A5079869058- Research in Cotton Cultivation
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Refrigeration and Air Conditioning Technologies
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Advanced Thermodynamic Systems and Engines
- Plant nutrient uptake and metabolism
- Transgenic Plants and Applications
- Entomopathogenic Microorganisms in Pest Control
- Studies on Chitinases and Chitosanases
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Toxin Mechanisms and Immunotoxins
- Fungal and yeast genetics research
- Advanced Sensor Technologies Research
- Plant Gene Expression Analysis
- Heat Transfer and Boiling Studies
- Click Chemistry and Applications
- Nematode management and characterization studies
- Agriculture, Soil, Plant Science
- Insect symbiosis and bacterial influences
- Geoscience and Mining Technology
- Biochemical and Structural Characterization
- GNSS positioning and interference
Southwest University
2014-2024
Biotechnology Research Center
2023
Southwest University of Science and Technology
2017-2021
National University of Defense Technology
2015
Summary Cotton ( Gossypium hirsutum L.) fibers, one of the most important natural raw materials for textile industry, are highly elongated trichomes from epidermal cells cotton ovules. DET2, an Arabidopsis steroid 5d‐reductase, is considered to catalyze a major rate‐limiting in brassinosteroid (BR) biosynthesis. To understand role BRs fiber development, GhDET2 , which putatively encodes 5α‐reductase by sequence comparison, was cloned developing cells. In vitro assessment protein activity...
Bioactive gibberellins (GAs) comprise an important class of natural plant growth regulators and play essential roles in cotton fiber development. To date, the molecular base GAs' functions development is largely unclear. address this question, endogenous bioactive GA levels developing fibers were elevated by specifically up-regulating 20-oxidase suppressing 2-oxidase via transgenic methods. Higher significantly increased micronaire values, 1000-fiber weight, cell wall thickness cellulose...
Cotton is the leading natural fiber crop in world. seeds are also an important oil and protein source. However, enhancement of abundance usually leads to a smaller seed. Thus, it has become challenge for cotton breeding concurrently increase yield seed yield. To improve yield, we elevated endogenous cytokinin level transgenic by constitutive suppression dehydrogenase (CKX), key negative regulator controlling plants. The slightly moderately suppressed plants showed normal growth development,...
Competition is one of the fundamental driving forces natural selection. Beauveria bassiana a soil and plant phylloplane/root fungus capable parasitizing insect hosts. Soil environments are often enriched with other fungi against which B. competes for survival. Here, we report an antifungal peptide (BbAFP1), specifically expressed localized to conidial cell wall released into surrounding microenvironment inhibiting growth competing fungi. strains expressing BbAFP1, including overexpression...
In plant immunity, the mutually antagonistic hormones salicylic acid (SA) and jasmonic (JA) are implicated in resistance to biotrophic necrotrophic pathogens, respectively. Promoters that can respond both SA JA signals urgently needed engineer plants with enhanced a broad spectrum of pathogens. However, few natural pathogen-inducible promoters available for this purpose. To address problem, we have developed strategy synthesize dual SA- JA-responsive by combining cis elements based on...
Cotton fibre is a single cell that derived from the ovule epidermis. Fibre initiation and elongation determine cotton yield quality. Sucrose most important substrate for cellulose synthesis energy production, as well provides turgor pressure to promote elongation. However, varieties with high-sugar content in fibres usually have short fibres, mechanism behind this unclear. Sugar transporter SUTs (sucrose transport proteins) SWEETs (Sugars Will Eventually be Exported Transporters), which are...
A balsam pear (Momordica charantia L.) chitinase (Mcchit1) was purified and sequenced at the N-terminal. The genomic cDNA coding sequences of Mcchit1 were cloned by rapid amplification 3′ ends (3′-RACE) Y-shaped adaptor dependent extension (YADE) method. Sequence analysis showed that protein is a class I containing chitin-binding domain catalytic domain, but no C-terminal extension. Northern blot indicated transcription wound-inducible. Overexpression dramatically increased intercellular...
Abstract Many toxic secondary metabolites produced by phytopathogens can subvert host immunity, and some of them are recognized as pathogenicity factors. Fusarium head blight Verticillium wilt destructive plant diseases worldwide. Using toxins the causal fungi graminearum dahliae screening agents, here we show that Arabidopsis P4 ATPases AtALA1 AtALA7 responsible for cellular detoxification mycotoxins. Through AtALA1-/AtALA7-mediated vesicle transport, sequestered in vacuoles degradation....
Abstract Cytokinin is considered to be an important driver of seed yield. To increase the yield cotton while avoiding negative consequences caused by constitutive overproduction cytokinin, we down-regulated specifically carpel genes for cytokinin oxidase/dehydrogenase (CKX), a key regulator levels, in transgenic cotton. The carpel-specific down-regulation CKXs significantly enhanced levels carpels. elevated promoted expression carpel- and ovule-development-associated genes, GhSTK2, GhAG1,...
Increasing the targeting ability of antifungal proteins towards specific components fungal cells has potential to improve their activity and reduce harmful effects nontarget cells. To obtain effective disease resistance genes against cotton Verticillium wilt, we constructed several fusion genes, in which binding domains chitin, sphingolipid or ergosterol cell wall membrane were individually fused peptide BbAFP1 from entomopathogenic fungus Beauveria bassiana. Transient expression cotyledons...
The entomopathogenic fungus Beauveria bassiana is a typical filamentous and has been used for pest biocontrol. Conidia are the main active agents of fungal pesticides; however, we know little about conidial developmental mechanisms less maturation mechanisms. We found that Zn2Cys6 transcription factor B. (named BbCmr1) was mainly expressed in late-stage conidia involved conidium regulation. Deletion Bbcmr1 impaired cell wall resulted lower germination rate under UV (UV), heat shock, H2O2,...
NmrA homologs have been reported as conserved regulators of the nitrogen metabolite repression (NMR) in various fungi. Here, we identified a homolog Verticillium dahliae and its functions utilization, growth development, pathogenesis. VdNmrA interacts with V. AreA protein regulates expression typical NCR target, formamidase gene. deletion mutants exhibited significantly slower colony on media Gln or Arg. Furthermore, impaired hyphal growth, spore production, hyperosmotic stress tolerance,...