A5101770899- Endoplasmic Reticulum Stress and Disease
- Sphingolipid Metabolism and Signaling
- Liver Disease Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Pancreatic and Hepatic Oncology Research
- Microtubule and mitosis dynamics
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer, Lipids, and Metabolism
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Ion Channels and Receptors
- Liver Disease and Transplantation
- Drug Transport and Resistance Mechanisms
- T-cell and B-cell Immunology
- Liver physiology and pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pituitary Gland Disorders and Treatments
- Viral Infections and Vectors
- RNA modifications and cancer
- Lysosomal Storage Disorders Research
- Diet, Metabolism, and Disease
- Biochemical effects in animals
- Pancreatitis Pathology and Treatment
- Tryptophan and brain disorders
Southern Medical University
2016-2025
Nanfang Hospital
2015-2025
Sun Yat-sen University
2019
Sun Yat-sen University Cancer Center
2019
Columbia University
2015-2018
Guangzhou Medical University
2015
Shenzhen Maternity and Child Healthcare Hospital
2012
Kobe University Hospital
2002
Abstract Aims Nuclear protein 1 (Nupr1) is a multifunctional stress-induced involved in the regulation of tumorigenesis, apoptosis, and autophagy. However, its role pulmonary hypertension (PH) after METH exposure remains unexplored. In this study, we aimed to investigate whether can induce PH describe mechanism Nupr1 development PH. Methods results Mice were made upon chronic intermittent treatment with METH. Their right ventricular systolic pressure (RVSP) was measured assess artery...
Dyshomeostasis of both ceramides and sphingosine-1-phosphate (S1P) in the brain has been implicated aging-associated neurodegenerative disorders humans. However, mechanisms that maintain homeostasis these bioactive sphingolipids remain unclear. Mouse alkaline ceramidase 3 (Acer3), which preferentially catalyzes hydrolysis C18:1-ceramide, a major unsaturated long-chain ceramide species brain, is upregulated with age mouse brain. Acer3 knockout causes an age-dependent accumulation various...
Hepatocellular carcinoma (HCC) has been extensively studied as one of the most aggressive tumors worldwide. However, its mortality rate remains high due to ideal diagnosis and treatment strategies. Uncovering novel genes with prognostic significance would shed light on improving HCC patient's outcome. In our study, we applied data-independent acquisition (DIA) quantitative proteomics investigate expression landscape 24 paired patients. A total 1029 differentially expressed proteins (DEPs)...
Many viruses, including adenovirus, exhibit bidirectional transport along microtubules following cell entry. Cytoplasmic dynein is responsible for microtubule minus end of adenovirus capsids after endosomal escape. However, the identity and roles opposing plus end-directed motor(s) remain unknown. We performed an RNAi screen 38 kinesins, which implicated Kif5B (kinesin-1 family) additional minor kinesins in 5 (Ad5) capsid translocation. markedly increased centrosome accumulation incoming Ad5...
Abstract Post-hepatectomy liver dysfunction is a life-threatening morbidity that lacks efficient therapy. Bioactive lipids involved in macrophage polarization crucially regulate tissue injury and regeneration. Herein, we investigate the key bioactive mediate cytotherapeutic potential of polarized-macrophage for post-hepatectomy dysfunction. Untargeted lipidomics identified elevation ceramide (CER) metabolites as signature lipid species relevant to M1/M2 mouse bone-marrow-derived-macrophages...
Abstract Bile acid overload critically drives the pathogenesis of cholestatic liver injury (CLI). While ceramide metabolism has garnered increasing interest in research, role ceramides CLI remains unclear. This study investigates function alkaline ceramidase 3 (ACER3)-catalyzed hydrolysis unsaturated CLI. Using clinical specimens, this work finds that ACER3 expression is upregulated and positively correlated with severity patients. Acer3 ablation increases ceramide(d18:1/18:1) attenuates...
Overload of palmitic acids is linked to the dysregulation ceramide metabolism in nonalcoholic steatohepatitis (NASH), and ceramides are important bioactive lipids mediating lipotoxicity acid NASH. However, much remains unclear about role ceramidases that catalyze hydrolysis By analyzing National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database, we found alkaline ceramidase 3 (ACER3) upregulated livers patients with Consistently, Acer3 mRNA levels its...
The machinery that prevents colorectal cancer liver metastasis (CRLM) in the context of regeneration (LR) remains elusive. Ceramide (CER) is a potent anti-cancer lipid involved intercellular interaction. Here, we investigated role CER metabolism mediating interaction between hepatocytes and metastatic (CRC) cells to regulate CRLM LR.Mice were intrasplenically injected with CRC cells. LR was induced by 2/3 partial hepatectomy (PH) mimic LR. alteration corresponding CER-metabolizing genes...
BACKGROUND Liver failure after resection for liver cancer is associated with increased patient mortality. This study aimed to investigate the mechanism of protective effects resveratrol, a natural plant-derived compound, on injury in rat model partial hepatectomy. MATERIAL AND METHODS Adult male Sprague-Dawley (SD) rats (n=60) were divided into sham group (n=20), and plus resveratrol-treated (n=20). removed 2/3 resection; resveratrol was given at dose 30 mg/kg/day from one week before...
Abstract IL-27 is a heterodimeric cytokine that composed of two subunits, i.e., EBV-induced gene 3 and IL-27p28 (also known as IL-30). Although the role endogenous in pathogenesis autoimmune colitis, an experimental model human inflammatory bowel disease, remains controversial, local delivery has been shown to inhibit colitis. IL-30 Th1 Th17 responses considered potential therapeutic for certain diseases. In this study, we have compared efficacy adeno-associated viral vector-delivered murine...
The aim of the present study was to identify differentially expressed genes (DEGs) between primary tumor tissue and adjacent non‑tumor hepatocellular carcinoma (HCC) samples in order investigate mechanisms HCC. microarray data datasets GSE76427, GSE84005 GSE57957 were downloaded from Gene Expression Omnibus database. DEGs identified using limma package R programming language. Following intersection screened three datasets, 218 selected for further study. A protein‑protein interaction (PPI)...
Activation of Kupffer cell (KC) is acknowledged as a key event in the initiation and perpetuation bile duct warm ischemia/reperfusion injury. The inhibitory effect gadolinium chloride (GdCl(3)) on KC activation shows potential protective intervention liver injury, but there less research with regard to injury.Sixty-five male Sprague-Dawley rats (200-250 g) were randomly divided into three experimental groups: sham group (n = 15), control 25), GdCl(3) 25). Specimen was collected at 0.5, 2, 6,...
Multi-drug resistance to chemotherapeutic agents is a major cause of treatment failure in breast cancer. In this study, we investigated the effects emodin on reversing multi-drug resistance, examined ERCC1 protein expression cancer cell line, and explored relationship between reversal expression. MTT assay was conducted test cytotoxicity adriamycin cisplatin MCF-7/Adr cells with without pretreatment, Western blot performed examine had 21-fold 11-fold baseline resistances cisplatin,...
Tau is a member of microtubule-associated proteins (MAPs) and expressed in normal breast epithelium cancer cells. expression levels early were correlated with the responsiveness taxane-containing chemotherapy. However, it unknown whether contributes to progression. Herein, recurrent metastatic (RMBC) its predictive significance palliative chemotherapy investigated. Immunohistochemical (IHC) staining was conducted detect protein biopsies from 285 patients RMBC, correlation between sensitivity...
Patient selection is critically important in improving the outcomes of liver transplantation for hepatocellular carcinoma. The aim current study was to identify biochemical measures that could affect patient prognosis after transplantation.A total 119 patients receiving carcinoma were used construct a model predicting recurrence. results validated using an independent sample 109 from hospitals. All subjects both cohorts met Hangzhou criteria.Analysis discovery cohort revealed association...
Methamphetamine (METH) chronic exposure is an important risk factor for hypertension development. However, the mechanisms behind METH-induced remain unclear. Therefore, we aimed to reveal potential underlying hypertension.
Stem cell antigen-1 (Sca-1/Ly6A/E) is a surface glycoprotein that often used as biomarker for stem cells and stemness. However, it not clear what factors can directly induce the expression of Sca-1/Ly6A/E in T lymphocytes vivo, if induction Sca-1 associated with In this study, we show interleukin-27 (IL-27), member IL-12 family cytokines, induces vivo. We found mice-deficient IL-27 (either P28 or EBI3) its signalling (IL-27Rα) had profound reduction naive (CD62L+ CD44- ), memory CD44+ )...