A5030985788- Research in Cotton Cultivation
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
- Plant Surface Properties and Treatments
- Mycotoxins in Agriculture and Food
- Nitrogen and Sulfur Effects on Brassica
- Toxin Mechanisms and Immunotoxins
- Plant biochemistry and biosynthesis
- Plant Genetic and Mutation Studies
- Polysaccharides and Plant Cell Walls
- Plant pathogens and resistance mechanisms
- Nuclear Structure and Function
- Advanced Cellulose Research Studies
- Plant Stress Responses and Tolerance
- Advanced Fluorescence Microscopy Techniques
Southwest University
2014-2024
Chongqing Academy of Agricultural Sciences
2024
Bioactive gibberellins (GAs) comprise an important class of natural plant growth regulators and play essential roles in cotton fiber development. To date, the molecular base GAs' functions development is largely unclear. address this question, endogenous bioactive GA levels developing fibers were elevated by specifically up-regulating 20-oxidase suppressing 2-oxidase via transgenic methods. Higher significantly increased micronaire values, 1000-fiber weight, cell wall thickness cellulose...
Cotton fibers are seed trichomes that make cotton unique compared with other plants. At anthesis, IAA, a major auxin in plants, accumulates the fiber cell to promote initiation. However, many important aspects of this process not clear. Here, distribution patterns indicated by auxin-dependent DR5::GUS (β-glucuronidase) expression ovules were studied during differentiation and initiation [-2 2 DPA (days post-anthesis)]. The nucellus two sites where accumulates. accumulation started from -1...
Auxin-dependent cell expansion is crucial for initiation of fiber cells in cotton (Gossypium hirsutum), which ultimately determines yield and quality. However, the regulation this process far from being well understood. In study, we demonstrate an antagonistic effect between cytokinin (CK) auxin on initiation. vitro planta experiments indicate that enhanced CK levels can reduce accumulation ovule integument, may account defects fiberless mutant xu142fl. turn, supplementation with recover...
Both abiotic and biotic stress factors adversely affect plant survival, biomass generation, crop yields. As the global availability of arable land decreases impacts warming intensify, such stressors may have increasingly pronounced effects on agricultural productivity. Currently, researchers face overarching challenge comprehensively enhancing resilience to stressors. The secondary cell wall plays a crucial role in bolstering resistance plants. To increase through genetic manipulation wall,...
Gibberellins (GAs) promote secondary cell wall (SCW) development in plants, but the underlying molecular mechanism is still to be elucidated. Here, we employed a new system, first internode of cotton, and virus-induced gene silencing method address this problem. We found that knocking down major DELLA genes via VIGS phenocopied GA treatment significantly enhanced SCW formation xylem phloem cotton stems. Cotton proteins were interact with wide range SCW-related NAC proteins, these inhibited...
Cotton fibre is a single cell that derived from the ovule epidermis. Fibre initiation and elongation determine cotton yield quality. Sucrose most important substrate for cellulose synthesis energy production, as well provides turgor pressure to promote elongation. However, varieties with high-sugar content in fibres usually have short fibres, mechanism behind this unclear. Sugar transporter SUTs (sucrose transport proteins) SWEETs (Sugars Will Eventually be Exported Transporters), which are...
Abstract Many toxic secondary metabolites produced by phytopathogens can subvert host immunity, and some of them are recognized as pathogenicity factors. Fusarium head blight Verticillium wilt destructive plant diseases worldwide. Using toxins the causal fungi graminearum dahliae screening agents, here we show that Arabidopsis P4 ATPases AtALA1 AtALA7 responsible for cellular detoxification mycotoxins. Through AtALA1-/AtALA7-mediated vesicle transport, sequestered in vacuoles degradation....
Abstract Cytokinin is considered to be an important driver of seed yield. To increase the yield cotton while avoiding negative consequences caused by constitutive overproduction cytokinin, we down-regulated specifically carpel genes for cytokinin oxidase/dehydrogenase (CKX), a key regulator levels, in transgenic cotton. The carpel-specific down-regulation CKXs significantly enhanced levels carpels. elevated promoted expression carpel- and ovule-development-associated genes, GhSTK2, GhAG1,...
Cotton fibers are single cells that show a relatively independent developmental process of cell differentiation, elongation, and secondary wall deposition. Auxin promotes fiber protrusion from the surface ovule. However, role auxin at other stages cotton development remains largely unknown. To gain deeper insight into this aspect, we measured indoleacetic acid (IAA) content in developing fibers. Results showed an increase IAA transition stage elongation to Subsequently, investigated...
Cotton fiber is a raw material for the global textile industry and quality essential to its industrial application. Carotenoids are plant secondary metabolites that may serve as dietary components, regulate light harvesting, scavenge reactive oxygen species. Although carotenoids accumulate predominantly in rapidly elongating cotton fibers, their roles development remain poorly understood. In this study, fiber-specific promoter proSCFP was applied drive expression of GhOR1Del, positive...
Cotton fibers are differentiated ovule epidermal cells that provide an ideal model to study cell differentiation and elongation. Establishment of auxin maximum in fiber is crucial for cotton-fiber protrusion from surface. However, it unclear where the originates how accumulates cells. Our recent results indicate mainly imported outside ovules, transported through GhPIN (homolog PIN-formed proteins cotton) -mediated polar transport, rather than situ synthesis. Based on our finding GhPINs, we...
Plant architecture and the vegetative-reproductive transition have major impacts on agronomic success of crop plants, but genetic mechanisms underlying these traits in cotton (Gossypium spp.) not been identified. We identify four natural mutations GoCEN-Dt associated with cluster fruiting (cl) early maturity. The situ hybridization shows that GhCEN is preferentially expressed shoot apical meristems (SAM) main stem axillary buds. Constitutive GhCEN-Dt overexpression suppresses vegetative apex...
Abstract PIN-FORMED- (PIN) mediated polar auxin transport plays a predominant role in most auxin-triggered organogenesis plants. Global control of PIN polarity at the plasma membrane contributes to essential establishment maxima multicellular tissues. However, single cells is poorly understood. Cotton fibers, derived from ovule epidermal by cell protrusion, provide an ideal model explore underlying mechanism. Here, we report that cell-specific degradation GhPIN3a, which guides gradient...
Cotton fiber is the leading natural textile material, and elongation plays an essential role in formation of cotton yield quality. Although a wealth components molecular network controlling have been reported, lot players still need to be functionally dissected understand regulatory mechanism comprehensively. In present study, R2R3-MYB transcription factor gene, GhMYB201, was characterized verified via CRISPR/Cas9 mediated gene editing. GhMYB201 homologous Arabidopsis AtMYB60, both coding...
Cotton fiber is the leading natural textile material, and elongation plays an essential role in formation of cotton yield quality. Although a number components molecular network controlling have been reported, lot players still need to be functionally dissected understand regulatory mechanism comprehensively. In present study, R2R3-MYB transcription factor gene, GhMYB201, was characterized verified via CRISPR/Cas9-mediated gene editing. GhMYB201 homologous Arabidopsis AtMYB60, both coding...
Four P4-ATPase flippase genes, VdDrs2, VdNeo1, VdP4-4, and VdDnf1 were identified in Verticillium dahliae, one of the most devastating phytopathogenic fungi world. Knock out or knock down significantly decreased pathogenicity mutants cotton. Among mutants, greatest decrease was observed ΔVdDrs2. VdDrs2 localized to plasma membrane, vacuoles, trans-Golgi network (TGN). In vivo observation showed that infection cotton by ΔVdDrs2 delayed. The amount two known toxins, sulfacetamide, fumonisin B1...
Verticillium wilt, caused by dahliae, is a devastating disease for many important crops, including cotton. Kiwellins (KWLs), group of cysteine-rich proteins synthesized in plants, have been shown to be involved response various phytopathogens. To evaluate genes their function resistance we investigated KWL homologs Thirty-five (GhKWLs) were identified from the genome upland cotton (Gossypium hirsutum). Among them, GhKWL1 was localized nucleus and cytosol, its gene expression induced...