A5035278677- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Microbial Metabolism and Applications
- Streptococcal Infections and Treatments
- Carbohydrate Chemistry and Synthesis
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Bacterial Genetics and Biotechnology
- Plant-Microbe Interactions and Immunity
- Fungal Biology and Applications
- Synthetic Organic Chemistry Methods
- Marine Sponges and Natural Products
- Bone Metabolism and Diseases
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
- Chemical Synthesis and Analysis
- Oral microbiology and periodontitis research
- Bone Tissue Engineering Materials
- Click Chemistry and Applications
- Biochemical and Structural Characterization
- Geothermal Energy Systems and Applications
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Bone and Dental Protein Studies
- Plant Disease Resistance and Genetics
Shanghai Institute of Organic Chemistry
2025
University of Chinese Academy of Sciences
2006-2025
Southwest University
2017-2024
Foshan Hospital of TCM
2021-2024
Chinese Academy of Sciences
2006-2023
Microbiology Institute of Shaanxi
2006-2023
Institute of Microbiology
2006-2023
Hebei University of Engineering
2022-2023
Henan Polytechnic University
2022
Weatherford College
2021
Several strategies have been used to clone large DNA fragments directly from bacterial genome. Most of these approaches are based on different site-specific recombination systems consisting a specialized recombinase and its target sites. In this study, novel strategy phage ϕBT1 integrase-mediated was developed, for simultaneous Streptomyces genome engineering cloning antibiotic gene clusters. This method has proved successful the actinorhodin cluster coelicolor M145, napsamycin daptomycin...
Two regulatory genes, jadR2 and jadR3, in the jadomycin (jad) biosynthetic gene cluster of Streptomyces venezuelae encode homologues γ-butyrolactone receptor. JadR2 was previously shown to be a pseudo jadR3 is situated at upstream jadW123 encoding putative enzymes for biosynthesis. Disruption resulted markedly decreased production jadomycin. Transcriptional analysis revealed that JadR3 represses jadW1, but activates jadR1, key activator DNase I footprinting showed has four binding sites...
In plant immunity, the mutually antagonistic hormones salicylic acid (SA) and jasmonic (JA) are implicated in resistance to biotrophic necrotrophic pathogens, respectively. Promoters that can respond both SA JA signals urgently needed engineer plants with enhanced a broad spectrum of pathogens. However, few natural pathogen-inducible promoters available for this purpose. To address problem, we have developed strategy synthesize dual SA- JA-responsive by combining cis elements based on...
Recently, we described the function of an uncharacterized two-gene regulatory system consisting a LytTR family transcription regulator and putative membrane protein, which referred to as hdrRM operon. In this study, determined that HdrRM controls expression analogous annotated SMU.2080 SMU.2081. Like hdrRM, SMU.2080-2081 operon encodes now refer BrsR BrsM respectively. Examination mechanism BrsRM suggests serves antagonize BrsR. Further analyses role it functions activator for variety...
The Streptococcus mutans hdrRM operon encodes a novel two-gene regulatory system induced by high cell density. Previous studies identified hdrM as the only known negative regulator of competence development in S. mutans. In present study, we demonstrated that HdrRM bypasses prototypical gene regulators ComC and ComDE transcriptional regulation competence-specific sigma factor comX late genes. Similarly, can abrogate requirement for ComE to produce bacteriocin mutacin IV. To further probe...
Previous studies have identified the hdrRM operon as a novel regulatory system induced by conditions of high cell density. Little is known about genes under control this system, but variety important phenotypes are associated with either hdrR overexpression or mutation hdrM. To characterize function HdrRM in Streptococcus mutans we used microarray approach to compare transcriptional profiles an strain hdrM mutant. Both strains exhibited almost identical profiles, which included all late...
Streptococcus mutans is a primary pathogen for dental caries in humans. CiaR and CiaH of S. comprise two-component signal transduction system (TCS) involved regulating various virulent factors. However, the that triggers CiaRH response remains unknown. In this study, we show calcium regulation ciaRH operon, double-glycine-containing small peptide encoded within operon (renamed ciaX) mediates regulation. CiaX contains serine + aspartate (SD) domain shared by calcium-binding proteins. A...
The aim of this study was to investigate Parkinson's disease (PD) using a murine model PD. Specifically, we aimed explore the mechanism by which puerarin prevents inflammation and apoptosis in neurocytes. Eighty healthy male C57/BL6 mice were randomly selected divided into four groups (N = 20 each): control group; PD PD+puerarin group. At end treatment period, animals' brains removed after perfusion decollation. protein expression levels tyrosine hydroxylase (TH) assessed...
Abstract Antimicrobial agents are urgently needed to tackle the growing threat of antibiotic-resistant pathogens. An important source new antimicrobials is large repertoire cryptic gene clusters embedded in microbial genomes. Genome mining revealed a napsamycin/mureidomycin biosynthetic cluster chromosome Streptomyces roseosporus NRRL 15998. The was activated by constitutive expression foreign activator ssaA from sansanmycin sp. strain SS. Expression verified RT-PCR analysis key genes....
Fusobacterium nucleatum is a gram-negative oral bacterial species associated with periodontal disease progression. This perhaps best known for its ability to adhere vast array of other bacteria and eukaryotic cells. Numerous studies F. have examined various coaggregation partners inhibitors, but it largely unknown whether these interactions induce particular genetic response. We tested between ATCC strain 25586 Streptococcus in the presence semidefined growth medium containing saliva. found...
Nikkomycins are competitive inhibitors of chitin synthase and inhibit the growth filamentous fungi, insects, acarids yeasts. The gene cluster responsible for biosynthesis nikkomycins has been cloned biosynthetic pathway was elucidated at genetic, enzymatic regulatory levels. Streptomyces ansochromogenes ΔsanL constructed by homologous recombination mutant strain fed with benzoic acid, 4-hydroxybenzoic nicotinic acid isonicotinic acid. Two novel nikkomycin analogues were produced when...