Free proline has multiple functions in plant cells, such as regulating osmotic potential and protecting both proteins cell membranes. The expression of Δ1-Pyrroline-5-carboxylate synthase (P5CS), a key enzyme the biosynthetic pathway, increases under drought, salt cold stress conditions, causing cells to accumulate large amounts proline. In this study, we cloned identified P5CS gene from Stipa purpurea, which full-length 2196 bp encodes 731 amino acids. A subcellular localization analysis...

Abstract Hippophae gyantsensis , which is a native tree species in China, ideal for windbreak and sand-fixing forests. It an economically ecologically valuable distributed exclusively the Qinghai-Tibet Plateau China. In our study, we assembled chromosome-level genome of H. using Illumina sequencing, Nanopore sequencing chromosome structure capture technique. The was 716.32 Mb size with scaffold N50 length 64.84 Mb. A total 716.25 data anchored orientated onto 12 chromosomes mounting rate up...

Dithioacetals are a frequently used motif in synthetic organic chemistry, and most existing reports discuss only symmetrical dithioacetals. Examples of unsymmetrical dithioacetals scarce, few general methods for the selective synthesis these compounds exists. An intriguing visible-light-induced strategy has been established this work sequential reactions S–H insertion acetal exchange between acylsilanes two different thiols that deliver wide variety moderate yields. The were obtained with...

3D Referring Expression Segmentation (3D-RES) aims to segment point cloud scenes based on a given expression. However, existing 3D-RES approaches face two major challenges: feature ambiguity and intent ambiguity. Feature arises from information loss or distortion during acquisition due limitations such as lighting viewpoint. Intent refers the model's equal treatment of all queries decoding process, lacking top-down task-specific guidance. In this paper, we introduce an Image enhanced Prompt...

Abstract Lysine acetylation is pivotal in regulating growth, development, and stress responses across numerous plant species. This modification, mediated by lysine acetyltransferases (KATs) deacetylases (KDACs), both dynamic reversible. Despite the economic significance of grape ( Vitis vinifera ) as a fruit crop, comprehensive insights into its KAT KDAC gene families remain limited. In this study, 8 VvKATs 14 VvKDACs were identified within genome. Detailed analyses their physicochemical...

Drip irrigation (DI) has been widely utilized for crops and its water-saving effect confirmed by numerous studies. However, whether this technology can save so much water under the field scale during practical application is still uncertain. In order to answer question, evapotranspiration (ET), soil content, transpiration evaporation over DI border (BI) in an arid area of NW China were continuously measured two eddy covariance systems, micro-lysimeters, packaged stem sap flow gauges CS616...

Abstract Background Under the escalating threat to sustainable development from global increase in carbon dioxide concentrations, variations flux farmland ecosystem and their influencing factors have attracted attention. Over past few decades, with of eddy covariance technology, fluxes farmlands been determined many countries. However, studies are very limited for drip irrigation maize arid regions northwestern China, which covers a large area where mixed mode agriculture grazing is...

Dendrobium catenatum Lindl is a valuable medicinal herb and gardening plant due to its ornamental value special medical value. Low temperature major bottleneck restricting D. expansion towards the north, which influences quality yield of catenatum. In this study, we analysed cold response by RNA-Seq. A total 4302 differentially expressed genes were detected under stress, mainly linked protein kinase activity, membrane transport glycan biosynthesis metabolism pathway. We also identified 4005...

In flowering plants, sexual reproductive success depends on the production of viable pollen grains. However, mechanisms by which QUA QUINE STARCH (QQS) regulates development and how transcriptional activators facilitate transcription QQS in this process remain poorly understood. Here, we demonstrate that INDUCER OF CBF EXPRESSION 1 (ICE1), a basic helix-loop-helix (bHLH) factor, acts as key activator positively expression to increase germination viability Arabidopsis thaliana interacting...

Abstract Fructus hippophae (Hippophae rhamnoides spp. mongolica × Hippophae sinensis) , a hybrid variety of sea buckthorn that serves as the female parent and sinensis male parent, is traditional plant with great potentials economic medical values. Herein, we gained chromosome-level genome about 918.59 Mb, scaffolds N50 reaching 83.65 Mb. Then, anchored 440 contigs 97.17% total sequences onto 12 pseudochromosomes. Next, de-novo homology transcriptome assembly strategies were adopted for gene...

A new heteropolysaccharide was extracted and purified from the fruiting bodies of Cantharellus cibarius Fr. The polysaccharide (CC-1) had a molecular weight 61,056 kDa mainly formed glucose xylose at ratio 5:1. Structure identification CC-1 analysed by combined application total hydrolysis, high performance liquid chromatography (HPLC), methylation analysis, gas chromatography-mass spectrometry (GC-MS), infrared (IR) spectra nuclear magnetic resonance (NMR) spectroscopy. experimental results...

Abstract Background In the agricultural areas of Qinghai-Tibet Plateau, temperature varies widely from day to night during growing season, which makes extreme become one limiting factors crop yield. Turnip ( Brassica rapa var. ) is a traditional Tibet grown in but its molecular and metabolic mechanisms freezing tolerance are unclear. Results Here, based on changes transcriptional levels Tibetan turnip under treatment, expression arginine decarboxylase gene BrrADC2.2 exhibited an accumulative...

Increasing the targeting ability of antifungal proteins towards specific components fungal cells has potential to improve their activity and reduce harmful effects nontarget cells. To obtain effective disease resistance genes against cotton Verticillium wilt, we constructed several fusion genes, in which binding domains chitin, sphingolipid or ergosterol cell wall membrane were individually fused peptide BbAFP1 from entomopathogenic fungus Beauveria bassiana. Transient expression cotyledons...

Teosinte branched1/cycloidea/proliferating cell factor (TCP) gene family members are plant-specific transcription factors that regulate plant growth and development by controlling proliferation differentiation. However, there no reported studies on the TCP in Dendrobium catenatum Lindl. Here, a genome-wide analysis of genes was performed D. catenatum, 25 were identified. A phylogenetic classified into two clades: Class I II. Genes same clade share similar conserved motifs. The GFP signals...

Abstract Background Alternanthera philoxeroides (alligator weed) is a highly invasive alien plant that has continuously and successfully expanded from the tropical to temperate regions of China via asexual reproduction. During this process, continuous decrease in temperature been key limiting environmental factor. Results In study, we provide comprehensive analysis cold tolerance alligator weed transcriptomics. The transcriptomic differences between southernmost population northernmost were...

Pseudogenes are important resources for investigation of genome evolution and genomic diversity because they nonfunctional but have regulatory effects that influence plant adaptation diversification. However, few systematic comparative analyses pseudogenes in closely related species been conducted. Here, we present a turnip (Brassica rapa ssp. rapa) sequence characterize among diploid Brassica species/subspecies. The results revealed the number was greatest oleracea (CC genome), followed by...

Natural selection drives local adaptations of species to biotic or abiotic environmental stresses. As a result, adaptive phenotypic divergence can evolve among related living in different habitats. However, the genetic foundation this process remains largely unknown. Two closely alpine grass species, Stipa capillacea and purpurea, are distributed rainfall regions northern Tibet. Here, we analyzed drought tolerance these two found that S. purpurea was more resistance stress than capillacea....

Cananga odorata is known as a natural perfume tree of the Annonaceae family in Magnoliales. However, its phylogenetic position and molecular mechanisms involved biosynthesis floral volatile organic compounds (VOCs) remain unclear. Here, by combining variety sequencing platforms, we present telomere-to-telomere (T2T) genome C. with 735.83 Mb, which represents highest integrity assembly quality magnoliid plants reported to date. Phylogenetic analysis based on multiple datasets approaches...

Trisubstituted alkenes are valuable intermediates in synthetic organic chemistry. The olefination of acylsilanes is expected to be a useful method for preparing trisubstituted with silyl substituent. However, only...