A5025151403- Gut microbiota and health
- RNA Research and Splicing
- Enzyme Catalysis and Immobilization
- Genomics and Chromatin Dynamics
- Nanoparticle-Based Drug Delivery
- Electrochemical sensors and biosensors
- Cardiovascular Function and Risk Factors
- Fungal Biology and Applications
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic function and diabetes
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Parathyroid Disorders and Treatments
- Nutrition and Health in Aging
- Heart Failure Treatment and Management
- Clostridium difficile and Clostridium perfringens research
- Phytochemistry and Bioactivity Studies
- Tryptophan and brain disorders
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cytokine Signaling Pathways and Interactions
- Medicinal plant effects and applications
- Advanced MRI Techniques and Applications
- Caveolin-1 and cellular processes
- Protein Kinase Regulation and GTPase Signaling
- Plant Gene Expression Analysis
Cleveland Clinic
2023-2025
Nanjing Agricultural University
2025
Cleveland Clinic Lerner College of Medicine
2020-2025
Case Western Reserve University
2025
East China University of Science and Technology
2006-2024
Shanghai Institute of Pharmaceutical Industry
2022
Daping Hospital
2021
Army Medical University
2021
State Council of the People's Republic of China
2019-2020
Czech Academy of Sciences, Institute of Biotechnology
2019-2020
Autism spectrum disorder (ASD) is a severe brain development that characterized by deficits in social communication and restricted, repetitive stereotyped behaviors. Accumulating evidence has suggested gut microbiota disorders play important roles gastrointestinal symptoms neurodevelopmental dysfunction ASD patients. Manipulation of the fecal transplantation (FMT) was recently shown to be promising therapy for treatment various diseases. Here, we performed clinical trial evaluate effect FMT...
Diacylglycerol (DAG) is a second messenger that activates protein kinase C and also occupies central role in phospholipid biosynthesis. Conversion of DAG to phosphatidic acid by regulates the amount route it takes. We used degenerate primers amplify polymerase chain reaction products from cDNA derived human endothelial cells. A product with novel sequence was identified clone 2.6-kilobase an cell library. When transfected truncated version this cDNA, COS-7 cells had marked increase activity,...
This work reports a new strategy in surface fabrication of nano-drug delivery systems to achieve site-specific synergetic chemo-photothermal therapy.
Diacylglycerol (DAG) occupies a central position in the synthesis of complex lipids and also has important signaling roles. For example, DAG is an allosteric regulator protein kinase C, cellular levels may influence variety processes including growth differentiation. We previously demonstrated that human endothelial cells derived from umbilical vein express growth-dependent changes their basal diacylglycerol activity (Whatley, R. E., Stroud, E. D., Bunting, M., Zimmerman, G. A., McIntyre, T....
Arachidonoyldiacylglycerol (20:4-DAG) is a second messenger derived from phosphatidylinositol 4,5-bisphosphate and generated by stimulation of glutamate metabotropic receptors linked to G proteins activation phospholipase C. 20:4-DAG signaling terminated its phosphorylation phosphatidic acid, catalyzed diacylglycerol kinase (DGK). We have cloned the murine DGKɛ gene that showed, when expressed in COS-7 cells, selectivity for 20:4-DAG. The significance synaptic function was investigated mice...
Serine-threonine protein kinases in the ribosomal S6 kinase (rsk or p90rsk) family have been implicated as signaling intermediates cellular response to several growth factors. To investigate molecular diversity of human p90rsk isoforms, mixed degenerate oligonucleotide polymerase chain reaction was used isolate partial rsk cDNAs (1.1 kb). Three closely related were obtained (HU-1, HU-2, HU-3). These are encoded by separate genes based on DNA sequence and distinct patterns seen with genomic...
Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), Cdk9/cyclin T1 heterodimer, has been reported to play critical role in elongation. However, the relationship between actin and P-TEFb still not clear. In this study, was found interact with Cdk9, catalytic subunit P-TEFb, complexes. Using immunofluorescence immunoprecipitation assays, Cdk9 bind G-actin through conserved Thr-186 T-loop. Overexpression vitro kinase...
Abstract Lysine acetylation is pivotal in regulating growth, development, and stress responses across numerous plant species. This modification, mediated by lysine acetyltransferases (KATs) deacetylases (KDACs), both dynamic reversible. Despite the economic significance of grape ( Vitis vinifera ) as a fruit crop, comprehensive insights into its KAT KDAC gene families remain limited. In this study, 8 VvKATs 14 VvKDACs were identified within genome. Detailed analyses their physicochemical...
Cardiac allograft vasculopathy (CAV) affects both epicardial and microvascular coronary arteries, however few studies have characterized dysfunction in this population. Several prior shown positron emission tomography/computed tomography (PET/CT) can be used to screen for CAV, the clinical implications of abnormal blood flow absence CAV is unknown. Our study sought assess prognostic its sub-types, endogen/functional classical/structural, using PET/CT cardiac transplant patients without CAV....
Numerous nanocarriers with pH-responsive properties have been designed and fabricated to reduce the adverse side effects of traditional chemotherapeutics, but these are rarely reversible; this may cause "secondary" on normal tissues, because cannot be sealed again prevent leakage incompletely released drugs after re-entering blood circulation. To overcome limitations, we report herein synthesis a reversibly drug delivery system, which can achieve regulated release in "release-stop-release"...
Site-specific and covalent attachment is the most desirable immobilization strategy, but classic methods typically require genetic engineering or complicated material fabrication, resulting in operational complexity difficulty. Herein, a novel site-specific strategy based on accurately selected sites lipase was developed. Specifically, computer-aided structural analysis of functional groups revealed that lysine residues with free amino were far away from catalytic pocket lid, which suitable...
Roast duck is one kind of representative roast food whose flavor mainly produced by the Maillard reaction. However, some potentially toxic compounds are generated in thermal process and a potential health risk. The aim this work was to analyze effects reaction on safety Chinese traditional food: duck.Ducks with different roasting times (0, 10, 20, 30, 40, 50 60 min) were analyzed. 40 min ducks exhibited an acceptable degree sensory attributes, but showed most abundant aroma compounds....
In this study, we report facile, rapid and stable polyamine TEPA-induced tannic acid (TA) co-deposition system-functionalized magnetic nanoparticles, which may provide an improved nanoplatform for enzyme immobilization.