A5084868168- Biofuel production and bioconversion
- Plant Molecular Biology Research
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Plant nutrient uptake and metabolism
- Research in Cotton Cultivation
- Enzyme Catalysis and Immobilization
- Studies on Chitinases and Chitosanases
- Silk-based biomaterials and applications
- Plant biochemistry and biosynthesis
- Microbial bioremediation and biosurfactants
- Plant Gene Expression Analysis
- Biochemical and Structural Characterization
- Plant Pathogenic Bacteria Studies
- Pesticide and Herbicide Environmental Studies
- Bacteriophages and microbial interactions
- Plant Reproductive Biology
- Biopolymer Synthesis and Applications
- Polyamine Metabolism and Applications
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Plant Surface Properties and Treatments
- Genomics and Phylogenetic Studies
Tianjin Institute of Industrial Biotechnology
2014-2025
Chinese Academy of Sciences
2006-2025
Chongqing Academy of Agricultural Sciences
2017-2025
Ministry of Agriculture and Rural Affairs
2024-2025
Tianjin Economic-Technological Development Area
2023-2024
Washington University in St. Louis
2018-2022
Southwest University
2014-2022
University of Minnesota
2016-2018
Shanxi Normal University
2006-2016
Institute of Microbiology
2012-2015
Despite significant efforts to engineer their heterologous production, recombinant spider silk proteins (spidroins) have yet replicate the unparalleled combination of high strength and toughness exhibited by natural silks, preventing use in numerous mechanically demanding applications. To overcome this long-standing challenge, we developed a synthetic biology approach combining standardized DNA part assembly split intein-mediated ligation produce spidroins previously unobtainable size (556...
Bioactive gibberellins (GAs) comprise an important class of natural plant growth regulators and play essential roles in cotton fiber development. To date, the molecular base GAs' functions development is largely unclear. address this question, endogenous bioactive GA levels developing fibers were elevated by specifically up-regulating 20-oxidase suppressing 2-oxidase via transgenic methods. Higher significantly increased micronaire values, 1000-fiber weight, cell wall thickness cellulose...
ABSTRACT Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra burden and genetic instabilities. In this work, chromosomal was adapted the production mevalonate, which can be readily converted into β-methyl-δ-valerolactone, a monomer mechanically tunable polyesters. The mevalonate pathway, driven by constitutive promoter, integrated chromosome Escherichia coli to replace native gene adhE or...
Cotton is the leading natural fiber crop in world. seeds are also an important oil and protein source. However, enhancement of abundance usually leads to a smaller seed. Thus, it has become challenge for cotton breeding concurrently increase yield seed yield. To improve yield, we elevated endogenous cytokinin level transgenic by constitutive suppression dehydrogenase (CKX), key negative regulator controlling plants. The slightly moderately suppressed plants showed normal growth development,...
Auxin-dependent cell expansion is crucial for initiation of fiber cells in cotton (Gossypium hirsutum), which ultimately determines yield and quality. However, the regulation this process far from being well understood. In study, we demonstrate an antagonistic effect between cytokinin (CK) auxin on initiation. vitro planta experiments indicate that enhanced CK levels can reduce accumulation ovule integument, may account defects fiberless mutant xu142fl. turn, supplementation with recover...
Pullulan has many potential applications in the food, pharmaceutical, cosmetic and environmental industries. However, yield molecular properties of pullulan produced by various strains still need to be promoted fit application needs. A novel yeast-like strain Aureobasidium pullulans BL06 producing high weight (Mw) (3.3 × 106 Da) was isolated identified this study. The remarkable Mw A. highest level ever reported thus far. To further regulate biosynthesis BL06, three gene knockout ΔPMAs,...
Ogataea polymorpha, a non-conventional methylotrophic yeast, has demonstrated significant potential for heterologous protein expression and the production of high-value chemicals biopharmaceuticals. However, lack precise efficient genome editing tools severely hinders construction cell factories. Although CARISP-Cas9 system been established in gene efficiency, especially multiple genes edition, needs to be further improved. In this study, we developed an CRISPR-Cpf1-mediated O. polymorpha...
Methanol, which can come from methane or carbon dioxide, is a valuable renewable one-carbon (C1) feedstock for the production of biofuels and food chemicals. A new method was developed to create multienzyme complex by combining methanol dehydrogenase (Mdh), 3-hexulose-6-phosphate synthase (Hps), 6-phospho-3-hexuloseisomerase (Phi) in equal parts using SpyTag/Catcher DogTag/Catcher systems. This self-assembly multiple enzymes improves conversion fructose-6-phosphate (F6P) used engineer...
Fruit color is a crucial quality factor strongly influencing consumer preference for citrus. The coloration of citrus fruit primarily determined by carotenoids, which produce range hues. Gibberellic acid (GA) and ethylene are critical in during the ripening process. Nevertheless, underlying mechanisms remain poorly understood. present study utilized transcriptomic metabolomic analyses to investigate molecular regulatory affecting peel pigment metabolism tangors (Citrus reticulata...
To isolate and characterize a methyl parathion (MP)-mineralizing bacterium, to elucidate the degradative pathway of MP localize responsible degrading genes.A bacterial strain, designated B2, capable mineralizing was isolated from MP-polluted soil. Analysis 16S rRNA gene sequence phenotypic analysis suggested that strain B2 had close relationship with Ochrobactrum anthropi. could totally degrade four metabolites [p-nitrophenol (PNP), 4-nitrocatechol (4-NC), 1,2,4-benzenetriol (BT)...
Abstract Protein nanostructures with precisely defined geometries have many potential applications in catalysis, sensing, signal processing, and drug delivery. While de novo protein been assembled via non-covalent intramolecular intermolecular interactions, a largely unexplored strategy is to construct by covalently linking multiple individually folded proteins through site-specific ligations. Here, we report the synthesis of single-chain triangular square shapes made using copies...
Abstract Background The large family of pentatricopeptide repeat (PPR) proteins is widely distributed among land plants. Such play vital roles in intron splicing, RNA editing, processing, stability and translation. However, only a small number PPR genes have been identified rice. Results In this study, we raised mutant from tissue-culture-derived plants Oryza sativa subsp. japonica ‘Zhonghua 11’, which exhibited lethal chlorosis phenotype germination to the third-leaf stage. was designated...
Bacterial fatty acids (FAs) are an essential component of the cellular membrane and important source renewable chemicals as they can be converted to alcohols, esters, ketones, alkanes, used biofuels, detergents, lubricants, commodity chemicals. Most prior FA bioconversions have been performed on carboxylic acid group. Modification hydrocarbon chain could substantially expand structural functional diversity FA-derived products. Additionally, effects such modified FAs growth metabolic state...
Abstract Cytokinin is considered to be an important driver of seed yield. To increase the yield cotton while avoiding negative consequences caused by constitutive overproduction cytokinin, we down-regulated specifically carpel genes for cytokinin oxidase/dehydrogenase (CKX), a key regulator levels, in transgenic cotton. The carpel-specific down-regulation CKXs significantly enhanced levels carpels. elevated promoted expression carpel- and ovule-development-associated genes, GhSTK2, GhAG1,...
To understand the molecular basis of higher pH catalytic adaptation family 11 xylanases, we compared structures alkaline, neutral, and acidic active xylanases analyzed mutants xylanase Xyn11A-LC from alkalophilic Bacillus sp. SN5. It was revealed that alkaline have increased charged residue content, an ratio negatively to positively residues, decreased Ser, Thr, Tyr content relative non-alkaline counterparts. Between strands β6 β7, substitute α-helix for a coil or turn found in their...