A5101509573- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Lung Cancer Research Studies
- Lung Cancer Diagnosis and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA modifications and cancer
- Genetic factors in colorectal cancer
- Iterative Methods for Nonlinear Equations
- Medical Imaging and Pathology Studies
- Cancer Immunotherapy and Biomarkers
- Cancer-related Molecular Pathways
- Radiomics and Machine Learning in Medical Imaging
- Advanced Numerical Analysis Techniques
- Prostate Cancer Treatment and Research
- Advanced Numerical Methods in Computational Mathematics
- Virology and Viral Diseases
- Click Chemistry and Applications
- Biotin and Related Studies
- Entomopathogenic Microorganisms in Pest Control
- Histiocytic Disorders and Treatments
- Cancer, Lipids, and Metabolism
- AI in cancer detection
- Gene expression and cancer classification
- Intramuscular injections and effects
- Forecasting Techniques and Applications
Peking University
2015-2025
Peking University Cancer Hospital
2015-2025
Wenzhou Medical University
2024
Ruian People's Hospital
2024
Lanzhou University of Technology
2021
Southwest University
2020
Chinese Academy of Medical Sciences & Peking Union Medical College
2004-2019
Ministry of Education
2018
First Affiliated Hospital of Dalian Medical University
2015
Dalian Medical University
2015
Prostate cancer is significantly more common in Western men than Asian men, but the basis for this difference remains unknown. Because genomic studies of prostate are very limited, we used a genome-wide approach to reveal alterations Chinese cancers. We found significant reduction frequency certain somatic changes that commonly cancers, including 21q22.2-22.3 deletion, which involves TMPRSS2:ERG fusion gene, and 10q causes PTEN inactivation. Array results were confirmed by PCR-based...
Purpose Genetic intratumoral heterogeneity has a profound influence on the selection of clinical treatment strategies and addressing resistance to targeted therapy. The purpose this study was explore potential effect both genetic pathologic characteristics ALK-rearranged lung adenocarcinoma (LADC). Methods We tested ALK fusions EGFR mutations in 629 patients with LADC by using laser-capture microdissection capture spatially separated tumor cell subpopulations various subtypes test for...
Aims To compare fluorescence in situ hybridization (FISH), immunohistochemistry (IHC) and quantitative real-time reverse transcription-PCR (qRT-PCR) assays for detection of ROS1 fusion a large number ROS1-positive lung adenocatcinoma (ADC) patients. Methods Using IHC analysis, sixty ADCs including 16 cases with protein expression 44 without were selected this study. The status was examined by FISH qRT-PCR assay. Results Among 60 cases, (26.7%), 13 (21.7%) 20 (33.3%) positive revealed IHC,...
Competition is one of the fundamental driving forces natural selection. Beauveria bassiana a soil and plant phylloplane/root fungus capable parasitizing insect hosts. Soil environments are often enriched with other fungi against which B. competes for survival. Here, we report an antifungal peptide (BbAFP1), specifically expressed localized to conidial cell wall released into surrounding microenvironment inhibiting growth competing fungi. strains expressing BbAFP1, including overexpression...
Abstract There is a limited understanding of specific DNA methylation patterns associated with HER2 overexpression in breast and gastric cancers. Here we aim to solve the problem using inferred markers. data from The Cancer Genome Atlas (TCGA) were analyzed for cancers regarding status. We further applied targeted bisulfite sequencing approach elaborate profile region, covering 7635 CpG sites. Based on these two sets data, selected markers inferring status both validated their performance...
Background The study was conducted to investigate the clinicopathological features and prevalence of ROS1 gene fusion in Chinese patients with non‐small cell lung cancer (NSCLC). Methods presence assessed by quantitative real‐time PCR. Associations between clinical characteristics were analyzed. Results In total, 6066 pathologically confirmed NSCLC test results enrolled. average age 60.89 ± 10.60 years detected 157 (2.59%) patients. Fusion frequency significantly correlated age, gender,...
Compared with FISH and qRT-PCR analyses, immunohistochemistry (IHC) is the preferred screening test in most pathology practices for ALK-rearrangement detection. With 100% sensitivity 98% specificity, VENTANA ALK (D5F3) IHC assay has been approved EU some Asian countries However, an automated Ventana platform not available labs. In this study, we evaluated applicability of conventional D5F3 antibody routine pathological practice proposed detection methods procedures that ensure patients ALK+...
Background This study aimed to elucidate clinical significance of anaplastic lymphoma kinase (ALK) rearrangement in selected advanced non-small cell lung cancer (NSCLC), compare the application different ALK detection methods, and especially evaluate a possible association between expression outcomes crizotinib-treated patients. Methods status was assessed by fluorescent situ hybridization (FISH), immunohistochemistry (IHC) quantitative RT-PCR (qRT-PCR) 173 NSCLC Clinicopathologic data,...
Background: Lung adenocarcinoma (LUAD) possesses a poor prognosis with low 5-year survival rate even for stages I-III resected patients, it is thus critical to understand the determinants that affect and discover new potentially prognostic biomarkers. Somatic copy number alterations (CNAs) are major source of genomic variations driving tumor evolution, CNAs screening may identify Methods: Oncoscan MIP array was used analyze patterns on formalin fixed paraffin embedded(FFPE) specimens from...
In this study, we analyzed the genetic profiles of squamous cell lung carcinoma (SqCLC) to identify potential therapeutic targets. Approximately 2,800 COSMIC mutations from 50 genes were determined by next-generation sequencing. Amplification/deletion SOX2, CDKN2A, PTEN, FGFR1, EGFR, CCND1, HER2 and PDGFRA detected FISH expression VEGFR2, PD-L1 PTEN examined IHC. One hundred fifty-seven samples SqCLC collected. Somatic was identified in 73.9% cases, with TP53 (56.1%), CDKN2A (8.9%), PIK3CA...
// Di Ma 1, * , Zheng Wang 2, Lin Yang 3 Xinlin Mu 4 Yan 1 Xinming Zhao 5 Junling Li Dongmei 6 Department of Medical Oncology, Cancer Institute & Hospital, Chinese Academy Sciences, Beijing, China 2 Pathology, Beijing Hospital the Ministry Health, Respiratory and Critical Care Medicine, Peking University People's Diagnostic Radiology, Key Laboratory Carcinogenesis Translational Research (Ministry Education), Institute, These authors have contributed equally to this work Correspondence to:...
随着分子医学进展和靶向药物的不断涌现,晚期非小细胞肺癌(NSCLC)的治疗已进入到个体化治疗的时代.目前临床应用的个体化靶向治疗主要针对表皮生长因子受体(EGFR)基因突变性和间变性淋巴瘤激酶(ALK)融合基因阳性肺癌,这两种基因变异型肺癌均具有明确的分子靶点、靶点检测技术及上市的靶向药物,临床疗效得到明显提高。
The AURA3 clinical trial has shown that advanced non-small cell lung cancer (NSCLC) patients with EGFR T790M mutations in circulating tumor DNA (ctDNA) could benefit from osimertinib.The aim of this study was to assess the usefulness QuantStudio™ 3D Digital PCR System platform for detection plasma NSCLC patients, and compare performances ARMS-PCR.A total 119 Chinese were enrolled study. Mutant allele frequency detected by PCR, then 25 selected samples verified ARMS-PCR four them next...
The aim of the study was to characterize histological and epidemiological features lung cancer in Chinese women.
Objective To investigate the correlation and overlaps between PD-L1 expression classical genomic aberrations in Chinese lung adenocarcinoma (LADC) patients. Methods We reviewed 428 consecutive, surgically resected cases of LADC from October 2015 to December 2016 our center. was evaluated based on tumor proportion score (TPS). Correlation co-occurrence level with those driver genes, such as EGFR, ALK, ROS-1, KRAS clinical variables disease-free survival (DFS) were analyzed. Results Seventy...
Objective: This study aimed to investigate the prevalence of tumor programmed death-ligand 1 (PD-L1) expression in Chinese patients with advanced Non-Small Cell Lung Cancer (NSCLC). Methods: Tumor tissues histologically confirmed stage IIIB/IV NSCLC were retrospectively obtained from 10 centers China. PD-L1 was determined using IHC 22C3 pharmDx kit (Agilent, Santa Clara, CA, USA) and samples repetitively assayed Ab concentrate USA). Results: Out 901 who met inclusion criteria, 879 (97.6%)...
BACKGROUND: Elevated levels of tumor necrosis factor-alpha (TNF-α) have been associated with adverse pregnancy outcomes, specifically recurrent loss (RPL). These elevated may be the presence autoantibodies. Although TNF-α inhibitors shown promise in improving rates, further research is needed to comprehend their impact and mechanisms RPL patients. OBJECTIVE: This study aims investigate association between autoantibodies patients, as well evaluate effect inhibition on outcomes. METHODS: A...
Epidermal growth factor receptor (EGFR) is a novel target for therapy in subset of non-small cell lung cancer (NSCLC). Tumors with EGFR mutations showed good response to tyrosine kinase inhibitors (TKIs). We aimed identify the discriminating capacity immunohistochemistry (IHC) detect L858R and del E746-A750 NSCLC patients predict TKIs response.We collected specimens from 200 whose mutation status had been validated by direct DNA sequencing. IHC analyses using mutation-specific antibodies...