A5070955225- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Genital Health and Disease
- Prostate Cancer Diagnosis and Treatment
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Cervical Cancer and HPV Research
- Cancer-related gene regulation
- Urologic and reproductive health conditions
- Cancer Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Cancer-related Molecular Pathways
- Hormonal and reproductive studies
- Inflammatory Biomarkers in Disease Prognosis
- Urological Disorders and Treatments
- Metastasis and carcinoma case studies
- Cancer Genomics and Diagnostics
- Extracellular vesicles in disease
- Cancer and Skin Lesions
- RNA Research and Splicing
- Ethics and Legal Issues in Pediatric Healthcare
- Hepatocellular Carcinoma Treatment and Prognosis
- Oral Health Pathology and Treatment
- Nonmelanoma Skin Cancer Studies
- Protein Degradation and Inhibitors
Queen Mary University of London
2010-2023
Molecular Oncology (United States)
2023
Gdańsk Medical University
2023
Cancer Institute (WIA)
2012
Changhai Hospital
2010
Chinese Academy of Medical Sciences & Peking Union Medical College
2010
Second Military Medical University
2010
Optimum management of clinically localised prostate cancer presents unique challenges because the highly variable and often indolent natural history disease. To predict disease aggressiveness, clinicians combine clinical variables to create prognostic models, but models have limited accuracy. We assessed value a predefined cell cycle progression (CCP) score in two cohorts patients with cancer. measured expression 31 genes involved CCP quantitative RT-PCR on RNA extracted from formalin-fixed...
Prostate cancer is significantly more common in Western men than Asian men, but the basis for this difference remains unknown. Because genomic studies of prostate are very limited, we used a genome-wide approach to reveal alterations Chinese cancers. We found significant reduction frequency certain somatic changes that commonly cancers, including 21q22.2-22.3 deletion, which involves TMPRSS2:ERG fusion gene, and 10q causes PTEN inactivation. Array results were confirmed by PCR-based...
Castration-resistant prostate cancer (CRPC) is the major cause of death from cancer. Biomarkers to improve early detection and prediction CRPC especially using non-invasive liquid biopsies could outcomes. Therefore, we investigated plasma exosomal miRNAs associated with their potential for development into biomarkers resistance treatment. RNA-sequencing, which generated approximately five million reads per patient, was performed identify differentially expressed in 24 treatment-naive...
Abstract Fusion genes play important roles in tumorigenesis. The identification of the high-frequency TMPRSS2 fusion with ERG and other ETS family prostate cancer highlights importance solid tumor development progression. However, mechanisms leading to these fusions are unclear. We investigated whether androgen, through stimulating its receptor, could promote spatial genome reorganization contribute generation TMPRSS2:ERG fusion. show that treatment androgen can induce both malignant...
Background The pathogenesis of penile squamous cell carcinoma (PSCC) is not well understood, though risk factors include human papillomavirus (HPV). Disruption HER/PTEN/Akt pathway present in many cancers; however there little information on its function PSCC. We investigated HER family receptors and phosphatase tension homolog (PTEN) HPV-positive negative PSCC impact Akt activation using immunohistochemistry fluorescent situ hybridisation (FISH). Methodology/Principal Findings 148 PSCCs...
Abstract Many human cancers present as multifocal lesions. Understanding the clonal origin of is both etiological and clinical importance. The molecular basis prostate cancer has previously been explored using a limited number isolated markers and, although independent widely believed, still debatable. We attempted to address genome‐wide copy‐number analysis individual high‐grade prostatic intraepithelial neoplasia (HGPIN) Using Affymetrix array 6.0 analysis, we compared genomic changes...
Purpose: To develop an approach for the investigation of different subtypes circulating tumor cells (CTC) and other to evaluate their potential prognostic value prostate cancer.Experimental Design: Malignancy CTCs undergoing epithelial-to-mesenchymal transition (EMT) was confirmed by repeated FISH. Subgroups in 81 patients with cancer (43 castration resistant 38 untreated localized) were correlated disease aggressiveness parameters. AUC analysis applied compare performance metastasis...
Background Docetaxel improves overall survival (OS) in castration-resistant prostate cancer (PCa) (CRPC) and metastatic hormone-sensitive PCa (mHSPC). However, not all patients respond due to inherent and/or acquired resistance. There remains an unmet clinical need for a robust predictive test stratify treatment. Liquid biopsy of circulating tumour cell (CTCs) is minimally invasive, can provide real-time information the heterogeneous therefore may be potentially ideal docetaxel response...
Abstract We recently found that TMPRSS2:ERG fusion genes and PTEN loss, which are common in Western prostate cancers infrequent Chinese cases. As previous studies indicated a higher frequency of RAS BRAF mutation rates Eastern Asian than involving the RAF family RAF1 were identified cancer American population, we investigated alterations cancer. Using fluorescence situ hybridization, was truncated five 200 informative cases (2.5%) three 204 (1.5%) genomic rearrangements these significantly...
Aims To determine whether Ki-67 immunoexpression in penile squamous cell carcinoma (PSCC) has a prognostic value and correlates with lymph node metastasis, human papillomavirus (HPV) infection patient survival. Methods 148 formalin-fixed paraffin-embedded PSCC samples were tissue-microarrayed, including 97 usual-type SCCs, 17 basaloid, 15 pure verrucous carcinomas, 2 warty mixed-type tumours. All immunostained for protein. HPV DNA was detected INNO-LiPA assay. Follow-up data available 134...
Prostate specific antigen testing results in unnecessary biopsy and over diagnosis with consequent overtreatment. Tissue is an invasive procedure associated significant morbidity. More accurate noninvasive or minimally diagnostic approaches should be developed to avoid prostate diagnosis. We investigated the potential of using circulating tumor cell analysis cancer diagnosis, particularly predict clinically prebiopsy cases.We enrolled 155 treatment naïve patients 98 before for enumeration....
Stankiewicz E, Prowse D M, Ktori Cuzick J, Ambroisine L, Zhang X, Kudahetti S, Watkin N, Corbishley C & Berney M (2011) Histopathology 58 , 433–439 The retinoblastoma protein/p16 INK4A pathway but not p53 is disrupted by human papillomavirus in penile squamous cell carcinoma Aims: pathogenesis of (PSCC) well understood. Human (HPV) may be involved carcinogenesis, few studies have compared cell‐cycle protein expression HPV positive and negative cancers. aim was to determine the extent...
Penile squamous cell carcinoma is a rare disease, in which somatic genetic aberrations have yet to be characterized. We hypothesized that gene copy might correlate with human papillomavirus status and clinico-pathological features. sought determine the spectrum of number large series PSCCs define their correlations papillomavirus, histopathological subtype, tumor grade, stage lymph node status. Seventy formalin-fixed, paraffin embedded penile carcinomas were centrally reviewed by expert...
Abstract Prostate cancer is the most common among western men, with a significant mortality and morbidity reported for advanced metastatic disease. Current understanding of disease limited due to difficulty sampling as prostate mainly metastasizes bone. By analysing bone metastases using high density microarrays, we found genomic copy number loss at 6q16.1–16.2, containing FBXL4 gene, which was confirmed in larger series by fluorescence situ hybridisation (FISH). Loss also detected primary...
To determine whether phosphatidylinositol-4,5-bisphosphate 3- kinase, catalytic subunit alpha (PIK3CA) copy number gain is common and could prove a useful marker for the activation status of PI3K-AKT-mTOR pathway in penile squamous cell carcinoma (PSCC).Fresh frozen tissue archival blocks were collected from 24 PSCC patients with 15 matched normal epithelium (NPE) St George's Hospital. PIK3CA mutational (CNS) was assessed via Sanger sequencing fluorescence in-situ hybridisation,...
Next-generation sequencing of primary tumors is now standard for transcriptomic studies, but microarray-based data still constitute the majority available information on other clinically valuable samples, including archive material. Using prostate cancer (PC) as a model, we developed robust analytical framework to integrate across different technical platforms and disease subtypes connect distinct stages reveal potentially relevant genes not identifiable from single studies alone. We...
// Xueying Mao 1, * , Fei Luo 2, 3, Lara K. Boyd Bowei Zhou 3 Yanling Zhang 4, 5 Elzbieta Stankiewicz 1 Jacek Marzec Natasa Vasiljevic 6 Yongwei Yu 7 Ninghan Feng 8 Jia Xu Attila Lorincz Yong Jiang Claude Chelala Guoping Ren 4 Daniel M Berney Shan-Chao Zhao 2 Yong-Jie Lu Centre for Molecular Oncology, Barts Cancer Institute, and the London School of Medicine Dentistry, Queen Mary University London, EC1M 6BQ, UK Department Urology, Nanfang Hospital, Southern Medical University, Guangzhou,...
Background Therapeutic targeting of the PI3K-AKT-mTOR pathway may benefit patients with advanced penile squamous cell carcinoma (PSCC). Objectives To determine prevalence PIK3CA copy number gain and correlate this activity status in pre-malignant intraepithelial neoplasia (PeIN) invasive PSCC. Materials methods Archival tissue blocks were obtained from 58 PeIN 244 primary PSCC treated at St George’s Hospital. (CNS) was assessed by fluorescence in-situ hybridisation. High-risk HPV DNA...
To examine the tissue expression of DNA topoisomerase I (Topo I) and IIalpha II), to pursue possibility future chemotherapy regimens for squamous cell carcinoma penis (SCCP), as high Topo might indicate sensitivity camptothecins, whereas II etoposide.In all, 73 patients with SCCP were reviewed then samples microarrayed. These stained immunohistochemistry I, Ki-67. Tumour stage, grade type available.Topo showed a strong positive correlation proliferation index measured by Ki-67 (P < 0.001)...