A5026564034- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Respiratory viral infections research
- Hepatitis Viruses Studies and Epidemiology
- HIV/AIDS Research and Interventions
- Animal Disease Management and Epidemiology
- Adolescent Sexual and Reproductive Health
- HIV, Drug Use, Sexual Risk
- Pediatric health and respiratory diseases
- Water Quality Monitoring and Analysis
- Analytical Methods in Pharmaceuticals
- Demographic Trends and Gender Preferences
- Analytical Chemistry and Chromatography
- Hepatitis B Virus Studies
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Advanced Thermodynamic Systems and Engines
- HIV/AIDS Impact and Responses
- Sex work and related issues
- Pharmacological Effects and Assays
- CRISPR and Genetic Engineering
- COVID-19 and healthcare impacts
- T-cell and Retrovirus Studies
Southern Medical University
2012-2024
Cincinnati Children's Hospital Medical Center
2012-2015
Hunan Normal University
2010
Hunan Provincial People's Hospital
2010
Nanfang Hospital
2004
Abstract During the past norovirus (NoV) epidemic season, a new GII.17 variant emerged as predominant NoV strain, surpassed GII.4 NoVs, causing outbreaks of acute gastroenteritis (AGE) in China. Here we report study an AGE outbreak elementary school December 2014 caused by to explore potential mechanism behind sudden epidemics NoV. A total 276 individuals were sick with typical infection symptoms vomiting (93.4%), abdominal pain (90.4%), nausea (60.0%) and diarrhea (10.4%) at attack rate...
Hand, foot, and mouth disease (HFMD) is caused by human enteroviruses, especially enterovirus 71 (EV71) coxsackievirus A16 (CA16). Patients infected with different enteroviruses show varied clinical symptoms. The aim of this study was to determine whether the etiological spectrum mild severe HFMD changed, association between pathogens features. From 2009 2013, a total 2,299 stool or rectal specimens were collected corresponding patient data. A dynamic view in Shenzhen city China provided....
Noroviruses (NoVs) are the most important viral pathogens that cause epidemic acute gastroenteritis. NoVs recognize human histo-blood group antigens (HBGAs) as receptors or attachment factors. The elucidation of crystal structures HBGA-binding interfaces a number representing different HBGA binding patterns opens new strategy for development antiviral compounds against through rational drug design and computer-aided virtual screening methods. In this study, docking simulations were used to...
Abstract Rotaviruses are known to recognize human histo-blood group antigens (HBGAs) as a host ligand that is believed play an important role in rotavirus susceptibility and range. In this study, paired fecal saliva samples collected from children with viral gastroenteritis, well serum the general population south China were studied evaluate potential association between infections HBGA phenotypes. Rotavirus was detected 75 (28%) of 266 P[8] rotaviruses found be predominant genotype. The...
Introduction Norovirus (NoV) is one of the most important agents responsible for viral acute gastroenteritis, among which GII.4 NoV predominant strain worldwide, and GII.17 surpassed in some epidemic seasons. Rapid accurate gene recognition essential a timely response to outbreaks. Methods In present study, highly conserved regions NoVs were identified junction open reading frame (ORF) 1 ORF2 then amplified by isothermal recombinase-aided amplification (RAA), followed cleavage CRISPR-Cas13a...
A novel GII.17 norovirus variant caused major gastroenteritis epidemics in China 2014 to 2016. To explore the host immune factors selection of emergence this new variant, we characterized its antigenic relatedness with GII.4 noroviruses that have dominated for decades. Through an enzyme-linked immunosorbent assay (ELISA) and a histo-blood group antigen (HBGA) blocking using sera from variant-infected patients, respectively, observed limited cross-immune reactivity by ELISA but little HBGA...
The GII.4 noroviruses (NoVs) are a single genotype that is responsible for over 50% of NoV gastroenteritis epidemics worldwide. However, NoVs have been found to undergo antigenic drifts, likely selected by host herd immunity, which raises an issue vaccine strategies against NoVs. We previously characterized variations and significant levels relatedness among different variants. Further characterization the genetic recent variants (2008b 2010 cluster) was performed in this study. amino acid...
Abstract An acute gastroenteritis (AGE) outbreak caused by a norovirus occurred at hospital in Shanghai, China, was studied for molecular epidemiology, host susceptibility and serological roles. Rectal environmental swabs, paired serum samples saliva specimens were collected. Pathogens detected real-time polymerase chain reaction DNA sequencing. Histo-blood group antigens (HBGA) phenotypes of their binding to protruding proteins determined enzyme-linked immunosorbent assay. The HBGA-binding...
Noroviruses (NoVs) are an important cause of acute gastroenteritis but knowledge on the disease burden and epidemiology in children developing countries remains limited. In this study, we performed a surveillance NoV China to address some questions.Faecal specimens from (<5 years age) at outpatient clinics Nan Fang Hospital Guangzhou, during fall-winter seasons 2003-2006 were tested for rotaviruses (RVs) NoVs. A questionnaire clinical records hygiene habits was collected each patient.Among...
An HPLC-MS/MS method has been developed and validated for the determination of venlafaxine enantiomers in human plasma applied to a pharmacokinetic study healthy Chinese volunteers. The was carried out on vancomycin chiral column (5 µm, 250 × 4.6 mm) maintained at 25°C. mobile phase methanol-water containing 30 mmol/L ammonium acetate, pH 3.3 adjusted with aqueous ammonia (8:92, v/v) flow rate 1.0 mL/min. A tandem mass spectrometer an electrospray interface operated multiple reaction...
ABSTRACT Noroviruses (NVs) are important causes of nonbacterial gastroenteritis in humans, but the role NVs as a cause diseases Chinese people, particularly military personnel, remains unclear. This study investigated antibody prevalence and factors that associate with to among students attending medical university. Serum specimens were tested by an enzyme-linked immunosorbent assay for immunoglobulin G recombinant capsid antigens three (rNorwalk, rMxV, rVA387). Of 588 serum samples tested,...
Abstract Human norovirus is a leading cause of foodborne illness worldwide, and its detection in foods remains challenge due to the difficulty eluting enriching trace amounts viral particles. In this study, we developed porcine gastric mucin–labeled magnetic capture probes optimized conditions for their binding with norovirus, elution particles, RNA isolation based on previous research. We evaluated capture‐based pretreatment method artificially contaminated strawberry samples compared it...
The prevalence of HIV/HCV/HBV/ Treponema pallidum is an essential health issue in China. However, there are few studies focused on foreigners living This study aimed to assess the and socio-demographic distribution HIV, HBV, HCV, T. among Guangzhou period 2010-2017.A cross-sectional was conducted screen serological samples 40,935 from 2010 2017 at Guangdong International Travel Health Care Center Guangzhou. Samples were tested for hepatitis B surface antigen (HBsAg), anti-HCV, syphilis...
High under-five mortality rate remains one of the public health challenges, especially in Sub-Saharan Africa, accounting for more than half all global cases. Sierra Leone was and is still countries with highest rate. Using latest 2019 Demographic Health Survey data, we investigated factors associated Leone. A total 9771 mothers aged 15-49 years country were interviewed included analysis. The dependent variable child status (dead = 1; alive 0). 871 (9%) children died before their fifth...
Noroviruses (NoVs) are the leading viral pathogens globally causing acute gastroenteritis (AGE) in humans, posing a significant global health threat and economic burden. Recent investigations revealed that human NoVs had been detected different animals, which raises concerns about whether potential zoonotic diseases. This study developed novel luciferase immunosorbent assay (LISA) to detect GII.6 NoV IgG based on P protein of VP1. The LISA showed high specificity (99.20%) sensitivity...
Characterizing diversity and the antigenic relatedness of norovirus remains a primary focus in understanding its biological properties vaccine designs. The precise serological features GI genotypes have not been studied. study represented an investigation on gastroenteritis outbreak related to GI.3 three most detected genotypes, GI.2 (belonging immunotype B), GI.9 C), were selected characterize their phylogenetic relationship, HBGA binding profiles within (intra-immunotype), between...