor elusive, recent evidence seems to point product Methoprene-tolerant gene (Met) as most likely contender in transducing JH. Studies on role this...
Juvenile hormones (JHs) are key regulators of insect development and reproduction. The JH biosynthetic pathway is known to involve 13 discrete enzymatic steps. In the present study, we have characterized in cockroach Diploptera punctata. effect exogenous precursors on biosynthesis was also determined. Based sequence similarity, orthologs for genes directly involved were cloned, their spatial temporal transcript profiles shutting down adult female cockroaches studied by knocking encoding...
Circular RNAs (circRNAs) are a large family of newly identified transcripts, and their physiological roles evolutionary significance require further characterization. Here, we identify circRNAs generated from conserved reproductive gene, Boule, in species Drosophila to humans. Flies missing circular Boule (circBoule) display decreased male fertility, sperm circBoule knockout mice exhibit fertilization capacity, when under heat stress conditions. During spermatogenesis, fly interact with...
Illicium sesquiterpenes are a large family of biologically active secondary metabolites isolated from species plants and well-known for their activity neurite outgrowth in cultured neurons. Herein, we propose comprehensive biosynthetic pathway illicium report synthetic route to illisimonin A merrilactone based on it. We think that the carbon scaffolds most could be synthesized dicarbonyl derivative allo-cedrane through retro-Dieckmann condensation, oxidative cleavage aldol reaction at...
An enzyme of molecular weight 32,000 comprising a single subunit has been isolated from whole cell extracts the yeast Saccharomyces cerevisiae. In vitro, transfers gamma phosphate ATP to protein substrate, histone H4, produce an alkali-stable phosphorylation. Modification substrate histidine with diethylpyrocarbonate prevented Phosphoamino acid analysis phosphorylated showed presence 1-phosphohistidine. Hence, is kinase. A novel assay for acid-labile phosphorylation was used in purification...
Adherens junctions (AJs) in epithelial cells are constantly turning over to modulate adhesion properties under various physiological and developmental contexts, but how such AJ dynamics regulated during the apical–basal polarization of primary epithelia remains unclear. Here, we used new genetically validated GFP markers Drosophila E-cadherin (DE-cadherin, hereafter referred as DE-Cad) β-catenin (Armadillo, Arm) quantitatively assay vivo biosynthetic turnover membrane redistribution by...