A5101745343- Pancreatitis Pathology and Treatment
- Endoplasmic Reticulum Stress and Disease
- Advanced Glycation End Products research
- Pancreatic and Hepatic Oncology Research
- Phagocytosis and Immune Regulation
- Animal Virus Infections Studies
- Inflammasome and immune disorders
- S100 Proteins and Annexins
- Heme Oxygenase-1 and Carbon Monoxide
- Virus-based gene therapy research
- Antibiotic Resistance in Bacteria
- RNA Research and Splicing
- Viral Infections and Vectors
- Parvovirus B19 Infection Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Vector-borne infectious diseases
- Peroxisome Proliferator-Activated Receptors
- Mosquito-borne diseases and control
- Diabetic Foot Ulcer Assessment and Management
- Respiratory Support and Mechanisms
- Acute Ischemic Stroke Management
- Prostate Cancer Treatment and Research
- Kawasaki Disease and Coronary Complications
- Inflammatory mediators and NSAID effects
- Nitric Oxide and Endothelin Effects
Southern Medical University
2006-2022
Chengdu Medical College
2020
University of Pittsburgh Medical Center
2015
University of Pittsburgh
2011-2014
Hemorrhagic shock (HS) due to major trauma and surgery predisposes the host development of systemic inflammatory response syndrome (SIRS), including acute lung injury (ALI), through activating exaggerating innate immune response. IL-1β is a crucial proinflammatory cytokine that contributes SIRS ALI. Lung endothelial cells (EC) are one important source IL-1β, production active controlled by inflammasome. In this study, we addressed mechanism underlying HS activation inflammasome in EC. We...
Abstract Chronic pancreatitis (CP) is a progressive, recurrent inflammatory disorder of the pancreas. Initiation and progression CP can result from serine protease 1 (PRSS1) overaccumulation ensuing endoplasmic reticulum (ER) stress. However, how ER stress pathways regulate development remains poorly understood. In present study we aimed to elucidate pathway involved in CP. We found high expression marker genes ATF6, XBP1, CHOP human clinical specimens. A humanized PRSS1 transgenic mouse was...
In patients with peripheral artery disease, blockages in arterioles <1 mm cannot be treated surgically, and there are currently few effective medicines. Studies have shown that inflammation ischemic tissue is related to injury recovery angiogenesis, but insufficient attention has been paid this area. suggested HMGB1 (high mobility group protein 1), which released by tissue, promotes the mechanism not entirely clear. study, we tested internalization of endothelial cells investigated a novel...
Toll-like receptors (TLRs) play an important role in regulating muscle regeneration and angiogenesis response to ischemia. TLR2 knockout mice exhibit pronounced skeletal necrosis abnormal vessel architecture after femoral artery ligation, suggesting that signaling is protective during TLR4, receptor inflammatory signaling, has been shown regulate expression other systems. We hypothesize a similar relationship between TLR4 may exist hindlimb ischemia which upregulates TLR2, mediator of...
Background: Pancreatic cancer (PC) is prevalent among malignant tumors with poor prognosis and lacks efficient therapeutic strategies. Endoplasmic reticulum (ER) stress apoptosis are associated chronic inflammation progression. However, the prognostic value of ER stress-related, apoptosis-related genes in PC remains to be further elucidated. Our study aimed at confirming values stress-related genes, ATF6, EMC6, XBP1, CHOP, gene, APAF1, patients. Methods: Gene Expression Profiling Interactive...
Abstract Treatment of acute pancreatitis (AP) and chronic (CP) remains problematic due to a lack knowledge about disease-specific regulatory targets mechanisms. The purpose this study was screen proteins related endoplasmic reticulum (ER) stress apoptosis pathways that may play role in pancreatitis. Human pancreatic tissues including AP, CP, healthy volunteers were collected during surgery. Humanized PRSS1 (protease serine 1) transgenic ( Tg ) mice constructed treated with caerulein mimic...
Abstract Myeloid‐related protein 8/14 (MRP8/14) participates in various inflammatory responses, however, its effect on macrophage efferocytosis remains unclear. Here, we demonstrate that MRP8/14 significantly inhibits the of apoptotic thymocytes by mouse bone marrow‐derived macrophages (BMDMs), which later proves to be associated with receptor for advanced glycation end products (RAGE) or reducing expression growth arrest‐specific 6 and milk fat globule epidermal factor 8, independent RAGE....
Abstract Background Early diagnosis and treatment of chronic pancreatitis (CP) are limited. In this study, St13, a co-chaperone protein, was investigated whether it constituted novel regulatory target in CP. Meanwhile, we evaluated the value micro-PET/CT early Methods Data from healthy control individuals patients with alcoholic CP (ACP) or non-ACP (nACP) were analysed. PRSS1 transgenic mice ( Tg ) treated ethanol caerulein to mimic development ACP nACP, respectively. Pancreatic lipid...
// Xueying Mao 1, * , Fei Luo 2, 3, Lara K. Boyd Bowei Zhou 3 Yanling Zhang 4, 5 Elzbieta Stankiewicz 1 Jacek Marzec Natasa Vasiljevic 6 Yongwei Yu 7 Ninghan Feng 8 Jia Xu Attila Lorincz Yong Jiang Claude Chelala Guoping Ren 4 Daniel M Berney Shan-Chao Zhao 2 Yong-Jie Lu Centre for Molecular Oncology, Barts Cancer Institute, and the London School of Medicine Dentistry, Queen Mary University London, EC1M 6BQ, UK Department Urology, Nanfang Hospital, Southern Medical University, Guangzhou,...
Inducible nitric oxide synthase (iNOS) plays a central role in tissue damage during endotoxic shock. However, the underlying mechanisms that control transcription of iNOS are not completely defined. A screening strategy with DNA pull-down technology and two-dimensional difference gel electrophorcsis (2D-DIGE) analysis was used to identify regulators interact promoter. We found 14 proteins bind promoter liver shock mice. After matrix-assisted laser desorption/ionization time flight mass...
Background: Acute pancreatitis is a severe pancreatic disorder that remains associated with high mortality due to lack of effective drugs and management strategies. This study aimed investigate the molecular pathogenic mechanisms acute involving p53 endoplasmic reticulum (ER) stress pathways.Methods: Expression PRSS1 in human tissues was detected by immunohistochemistry western blotting. induced caerulein humanized transgenic mice, its severity verified histological imaging, evaluation...
Objective To construct the vector of mouse HMGB1 promoter-driven red fluorescent protein reporter gene so as to supply a tool for study on expression regulation in mammalian cells and related signal transduction mechanism. Methods The promoter sequence was subcloned into vector, pDsRed1-1. recombinant pDsRed1-1-HMGB1p then transfected NIH3T3 by liposome, intracellular activity observed under fluorescence microscope normal condition or after tumor necrosis factor-α (TNF-α) stimulation....
Abstract Acinetobacter baumannii is an emergency pathogenic bacterium for its multidrug-resistance and high mortality rates after infection. In-depth genetic analysis of A. virulence drug-resistant genes highly desirable. Existing methods manipulation mainly rely on the use antibiotic as selectable marker, sacB /sucrose counter-selectable which inconvenient inappropriate all research . Based conserved pyrF gene 500bp-flanking sequence, we quickly easily generated -deleted mutants uracil...
Background: Treatment of acute pancreatitis (AP) and chronic (CP) remains problematic due to a lack knowledge about disease-specific regulatory targets mechanisms. The purpose this study was screen proteins related endoplasmic reticulum (ER) stress apoptosis pathways that may play role in pancreatitis.Methods: Human pancreatic tissues including AP, CP healthy volunteers were collected during surgery. Humanized PRSS1 transgenic mice constructed treated with caerulein mimic the development...